| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_010473 | 4,032,695 | G→T | E271* (GAA→TAA) | rbsB → | ribose ABC transporter substrate‑binding protein RbsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_010473 | 4,032,695 | 0 | G | T | 97.1% | 129.5 / ‑4.4 | 34 | coding (811/891 nt) | rbsB | ribose ABC transporter substrate‑binding protein RbsB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (15/18); minor base . (1/0); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 | |||||||||||
CGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTT > NC_010473/4032501‑4032885 | cGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGG‑CGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCg < 1:29730S31/200‑1 (MQ=255) ttCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAA‑‑‑GCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATaaa < 1:5494S10/200‑1 (MQ=255) aGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATaaa < 1:32428S33/200‑1 (MQ=255) aGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCAATCAGATT‑GACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATaaa > 1:15990S26/1‑200 (MQ=255) tCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCAGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGaaa > 1:32801S16/1‑200 (MQ=255) aaTGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAA‑‑‑GCGGCTAATTATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGt < 1:13344S13/200‑1 (MQ=255) tgatgaAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAg < 1:26725S37/200‑1 (MQ=255) ggCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAgctaa < 1:8878S27/200‑5 (MQ=255) ggCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTGACAGCGGCTCTCGCT‑CAGCTTCCCAATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTaactgtac > 1:26313S5/1‑194 (MQ=255) cgcgcTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTGCA‑CTTACCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCgg > 1:3228S192/1‑200 (MQ=255) tGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCAATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGAt > 1:17142S21/1‑200 (MQ=255) gCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTaa < 1:5282S48/200‑1 (MQ=255) gTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGATAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCag < 1:7268S1/200‑1 (MQ=255) tCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGtt > 1:28064S13/1‑200 (MQ=255) cggatgtgatggtcgttGACGGTACGCCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCa > 1:7250S3/16‑200 (MQ=255) tcg‑tcgGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGt > 1:3423S6/1‑200 (MQ=255) aTTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCG‑CTGACCTGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTg < 1:7034S8/200‑1 (MQ=255) tttGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTg > 1:28201S3/1‑200 (MQ=255) aTGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAc < 1:10169S69/200‑1 (MQ=255) ggCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGt > 1:4663S6/1‑200 (MQ=255) gCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTC‑TAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCa > 1:2269S3/1‑200 (MQ=255) gAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt < 1:15437S66/200‑1 (MQ=255) ggTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGAt < 1:29590S60/200‑1 (MQ=255) atgatgGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTC‑GGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgt > 1:3903S19/1‑200 (MQ=255) atgatgGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATCGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTT‑AT‑TGAACC‑‑‑‑CGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAat < 1:10020S1/200‑1 (MQ=255) aatgGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg > 1:31459S9/2‑200 (MQ=255) atgGCAAACTAGCAGCGACTATCGCT‑CAGCTACCGAATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAGA‑‑‑CCGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAat > 1:13359S7/1‑200 (MQ=255) cagcGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaaa < 1:1591S62/200‑1 (MQ=255) gcGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTG‑AATATAAAGAAAAGc < 1:24600S21/200‑1 (MQ=255) tATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAggg < 1:19949S243/200‑1 (MQ=255) tCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCa < 1:5322S5/200‑1 (MQ=255) aGCTACCCGATCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGcc < 1:7515S8/200‑1 (MQ=255) aTCAGATTGGCGCGAAAGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAaca > 1:17391S35/1‑200 (MQ=255) aaaGGCGTCTAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAtttt > 1:2970S80/1‑200 (MQ=255) | CGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCG‑TCGGATTTGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCT‑CAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTT > NC_010473/4032501‑4032885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 49 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |