breseq  version 0.38.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 20,896 N→C ?61Q (CAN→CAG rpsT ← 30S ribosomal protein S20
RA 142,348 N→A ?254* (TNG→TAG)  fhuA → ferrichrome porin FhuA
RA 1,386,237 2 bp→GT intergenic (‑60/+16) lacI ← / ← mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional regulator
RA 1,386,240 2 bp→AA intergenic (‑63/+13) lacI ← / ← mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional regulator
RA 2,086,037 A→C intergenic (‑173/+57) sdiA ← / ← yecC transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein TcyN
MC JC 2,806,542 689 bp→71 bp coding (2‑690/690 nt) ung → uracil‑DNA glycosylase
RA 3,917,708 T→A M1K (ATG→AAG) † rpoZ → DNA‑directed RNA polymerase subunit omega
MC JC 3,917,725 Δ22 bp coding (19‑40/276 nt) rpoZ → DNA‑directed RNA polymerase subunit omega
RA 4,032,695 G→T E271* (GAA→TAA)  rbsB → ribose ABC transporter substrate‑binding protein RbsB
RA 4,272,972 +T intergenic (‑226/‑135) coaA ← / → ECDH10B_RS21215 type I pantothenate kinase/tRNA‑Thr
MC JC 4,296,510 671 bp→72 bp coding (2‑672/672 nt) nfi → deoxyribonuclease V

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_010473 1139614 1141026 1413 14 [13] [13] 14 [pgaD]–[pgaB] [pgaD],pgaC,[pgaB]
* * ÷ NC_010473 1155447 1160082 4636 14 [13] [13] 14 [ycdZ]–[ymdA] [ycdZ],csgG,csgF,csgE,csgD,csgB,csgA,csgC,[ymdA]
* * ÷ NC_010473 1380531 1382046 1516 28 [0] [13] 14 [lacA]–[lacZ] [lacA],lacY,[lacZ]
* * ÷ NC_010473 1384901 1385121 221 15 [13] [13] 14 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_010473 2160525 2163778 3254 14 [13] [13] 14 agn43 agn43
* * ÷ NC_010473 3074089 3074262 174 14 [13] [13] 14 araE/kduD arabinose‑proton symporter AraE/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
* * ÷ NC_010473 3170327 3171676–3170619 293–1350 40 [0] [12] 14 [cmtA]–ECDH10B_RS15765 [cmtA],ECDH10B_RS15765

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_010473 218238 =51 (1.330)53 (1.450) 46/380 0.0 53.3% intergenic (+13/‑193) lpcA/yafJ D‑sedoheptulose 7‑phosphate isomerase/class II glutamine amidotransferase
?NC_010473 = 315947 44 (1.200)intergenic (+81/+143) yaiL/fghA DUF2058 domain‑containing protein/S‑formylglutathione hydrolase
* ? NC_010473 = 26202533 (0.860)46 (1.220) 43/392 0.1 59.2% pseudogene (1/294 nt) xynR DNA‑binding transcriptional repressor XynR
?NC_010473 263472 = 31 (0.820)pseudogene (465/468 nt) xynR DNA‑binding transcriptional repressor XynR
* ? NC_010473 276303 =25 (0.650)56 (1.470) 49/396 0.0 69.2% coding (359/957 nt) paoB aldehyde oxidoreductase FAD‑binding subunit PaoB
?NC_010473 3211930 = NA (NA)intergenic (‑1/‑106) nupG/ECDH10B_RS15990 nucleoside permease NupG/IS4‑like element ISVsa5 family transposase
* ? NC_010473 = 27631227 (0.700)52 (1.380) 49/392 0.0 61.7% coding (350/957 nt) paoB aldehyde oxidoreductase FAD‑binding subunit PaoB
?NC_010473 3199469 = 38 (1.010)pseudogene (665/669 nt) nupG nucleoside permease NupG
* ? NC_010473 285519 =67 (1.750)46 (1.200) 41/398 0.1 40.7% intergenic (‑74/+1) ecpA/ecpR common pilus major fimbrillin subunit EcpA/ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR
?NC_010473 319815 = NA (NA)coding (28/444 nt) ECDH10B_RS01610 transferase
* ? NC_010473 = 28552567 (1.750)41 (1.080) 35/394 0.2 38.2% coding (538/543 nt) ecpR ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR
?NC_010473 = 321143 NA (NA)pseudogene (264/264 nt) ECDH10B_RS23575 acetyltransferase
* ? NC_010473 306751 =35 (0.910)37 (0.970) 31/398 0.4 51.4% intergenic (+101/‑394) ECDH10B_RS25865/pdeL protein YahV/DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
?NC_010473 4640589 = NA (NA)intergenic (‑1257/+13) nanC/ECDH10B_RS23035 N‑acetylneuraminic acid outer membrane channel NanC/IS4‑like element ISVsa5 family transposase
* ? NC_010473 = 30675938 (0.990)46 (1.200) 40/398 0.1 54.8% intergenic (+109/‑386) ECDH10B_RS25865/pdeL protein YahV/DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
?NC_010473 3211929 = NA (NA)pseudogene (1/603 nt) nupG nucleoside permease NupG
* ? NC_010473 1118030 =1 (0.030)31 (0.810) 28/396 0.6 59.6% coding (844/1257 nt) yccE YccE family protein
?NC_010473 = 3213256 41 (1.080)intergenic (+12/+1) ECDH10B_RS15990/ECDH10B_RS15995 IS4‑like element ISVsa5 family transposase/DUF4092 domain‑containing protein
* ? NC_010473 = 11180393 (0.080)31 (0.810) 29/396 0.6 91.2% coding (853/1257 nt) yccE YccE family protein
?NC_010473 3211929 = NA (NA)pseudogene (1/603 nt) nupG nucleoside permease NupG
* ? NC_010473 = 13805300 (0.000)14 (0.600)
+78 bp
13/242 1.1 58.2% coding (91/612 nt) lacA galactoside O‑acetyltransferase
?NC_010473 = 4471165 33 (0.860)intergenic (+109/‑29) groL/yjeI chaperonin GroEL/DUF4156 domain‑containing protein
* ? NC_010473 1455332 =43 (1.120)31 (0.870)
+CGTTACTGGTTTCAT
23/368 0.9 45.6% coding (1/978 nt) pspF phage shock protein operon transcriptional activator
?NC_010473 3365613 = 37 (0.970)intergenic (+244/+370) yhaC/rnpB YhaC family protein/RNase P RNA component class A
* ? NC_010473 1823174 =1 (0.030)30 (0.780) 27/398 0.7 60.6% coding (145/816 nt) mepH peptidoglycan DD‑endopeptidase MepH
?NC_010473 3199469 = 38 (0.990)pseudogene (665/669 nt) nupG nucleoside permease NupG
* ? NC_010473 = 18231821 (0.030)32 (0.840) 28/398 0.6 97.0% coding (153/816 nt) mepH peptidoglycan DD‑endopeptidase MepH
?NC_010473 = 3200797 NA (NA)pseudogene (2/2952 nt) ECDH10B_RS15940 SslE/AcfD family lipoprotein zinc metalloprotease
* ? NC_010473 = 31703260 (0.000)40 (1.050) 37/396 0.2 100% pseudogene (294/1257 nt) cmtA PTS mannitol transporter subunit IICB
?NC_010473 3225914 = NA (NA)coding (33/1137 nt) yghO protein YghO
* ? NC_010473 = 3200797NA (NA)33 (0.860) 30/398 0.5 97.1% pseudogene (2/2952 nt) ECDH10B_RS15940 SslE/AcfD family lipoprotein zinc metalloprotease
?NC_010473 = 3632463 1 (0.030)intergenic (‑112/‑116) ompR/greB two‑component system response regulator OmpR/transcription elongation factor GreB
* ? NC_010473 3211929 =NA (NA)30 (0.790) 27/396 0.7 93.8% pseudogene (1/603 nt) nupG nucleoside permease NupG
?NC_010473 3632455 = 2 (0.050)intergenic (‑104/‑124) ompR/greB two‑component system response regulator OmpR/transcription elongation factor GreB
* ? NC_010473 3816235 =NA (NA)36 (0.950) 33/394 0.3 94.8% intergenic (‑35/‑45) hokA/ECDH10B_RS25725 type I toxin‑antitoxin system toxin HokA/IS3‑like element IS150 family transposase
?NC_010473 4028795 = 2 (0.050)intergenic (+4/‑163) kup/rbsD low affinity potassium transporter Kup/D‑ribose pyranase
* ? NC_010473 = 3817675NA (NA)25 (0.650) 25/398 0.9 100% intergenic (+26/+253) ECDH10B_RS25725/glyS IS3‑like element IS150 family transposase/glycine‑‑tRNA ligase subunit beta
?NC_010473 = 4028799 0 (0.000)intergenic (+8/‑159) kup/rbsD low affinity potassium transporter Kup/D‑ribose pyranase