| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_010473 | 1,386,240 | 2 bp→AA | intergenic (‑63/+13) | lacI ← / ← mhpR | DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_010473 | 1,386,240 | 0 | G | A | 97.3% | 143.6 / NA | 37 | intergenic (‑63/+14) | lacI/mhpR | DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (18/18); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| * | NC_010473 | 1,386,241 | 0 | C | A | 100.0% | 148.7 / NA | 37 | intergenic (‑64/+13) | lacI/mhpR | DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/18); total (19/18) | |||||||||||
TTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑TGCGCCATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCAC > NC_010473/1386067‑1386421 || tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCT‑CCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAa‑tgca > 1:27182S95/1‑200 (MQ=255) ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGG‑CGTC‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAa‑tgcat < 1:3428S4/200‑1 (MQ=255) cgcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGc < 1:18213S33/200‑1 (MQ=255) cgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCtt > 1:75S15/1‑200 (MQ=255) ttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAT‑CACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCt > 1:32009S36/1‑200 (MQ=255) gCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑ACT‑‑‑CAATCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCgg < 1:31479S11/200‑1 (MQ=255) cTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGcc > 1:4460S4/1‑200 (MQ=255) caccacGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTAATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGTAGAACGTGA‑‑TCCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTg > 1:23064S12/1‑200 (MQ=255) caccacGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAt > 1:7333S27/1‑200 (MQ=255) cacGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCa > 1:16188S2/1‑200 (MQ=255) acGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCaa > 1:1041S137/1‑200 (MQ=255) cgcgGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑c < 1:26449S23/200‑1 (MQ=255) aaCGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATCGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCcttct < 1:12960S54/200‑1 (MQ=255) aCGGTCTGATATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCGAGCGTC‑GATGGTGT‑CAACGTAAA‑T‑AGTGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCcttct > 1:6368S14/1‑200 (MQ=255) gTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGa > 1:26826S35/1‑200 (MQ=255) gTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCT‑CCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGAt > 1:15500S9/1‑200 (MQ=255) g‑‑ATAATAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTg < 1:19036S27/200‑1 (MQ=255) gaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTA‑‑‑‑AAATAACTCT‑‑‑‑‑GGGCGCT‑ATCATGCCATACCGCCAGCGGCTT‑ATCAACATTCAGATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTAccc > 1:22529S51/1‑200 (MQ=255) cacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTAc < 1:2162S11/200‑1 (MQ=255) gACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGa < 1:3372S25/200‑1 (MQ=255) aCATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGG‑CGTC‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAg < 1:5867S3/200‑1 (MQ=255) aCGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGTATCAA‑CCC‑‑‑CTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGc < 1:6412S1/200‑1 (MQ=255) ggTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCGT‑CCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTg > 1:3180S21/1‑200 (MQ=255) gTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTg > 1:15533S14/1‑200 (MQ=255) tttCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑CTCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGt < 1:25727S9/200‑1 (MQ=255) atagcaTTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCTCATGCTTAGATACCGCGAAAGGTTTGTCCAACATTC‑GATGGTGTTAAACGTAAA‑T‑CATGC‑GCTTCGCCT‑CCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGtt > 1:27645S2/5‑200 (MQ=255) acaTTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTC‑ATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAAt < 1:15963S5/200‑1 (MQ=255) acaTTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGC‑ACT‑CGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCC‑‑‑AGATGCTTTTCCGCTGCCTGTTCAATGGTc > 1:25109S6/1‑200 (MQ=255) caTTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAAt > 1:1338S20/1‑200 (MQ=255) tCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGt < 1:14858S7/200‑1 (MQ=255) tGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTTGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCa < 1:14980S13/200‑1 (MQ=255) tctctTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGAAAAATGCATGCCGCTTCGC‑TTTCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCAtata < 1:81S24/200‑1 (MQ=255) tcctTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑CTCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCCGGTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATac < 1:1058S16/198‑1 (MQ=255) ctctTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATac > 1:1959S39/1‑200 (MQ=255) cATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAACCCCTTCTTCAATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAg > 1:16423S8/1‑200 (MQ=255) cATGCCATACCGCGAAAGGTTT‑GTCAACATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGc < 1:2039S149/200‑1 (MQ=255) tACCGCGAAAGGTTT‑GTCAACATTC‑AATGGCGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCAc < 1:16430S12/200‑1 (MQ=255) || TTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTG‑‑ATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTG‑‑‑‑AATTGACTCTCTTCCGGGCGCT‑ATCATGCCATACCGCGAAAGGTTT‑TGCGCCATTC‑GATGGTGT‑CAACGTAAA‑TGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAA‑CCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCAC > NC_010473/1386067‑1386421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 48 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |