| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_010473 | 4,272,972 | +T | intergenic (‑226/‑135) | coaA ← / → ECDH10B_RS21215 | type I pantothenate kinase/tRNA‑Thr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_010473 | 4,272,972 | 1 | . | T | 86.4% | 71.8 / 0.7 | 22 | intergenic (‑226/‑135) | coaA/ECDH10B_RS21215 | type I pantothenate kinase/tRNA‑Thr |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (8/11); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
AAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGT‑‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACC > NC_010473/4272780‑4273157 | aaaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑Gaaaaa < 1:9968S42/200‑1 (MQ=255) atatTTGCCATGTCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGGATGAATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAAGAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAgtg‑t > 1:17671S7/1‑200 (MQ=255) gcgcGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTc < 1:30243S69/200‑1 (MQ=255) ttCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTg < 1:14883S16/200‑1 (MQ=255) cTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGttt < 1:8226S50/200‑1 (MQ=255) aaTGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGGGAGGAAT‑AATAAGA‑AAAA‑TCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGCGAGCATTGTTGAGCACAGTGATGTT‑GAAAAAGCG‑TGCTA‑‑ATCTGCCCTCCGTTGGGGCTGTttctt < 1:24799S28/200‑1 (MQ=255) aaTGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCT‑‑ATCGTGTCGCAt < 1:26655S35/200‑1 (MQ=255) aTGGGCAGGAAGGGCAACACCAGATGGGG‑AGGAAT‑AAT‑‑‑‑‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTGGTGCGACTTTCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCa < 1:1252S29/200‑1 (MQ=255) gCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTT‑CT‑CATCGTGTCGCATAAAAt > 1:8044S8/1‑200 (MQ=255) aaaaaTCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGtt > 1:11559S6/1‑200 (MQ=255) aaaTCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGc > 1:18082S7/1‑200 (MQ=255) tctGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGT‑‑CTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACt < 1:2780S107/200‑1 (MQ=255) tCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCAt < 1:2520S55/200‑1 (MQ=255) ttCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGTATGAAC‑C‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACt < 1:24687S21/200‑1 (MQ=255) gATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGT‑‑TGAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAg < 1:27321S21/200‑1 (MQ=255) aaaaa‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTTTGAAAAAGTG‑TGCTG‑‑ATCTGCCCTCCGTTCGG‑CTGTTT‑TGCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgta > 1:25383S4/1‑200 (MQ=255) aaa‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgtagg > 1:5607S29/1‑200 (MQ=255) aa‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTT‑GA‑CCATGAACTCAGC‑‑GTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTaga > 1:11569S6/1‑200 (MQ=255) aTGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGT‑‑TGAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAAc < 1:27180S13/200‑1 (MQ=255) tAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACt < 1:6455S41/200‑1 (MQ=255) cATTGTTGAGCACGATGATAT‑‑TAAAAAGTG‑TG‑TAG‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAg > 1:27996S22/1‑200 (MQ=255) gAGCACAATGATGTT‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCAcc > 1:3206S6/1‑200 (MQ=255) | AAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGA‑GGGTAACACCAGATGGGG‑AGGAAT‑AATAAGA‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAA‑GAATTATGGTTTAGCAGGAGC‑‑GCATTGTTGAGCACAATGATGT‑‑GAAAAAGTG‑TGCTA‑‑ATCTGCCCTCCGTTCGG‑CTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTC‑GCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACC > NC_010473/4272780‑4273157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 48 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |