Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0104731,455,3750TC33.9% 87.7 / 60.3 66intergenic (‑43/‑124)pspF/pspAphage shock protein operon transcriptional activator/phage shock protein PspA
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base C (15/7);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATC  >  NC_010473/1455179‑1455571
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gaTAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑c                                                                                                                                                                                                               <  1:29426S21/200‑1 (MQ=255)
      gCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGCCTGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGtt                                                                                                                                                                                                          <  1:32563S12/200‑1 (MQ=255)
        taaaaacccgcttcggcgggtttttactttggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGg‑cacg                                                                                                                                                                                                            <  1:8090S8‑M1/50‑3 (MQ=255)
        aGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGttatt                                                                                                                                                                                                       >  1:27188S21/1‑197 (MQ=255)
         taaaaacccgcttcggcgggtttttacttggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCgg‑cacgt                                                                                                                                                                                                           <  1:19608S5‑M1/51‑4 (MQ=255)
          gcggcTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCG‑GATAAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGt                                                                                                                                                                                                <  1:11795S2/200‑1 (MQ=255)
            ggcTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGGAAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCA‑GTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCTGAA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGtt                                                                                                                                                                                               >  1:14581S11/1‑200 (MQ=255)
              ccgcttcggcgggtttttacttttggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑atcccgg‑cacgtggccg                                                                                                                                                                                                      >  1:693S17‑M1/145‑192 (MQ=255)
                  cAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGAtt‑‑‑ctt                                                                                                                                                                                     >  1:29801S3/1‑200 (MQ=255)
                   gcttcggcgggtttttattggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑aa‑‑c‑‑‑‑t‑‑‑‑‑‑c‑atcccgg‑cacgtggccaaagat                                                                                                                                                                                                 <  1:22529S6‑M1/61‑14 (MQ=255)
                      cAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAAt                                                                                                                                                                                 >  1:14237S46/1‑200 (MQ=255)
                       aGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATc                                                                                                                                                                                <  1:13596S151/200‑1 (MQ=255)
                       aGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATc                                                                                                                                                                                <  1:28773S62/200‑1 (MQ=255)
                            ttttacttttggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑CTATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAAt                                                                                                                                                                                 >  1:29495S3‑M1/131‑200 (MQ=255)
                            ttacttttggaggggcagaaagatgaatgactgtccacgacactatacccaaaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATc                                                                                                                                                                                >  1:14009S22‑M1/131‑200 (MQ=255)
                             ttACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCt                                                                                                                                                                        >  1:22184S21/1‑200 (MQ=255)
                              tACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCtt                                                                                                                                                                       >  1:24547S31/1‑200 (MQ=255)
                                ccGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTta                                                                                                                                                                     <  1:30859S34/200‑1 (MQ=255)
                                   tttggaggggcagaaagatgaatgactgtccacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATTT‑‑CTTCAATC‑AGA‑TCt                                                                                                                                                                        <  1:1041S92‑M1/77‑1 (MQ=255)
                                               ccGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATa                                                                                                                                                     <  1:12996S20/200‑1 (MQ=255)
                                                cGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATaa                                                                                                                                                    >  1:9423S25/1‑200 (MQ=255)
                                                    gatgaGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATaaaaaa                                                                                                                                                <  1:29469S16/200‑1 (MQ=255)
                                                           aCCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCAc                                                                                                                                         >  1:18093S4/1‑200 (MQ=255)
                                                                tttGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATC‑‑‑TTTCAACCCAGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCaa                                                                                                                                     <  1:28882S5/200‑1 (MQ=255)
                                                                 cacgacactatacccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACg                                                                                                                                        >  1:12745S25‑M1/94‑200 (MQ=255)
                                                                  tGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAAtt                                                                                                                                  >  1:18965S24/1‑200 (MQ=255)
                                                                      gcggtgTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTAt                                                                                                                              >  1:17291S1/6‑200 (MQ=255)
                                                                       aGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCACCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTAtt                                                                                                                             >  1:14626S99/1‑200 (MQ=255)
                                                                         cGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTaa                                                                                                                           <  1:16405S65/200‑1 (MQ=255)
                                                                           gTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAG‑GTGAAATCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTggtatcgac                                                                                                                         <  1:28338S6/200‑9 (MQ=255)
                                                                             gtgcgatccG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑TTTTTTGG‑TTCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTAtt                                                                                                                             >  1:15427S10/9‑200 (MQ=255)
                                                                              ccaaaagaaagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑CA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATaaaaaattggcacgttaaccttgcgtt                                                                                                                           >  1:10240S21‑M1/81‑187 (MQ=255)
                                                                              cGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTTTTAACaagt                                                                                                                      >  1:29509S13/1‑197 (MQ=255)
                                                                              cGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AAAAGATCAATAAATCAAAACAG‑TAAAAAATTGGCACGCAAATTGTATTAACAGt                                                                                                                       <  1:3597S19/200‑1 (MQ=255)
                                                                                      aagcggcttatcggtcagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTcag                                                                                                                   >  1:29928S18‑M1/73‑200 (MQ=255)
                                                                                        aaaCCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGAc                                                                                                             >  1:14880S21/1‑200 (MQ=255)
                                                                                          aCCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAATAAATTATCTTTGTATTTTCTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTG‑‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACa                                                                                                            <  1:28958S26/200‑1 (MQ=255)
                                                                                                    cagttttacctgatgtacgtaataaaccgtctccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCC‑GG‑CATGTGG‑‑‑‑‑CCGA‑GATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAAc                                                                                                   >  1:7502S15‑M1/59‑200 (MQ=255)
                                                                                                     cagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑T‑‑‑‑C‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGaa                                                                                                    >  1:19473S5‑M1/58‑200 (MQ=255)
                                                                                                     cagttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGaa                                                                                                    >  1:25727S54‑M1/58‑200 (MQ=255)
                                                                                                       gttttacctgatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACg                                                                                                  >  1:6967S3‑M1/56‑200 (MQ=255)
                                                                                                          acctctcatgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAg                                                                                               <  1:22769S1‑M1/148‑1 (MQ=255)
                                                                                                             ga‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCaa                                                                                        >  1:1016S41/1‑200 (MQ=255)
                                                                                                             ga‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGGATGAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCaa                                                                                        <  1:5326S6/200‑1 (MQ=255)
                                                                                                              tgatgtacgtaataaaccgttccggcgggtttatgaaaccaaataacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAAt                                                                                           >  1:14813S24‑M1/49‑200 (MQ=255)
                                                                                                                 atgtacgtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTCG‑‑‑‑‑CCGAGGATTTTTCTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAAt                                                                                           <  1:10777S27‑M1/155‑1 (MQ=255)
                                                                                                                       gtaataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAatcaa‑gataca                                                                                 >  1:27534S19‑M1/40‑196 (MQ=255)
                                                                                                                        taataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CC‑TT‑ATT‑‑‑TTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACTCAGAAATCAA‑GAGGacaac                                                                              >  1:475S14‑M1/39‑200 (MQ=255)
                                                                                                                         aataaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑CAAACT‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAATCAA‑GAgg                                                                                   >  1:1058S61‑M1/38‑200 (MQ=255)
                                                                                                                           taaaccgttccggcgggtttatgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AAAAC‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAATCAA‑GAGGacaa                                                                               >  1:10755S30‑M1/36‑200 (MQ=255)
                                                                                                                             tCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTAt                                                                         <  1:23069S21/200‑1 (MQ=255)
                                                                                                                              aaaccgttccggcgggttataaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAA‑TTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAATCAA‑GAGGACAACATtag                                                                         <  1:22367S8‑M1/168‑2 (MQ=255)
                                                                                                                                ttccggcgggtttatatgcaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAATCAA‑GAGGACAACAttagg                                                                        >  1:1227S18‑M1/31‑198 (MQ=255)
                                                                                                                                         ttATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGGGCATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAACAGGACAATCCTGAACGCAGAAATCAAAGAGGACAACATTATGGGTAttttt                                                               <  1:25272S13/200‑1 (MQ=255)
                                                                                                                                               atgaaaccagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGAAatcaa‑gaggacaacattaggccaataggcggagtg                                                         <  1:10635S8‑M1/185‑18 (MQ=255)
                                                                                                                                                 gaaacgagtaacgTGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCCGG‑CACGTGG‑‑‑‑‑CCGAAGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTGTTAACAGTTCAGCAGGACAAGCCTGAACGCAGaaatcaa‑gaggacaacattaggccaaataggccgtaagg                                                       >  1:20877S5‑M1/14‑181 (MQ=255)
                                                                                                                                                    tt‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCg                                                  >  1:21062S37/1‑200 (MQ=255)
                                                                                                                                                                  gaAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATg                                      >  1:6727S76/1‑200 (MQ=255)
                                                                                                                                                                    aaTTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGcc                                    <  1:32043S12/200‑1 (MQ=255)
                                                                                                                                                                            aCTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCaa                            <  1:11509S43/200‑1 (MQ=255)
                                                                                                                                                                               tGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGc                         <  1:9760S5/200‑1 (MQ=255)
                                                                                                                                                                                 ttAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAA‑TTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGttct                      >  1:16554S76/1‑200 (MQ=255)
                                                                                                                                                                                           ttCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAgaga              >  1:15512S3/1‑200 (MQ=255)
                                                                                                                                                                                             cGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTGCGGCTCTGTCGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTag                 <  1:14619S23/200‑1 (MQ=255)
                                                                                                                                                                                                   a‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCGTAAC‑CAAGAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGag      <  1:15537S47/200‑2 (MQ=255)
                                                                                                                                                                                                           t‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCA‑‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGa    <  1:5662S33/200‑1 (MQ=255)
                                                                                                                                                                                                                  c‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTA‑GAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGAt   <  1:6720S16/200‑1 (MQ=255)
                                                                                                                                                                                                                     tCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATc  <  1:17717S13/200‑1 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                                                 
GATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCG‑AAACCTGTTCCAGCACTTCGAGA‑AAGC‑TGTTCGCCTCACCAAGTAAATTATCTTTGTATT‑‑CTGCCATGATGAAATTCGCCACTTGTTAGTGT‑AATTCGCT‑AA‑‑C‑‑‑‑T‑‑‑‑‑‑C‑ATCCTGG‑CATGTTG‑‑‑‑‑CTGTTGATT‑‑‑CTTCAATC‑AGA‑TCTTTATAAATCAAAA‑AGATAAAAAATTGGCACGCAAATTGTATTAACAGTTCAGCAGGACAATCCTGAACGCAGAAATCAA‑GAGGACAACATTATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATC  >  NC_010473/1455179‑1455571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 50 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑