Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_010473	20,896	N→C	?61Q (CAN→CAG)	rpsT ←	30S ribosomal protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_010473	20,896	0	N	C	100.0%	159.3 / NA	35	?61Q (CAN→CAG)	rpsT	30S ribosomal protein S20
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base C (17/18);  total (17/18)

TTGACTATCTGGCAACCGGCAGTGTGTTCTCT‑CACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCNTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCGCTTCTTAGCTGATTTGATATTAGCCAAGGTCCAACTCCCA  >  NC_010473/20707‑21091                                                                                                                                                                                                       |                                                                                                                                                                                                    ttGACTATCTGGCAACCGGCAGTGTGTTCTCT‑CACGCATCACAAAAAACAGCAGGCATAAAAAAACC‑GCTTGCGCCGGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTcc                                                                                                                                                                                            <  1:14923S2/200‑1 (MQ=255)      tATCTGGCAACCGGCAGTGTGTTCTCT‑CACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGACTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATc                                                                                                                                                                                      <  1:9502S40/200‑1 (MQ=255)         cTGGCAACCGGCAGTGTG‑‑‑TCT‑‑ACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATTTGGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGc                                                                                                                                                                                <  1:30806S40/200‑1 (MQ=255)                ccGGCAGTGTGTTCTCTTCACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCT‑‑AGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGT‑‑‑‑‑TGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTaa                                                                                                                                                                      >  1:21187S1/1‑200 (MQ=255)                     aGTGTGTTCTCT‑CACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTa                                                                                                                                                                       <  1:9068S23/200‑1 (MQ=255)                        gtgtTCTCT‑CACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTTCAATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTT‑GTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAAt                                                                                                                                                                    >  1:6834S3/1‑200 (MQ=255)                                      cATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTgcag                                                                                                                                                       >  1:7000S3/1‑200 (MQ=255)                                          acaAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑AATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCagcagc                                                                                                                                                   >  1:2357S38/1‑200 (MQ=255)                                                        gCATAAAAAAACC‑GCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCtt                                                                                                                                     <  1:12736S36/200‑1 (MQ=255)                                                         cATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCtt                                                                                                                                     >  1:30614S65/1‑200 (MQ=255)                                                         cATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCtt                                                                                                                                     <  1:27661S18/200‑1 (MQ=255)                                                             aaaaaaCCCGCTTGCGC‑GGGCTTTTTCACAAAGCT‑CAGCAAATT‑GGC‑AT‑AGACCAGTTTGTT‑GATCT‑CTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGc                                                                                                                              >  1:18077S8/1‑200 (MQ=255)                                                              aaaaaCCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATa                                                                                                                                >  1:24260S17/1‑200 (MQ=255)                                                                 aaCCCGCTT‑‑GC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTcagagcctgc                                                                                                                           <  1:1365S1/200‑10 (MQ=255)                                                                  aCCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTg                                                                                                                            >  1:15230S28/1‑200 (MQ=255)                                                                    ccGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCg                                                                                                                          <  1:3817S2/200‑1 (MQ=255)                                                                           cgc‑gGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCTTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACt                                                                                                                    <  1:265S5/200‑1 (MQ=255)                                                                           cgc‑gGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTC‑ATAGCTGCGTATACttt                                                                                                                  <  1:14137S11/200‑1 (MQ=255)                                                                                    ttttCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTCGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTtgatg                                                                                                           >  1:10908S7/1‑200 (MQ=255)                                                                                                     cAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATaga                                                                                          <  1:12774S29/200‑1 (MQ=255)                                                                                                       aaTT‑GGCGATTAAGCCAGTTTGTT‑GATTT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGc                                                                                        <  1:54S108/200‑1 (MQ=255)                                                                                                               gATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCt                                                                                 >  1:9970S15/1‑200 (MQ=255)                                                                                                                     gCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGCAGTTTTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGc                                                                              <  1:29664S2/200‑1 (MQ=255)                                                                                                                        aGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTtgtg                                                                        >  1:30887S47/1‑200 (MQ=255)                                                                                                                         tttgttt‑gATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGc                                                                       >  1:7619S10/6‑200 (MQ=255)                                                                                                                                 gATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAg                                                                <  1:1070S10/200‑1 (MQ=255)                                                                                                                                         gCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTc                                                         >  1:21426S6/1‑200 (MQ=255)                                                                                                                                           aGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAg                                                       >  1:8589S33/1‑200 (MQ=255)                                                                                                                                              cAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACt                                                    <  1:9204S61/200‑1 (MQ=255)                                                                                                                                                        ttATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGgcgcg                                          >  1:29672S60/1‑200 (MQ=255)                                                                                                                                                                         gTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCGCTTCTTAGCTGATTTGa                         >  1:14525S1/1‑200 (MQ=255)                                                                                                                                                                                      aG‑A‑‑CCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTCAATGGCGCGCTTCTTAGCTGATTTGATATTAGCCAAGGt            <  1:30878S21/200‑1 (MQ=255)                                                                                                                                                                                             cTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCGCTTCTTAGCTGATTTGATATTAGCCAAGGTCCaa        <  1:27832S45/200‑1 (MQ=255)                                                                                                                                                                                                tAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCG‑‑‑CTTAGCTGATTTGATATTAGCCAAGGTCCAACTCCCa  >  1:17785S7/1‑200 (MQ=255)                                                                                                                                                                                                   cagcCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCGCTTCTTAGCTGATTTGATATTAGCCAAGGTCCAACTCCCa  <  1:10990S100/200‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                    TTGACTATCTGGCAACCGGCAGTGTGTTCTCT‑CACGCATCACAAAAG‑CAGCAGGCATAAAAAAACCCGCTTGCGC‑GGGCTTTTTCACAAAGCTTCAGCAAATT‑GGCGATTAAGCCAGTTTGTT‑GATCT‑GTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAG‑A‑‑CCTTTAGCAGCNTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGTCGCCAGCTTCGATAGCTGCGTATACTTTCTTGATGAAAGTACGCATCATAGAGCGACGGCTTGCGTTGTGCTTACGAGCCTTTTCAGACTGAATGGCGCGCTTCTTAGCTGATTTGATATTAGCCAAGGTCCAACTCCCA  >  NC_010473/20707‑21091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 48 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑