breseq  version 0.38.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_010473276,9600CT68.8% 87.7 / 73.3 80E130K (GAG→AAG) paoAaldehyde dehydrogenase iron‑sulfur subunit PaoA
*NC_0104731,395,3450AG59.3% 50.0 / 133.0 87E467G (GAA→GGA) ECDH10B_RS07015Tn3‑like element ISEc63 family transposase
*NC_010473218,6690TA51.0% 4.4 / 202.9 104L80Q (CTG→CAG) yafJclass II glutamine amidotransferase
*NC_0104734,658,8880AG38.5% 56.2 / 40.0 39intergenic (+143/+277)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,8340TC37.5% 61.2 / 36.0 41intergenic (+89/+331)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,8800TC37.5% 58.0 / 34.3 41intergenic (+135/+285)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,8200TG36.6% 69.4 / 54.2 41intergenic (+75/+345)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,8280GA36.6% 69.1 / 38.2 41intergenic (+83/+337)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,8290GA36.6% 67.9 / 36.8 41intergenic (+84/+336)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,9080TA35.7% 67.8 / 56.0 42intergenic (+163/+257)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104734,658,9210TG34.2% 68.9 / 48.7 41intergenic (+176/+244)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104731,455,3750TC33.9% 87.7 / 60.3 66intergenic (‑43/‑124)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104731,455,3860TC33.3% 111.3 / 38.8 63intergenic (‑54/‑113)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104734,658,9070GA33.3% 67.0 / 49.8 42intergenic (+162/+258)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104731,455,3830TG32.8% 113.1 / 61.3 62intergenic (‑51/‑116)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104731,455,3880TA32.8% 109.9 / 49.8 61intergenic (‑56/‑111)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104734,658,9350TC32.5% 63.6 / 34.3 40intergenic (+190/+230)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase
*NC_0104731,455,3800TC31.2% 111.3 / 58.3 64intergenic (‑48/‑119)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104731,455,3890TA31.0% 104.6 / 46.9 61intergenic (‑57/‑110)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
*NC_0104734,658,9410AT30.8% 64.6 / 43.3 40intergenic (+196/+224)yjjV/yjjWmetal‑dependent hydrolase/YjjW family glycine radical enzyme activase

Marginal new junction evidence (lowest skew 10 of 462 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_010473 296335 =85 (2.220)23 (0.610) 20/390 1.4 21.9% intergenic (+480/‑47) rclR/ykgE reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
?NC_010473 = 296331 81 (2.160)intergenic (+476/‑51) rclR/ykgE reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
* ? NC_010473 = 139629566 (1.720)24 (0.640) 19/392 1.6 27.1% coding (2350/3009 nt) ECDH10B_RS07015 Tn3‑like element ISEc63 family transposase
?NC_010473 1396299 = 64 (1.700)coding (2354/3009 nt) ECDH10B_RS07015 Tn3‑like element ISEc63 family transposase
* ? NC_010473 = 320249NA (NA)19 (0.500) 18/394 1.7 NA coding (29/914 nt) ECDH10B_RS01625 IS3‑like element IS2 family transposase
?NC_010473 320253 = NA (NA)coding (33/914 nt) ECDH10B_RS01625 IS3‑like element IS2 family transposase
* ? NC_010473 = 383811021 (0.550)17 (0.440) 16/398 2.1 44.7% coding (23/849 nt) yiaJ IclR family transcriptional regulator YiaJ
?NC_010473 3838114 = 21 (0.550)coding (19/849 nt) yiaJ IclR family transcriptional regulator YiaJ
* ? NC_010473 1394899 =72 (1.880)15 (0.400) 15/388 2.2 17.8% coding (954/3009 nt) ECDH10B_RS07015 Tn3‑like element ISEc63 family transposase
?NC_010473 = 1394894 68 (1.820)coding (949/3009 nt) ECDH10B_RS07015 Tn3‑like element ISEc63 family transposase
* ? NC_010473 = 320649NA (NA)17 (0.450) 15/396 2.3 NA coding (429/914 nt) ECDH10B_RS01625 IS3‑like element IS2 family transposase
?NC_010473 320653 = NA (NA)coding (433/914 nt) ECDH10B_RS01625 IS3‑like element IS2 family transposase
* ? NC_010473 = 15603NA (NA)15 (0.400) 15/392 2.3 NA coding (159/1113 nt) ECDH10B_RS00080 IS4‑like element IS421 family transposase
?NC_010473 15607 = NA (NA)coding (163/1113 nt) ECDH10B_RS00080 IS4‑like element IS421 family transposase
* ? NC_010473 305 =33 (0.860)14 (0.380) 13/386 2.7 30.4% intergenic (+50/‑32) thrL/thrA thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I
?NC_010473 = 301 32 (0.860)intergenic (+46/‑36) thrL/thrA thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I
* ? NC_010473 = 198544NA (NA)14 (0.370) 13/396 2.8 NA noncoding (670/1542 nt) ECDH10B_RS00915 16S ribosomal RNA
?NC_010473 198549 = NA (NA)noncoding (675/1542 nt) ECDH10B_RS00915 16S ribosomal RNA
* ? NC_010473 4604784 =29 (0.760)12 (0.320) 12/388 2.9 30.3% coding (896/1218 nt) nanX MFS transporter
?NC_010473 = 4604780 27 (0.720)coding (892/1218 nt) nanX MFS transporter