Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_010473	4,658,908	0	T	A	35.7%	67.8 / 56.0	42	intergenic (+163/+257)	yjjV/yjjW	metal‑dependent hydrolase/YjjW family glycine radical enzyme activase
Reads supporting (aligned to +/- strand):  ref base T (18/9);  new base A (5/10);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 5.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGA  >  NC_010473/4658708‑4659098                                                                                                                                                                                                                    |                                                                                                                                                                                                                    ttcTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTGACGC‑‑ATTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTc                                                                                                                                                                                                                    >  1:8761S59/3‑200 (MQ=17)                acaaattctcgcgccacccGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTCGT‑‑GCG‑CA‑GATGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑AACGCT‑GACGCGTC‑‑‑‑‑‑‑TTATCagac                                                                                                                                                                                               >  1:2475S21/18‑197 (MQ=2)                       gttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CAGACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACaa                                                                                                                                                                                         <  1:764S90/200‑1 (MQ=17)                        tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑g                                                                                                                                                                                       >  1:24229S36/1‑200 (MQ=32)                         ttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGGCATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑g                                                                                                                                                                                       >  1:19333S16/1‑200 (MQ=17)                               acccGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTGGCATGCTTCGCAGTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACaaattct                                                                                                                                                                                    >  1:31922S16/3‑195 (MQ=2)                                                      cACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGT‑C‑GAACCGTAGGCCGGATAA‑GGCg                                                                                                                                                   >  1:22316S5/1‑200 (MQ=17)                                                       aCGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTACGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAAAGT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCg                                                                                                                                                   >  1:12584S25/1‑200 (MQ=17)                                                           cg‑cATCCGGCAGTCGT‑‑GCG‑CA‑GATGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGC‑GTGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCAcg                                                                                                                                             <  1:32027S6/200‑1 (MQ=2)                                                                    tccggcagtcgtgcgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTA‑AA‑TT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCa                                                                                                                                        <  1:1959S76/182‑1 (MQ=2)                                                                        ttG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGAATGCCGACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGCGCTAAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATc                                                                                                                                      <  1:32573S10/200‑1 (MQ=17)                                                                          g‑‑‑gCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAg                                                                                                                                >  1:26551S85/1‑200 (MQ=32)                                                                          agtcgtgcgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCg                                                                                                                                    <  1:22890S9/188‑1 (MQ=2)                                                                            tcgtgcgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATTCTCGCGC‑C‑ACCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGGCCTACAA‑ATTCCC‑GCGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGc                                                                                                                                  >  1:280S3/11‑200 (MQ=2)                                                                            tcgtgcgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGc                                                                                                                                  <  1:24231S32/190‑1 (MQ=2)                                                                              gtgcgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAg                                                                                                                                <  1:3180S58/192‑1 (MQ=2)                                                                              gcg‑cACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGt                                                                                                                               >  1:6875S15/1‑200 (MQ=32)                                                                                 cgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATTCTCGCGC‑C‑ACCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGGCCTACAA‑ATTCCC‑GCGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑g                                                                                                                           <  1:30953S3/195‑1 (MQ=2)                                                                                 cgcagaTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GACGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑g                                                                                                                           <  1:28620S14/195‑1 (MQ=2)                                                                                   acaATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑gc                                                                                                                        >  1:25996S7/1‑200 (MQ=32)                                                                                      aTGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATTCTCGCGC‑C‑ACCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGGCCTACAA‑ATTCCC‑GCGC‑C‑ATCCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑gtgcg                                                                                                                       >  1:285S11/1‑196 (MQ=2)                                                                                       tGCCTGATGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAA‑AT‑‑TT‑ACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑gtgctggga                                                                                                                   <  1:27323S24/200‑9 (MQ=2)                                                                                           tGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATg                                                                                                               >  1:17914S23/1‑200 (MQ=32)                                                                                             aTGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATTCTCGCGC‑C‑ACCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGGCCTACAA‑ATTCCC‑GCGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑GTGCGC‑agatgcc                                                                                                              <  1:496S2/200‑7 (MQ=2)                                                                                              tGCGACGCT‑AACGCGTCTTATCAGGCCTACAAATT‑C‑CCGCGCCATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGACCTACAAATT‑‑CTCACGC‑C‑‑ATCCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑gtgctgggatgggc                                                                                                              >  1:10160S18/1‑187 (MQ=0)                                                                                                         aCGCGTCTTATCAGGCCTACAAATT‑C‑GCGC‑C‑ACCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCA‑GATGCCTGA‑TGC‑GACGCT‑AACGCGTC‑‑‑‑‑‑‑TTATCAGGCCTACAA‑ATTCCC‑GCGC‑C‑‑ATCCGTAGGCCGGATAAAGGCGTTCACGCCGCATCCGGCAGTC‑‑GTGCGC‑A‑GATGCCTGATGCGACGCt                                                                                                 <  1:19201S149/200‑1 (MQ=2)                                                                                                          cgcgTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCtt                                                                                                >  1:8183S8/1‑200 (MQ=32)                                                                                                                        ccTACAGGTT‑T‑GTGC‑CGGACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCa                                                                                 <  1:15240S23/200‑1 (MQ=17)                                                                                                                         cTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCCT‑ATCAt                                                                                >  1:11331S4/1‑200 (MQ=17)                                                                                                                           aCAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGc                                                                              <  1:8104S94/200‑1 (MQ=32)                                                                                                                            cAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGcc                                                                             <  1:27075S58/200‑1 (MQ=32)                                                                                                                                           aaccgttgggCCGGATAAGGCATTAGCGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTCTTGTGGTTTATTATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTCCGG‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGTCTAc                                                                          >  1:4332S65/9‑200 (MQ=14)                                                                                                                                                         aTAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGcc                                                 <  1:4727S8/200‑1 (MQ=32)                                                                                                                                                              gCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATa                                            >  1:11548S43/1‑200 (MQ=32)                                                                                                                                                                gTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑AAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAg                                          >  1:21091S46/1‑200 (MQ=17)                                                                                                                                                                    aCGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGt                                      >  1:9452S39/1‑200 (MQ=32)                                                                                                                                                                                        gcgcACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAACGCCCGACGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGt                   <  1:32720S16/200‑1 (MQ=17)                                                                                                                                                                                              aaTGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑A‑GGGCGTTAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGgcg              >  1:26832S39/1‑200 (MQ=17)                                                                                                                                                                                               aTGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGca            >  1:7900S44/1‑200 (MQ=32)                                                                                                                                                                                                tGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTTTATCACGCC‑GCAGAGGTTGGGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGca            <  1:15666S6/200‑1 (MQ=17)                                                                                                                                                                                                 gCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGcaca          <  1:8188S6/200‑1 (MQ=32)                                                                                                                                                                                                            gACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGa  >  1:323S39/1‑200 (MQ=17)                                                                                                                                                                                                                    |                                                                                                                                                                                                                    TGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCG‑CATCCGGCAGTTG‑‑‑GCG‑CACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTT‑T‑GTGC‑CGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTG‑GCGCACAATGCCTGA‑TGC‑GACGCTTGTCGCGTC‑‑‑‑‑‑‑TTATCATGCCTACAA‑GT‑‑CT‑GTGC‑C‑GAACCGTAGGCCGGATAA‑GGCGTTCACGCCGCATCCGGCAGTC‑‑G‑GCGC‑ATAATGCCTGATGCGACGCTTGTCGCGTCTT‑ATCATGCCTACAG‑GTTTGTGCC‑GAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGA  >  NC_010473/4658708‑4659098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 49 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑