Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_010473	3,917,708	T→A	M1K (ATG→AAG) †	rpoZ →	DNA‑directed RNA polymerase subunit omega

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_010473	3,917,708	0	T	A	100.0%	180.4 / NA	43	M1K (ATG→AAG) †	rpoZ	DNA‑directed RNA polymerase subunit omega
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/18);  total (25/18)

ATATGATTATCTGATTGTGAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑TGGCACGCGTAACTGT‑TCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAG  >  NC_010473/3917514‑3917892                                                                                                                                                                                                                    |                                                                                                                                                                                          atatGATTATCTGATTGTGAATGATGACTCCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTT‑AAGTA‑AGGCAcg                                                                                                                                                                                     >  1:9658S8/1‑200 (MQ=255)  tatGATTATCTGATTGTGAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑‑AACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCAcgc                                                                                                                                                                                    <  1:1418S176/200‑1 (MQ=255)       ttATCTGATCGTGAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAAAGCAGCGTCATGACGCTTTACTGAGCAAAAAGCTTCGTC‑GGCAGACTGA‑CCC‑GA‑TT‑CAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAG‑A‑AGGCAcgcg                                                                                                                                                                                   <  1:11782S12/200‑1 (MQ=255)               ttGTGAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TC‑GT‑‑‑‑CAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAG‑A‑AGGCACGCGTAACTGT‑Tgtaaccg                                                                                                                                                                   <  1:20294S10‑M1/200‑8 (MQ=255)                 gtgAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaac                                                                                                                                                                     <  1:21512S47‑M1/200‑6 (MQ=255)                          gaCTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGGCAG‑‑CTTTTT‑AGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgac                                                                                                                                                            <  1:6225S31‑M1/200‑15 (MQ=255)                           aCTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacc                                                                                                                                                           >  1:17741S19‑M1/1‑185 (MQ=255)                           aCTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑‑GCAGAC‑CAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGT‑G‑AG‑‑CTTTTTAAAGA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctgg                                                                                                                                                        <  1:26758S8‑M1/200‑19 (MQ=255)                           aCTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑AAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAG‑A‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacct                                                                                                                                                          <  1:21014S34‑M1/200‑17 (MQ=255)                             ttCGATACCGCGTTGACCGATTTGAAGACCA‑TATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctgg                                                                                                                                                        >  1:11546S28‑M1/1‑182 (MQ=255)                                   aCCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactg                                                                                                                                                   >  1:8311S2‑M1/1‑177 (MQ=255)                                        gTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgc                                                                                                                                              >  1:20886S43‑M1/1‑172 (MQ=255)                                                  ttGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GATTTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcg                                                                                                                                     >  1:3346S21‑M1/1‑163 (MQ=255)                                                   tGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcg                                                                                                                                   <  1:22155S134‑M1/200‑40 (MQ=255)                                                    gAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgc                                                                                                                                  >  1:23860S3‑M1/1‑160 (MQ=255)                                                        aCCATTATTCGCGCCCTGAATGTCTGCGCATGA‑GCCGCC‑‑‑AA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgt                                                                                                                              <  1:7042S7‑M1/200‑45 (MQ=255)                                                         ccATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGTCTgaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtc                                                                                                                              <  1:14272S120‑M1/200‑45 (MQ=255)                                                                    gcC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggta                                                                                                                  <  1:23315S39‑M1/200‑57 (MQ=255)                                                                     cc‑‑GAACGTCTGCGCATGA‑GCCGCCAAAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTTGGGCAGAC‑GAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAAGA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggta                                                                                                                  <  1:4102S80‑M1/200‑57 (MQ=255)                                                                          aaCGTCTGCGCATGACACCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCTCAA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AGGACTTTTTAAGCA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctcgtacgtgtcgcccgcgcgtcgcgctcgtcagatgcaggt                                                                                                                  >  1:31676S29‑M1/1‑144 (MQ=255)                                                                               cTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaag                                                                                                         >  1:6669S2‑M1/1‑135 (MQ=255)                                                                                    cATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatcc                                                                                                    >  1:21798S21‑M1/1‑130 (MQ=255)                                                                                                             cATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgat                                                                              >  1:1801S3‑M1/1‑108 (MQ=255)                                                                                                                 aCGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaa                                                                          >  1:10173S8‑M1/1‑104 (MQ=255)                                                                                                                        aaTCAGCAAA‑‑‑‑TT‑GTT‑GGCA‑AGTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgctcgcgctcgtagatgcaggtaggcggaaaggatccgctggtaccggaagaaaaacgataaaaccactgt                                                                  >  1:13448S26‑M1/1‑96 (MQ=255)                                                                                                                              cAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgc                                                             <  1:31676S8‑M1/200‑110 (MQ=255)                                                                                                                                 a‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGT‑G‑AGA‑CTGTTT‑AGTAGAGGCGCGCGTAACTCT‑Tgtaaccgtttttgacctggtactggtcgccgcgcgtcacgctggtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccaccgtaatcgcgc                                                          >  1:20806S23‑M1/1‑88 (MQ=255)                                                                                                                                        gcaagttgtagcactgact‑ga‑tttcagtatcatgcCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtacccgtttcgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctg                                                         <  1:3348S40‑M1/183‑114 (MQ=255)                                                                                                                                                     gAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaag                                            >  1:24084S54‑M1/1‑74 (MQ=255)                                                                                                                                                     gAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaag                                            >  1:6799S14‑M1/1‑74 (MQ=255)                                                                                                                                                                 tCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgat                                  >  1:19115S4‑M1/1‑64 (MQ=255)                                                                                                                                                                  cAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatc                                 >  1:21428S6‑M1/1‑63 (MQ=255)                                                                                                                                                                    gTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaagctctgatcaa                               <  1:20276S168‑M1/200‑140 (MQ=255)                                                                                                                                                                            cccAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccaga                       <  1:23917S7‑M1/200‑148 (MQ=255)                                                                                                                                                                              cAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatc                     >  1:19942S28‑M1/1‑51 (MQ=255)                                                                                                                                                                               aGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatcc                    >  1:21087S7‑M1/1‑50 (MQ=255)                                                                                                                                                                                gTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaagttgttgatcaacaaccagatcc                   >  1:7449S41‑M1/1‑49 (MQ=255)                                                                                                                                                                                  cATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaaacaaccagattcaa                 <  1:9575S1‑M1/200‑154 (MQ=255)                                                                                                                                                                                      tcttcACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatcctcgacgt             <  1:28695S137‑M1/200‑158 (MQ=255)                                                                                                                                                                                          cACCTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatcctcgacgttcgc         >  1:19032S3‑M1/1‑39 (MQ=255)                                                                                                                                                                                             cTGTGG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatcctcgacgttcgcgaa      >  1:31463S56‑M1/1‑36 (MQ=255)                                                                                                                                                                                                tgG‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaggatccgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccgaatgttcgacgttcgcgaacgc   >  1:27784S10‑M1/1‑33 (MQ=255)                                                                                                                                                                                                 gg‑AG‑‑CTTTTTAAGTA‑AGGCACGCGTAACTGT‑Tgtaaccgttttgacctggtactggtcgccgcgcgtcgcgctcgtcagatgcaggtaggcggaaaaggcaatttcgctggtaccggaagaaaacgataaaaccactgtaatcgcgctgcgcgaaatcgaagaaggtctgatcaacaaccagatcctcgacgttcgcgaa  >  1:11094S11‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                          ATATGATTATCTGATTGTGAATGATGACTTCGATACCGCGTTGACCGATTTGAAGACCATTATTCGCGCC‑‑GAACGTCTGCGCATGA‑GCCGCC‑AAAA‑GCAGCGTCATGACGCTTTAATCAGCAAA‑‑‑‑TT‑GTT‑GGCAGACTGAACCT‑GA‑TTTCAGTATCATGCCCAGTCATTTCTTCACCTGTGG‑AG‑‑CTTTTTAAGTA‑TGGCACGCGTAACTGT‑TCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAG  >  NC_010473/3917514‑3917892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 49 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑