New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_010473	= 3817675	NA (NA)	25 (0.650)	25/398	0.9	100%	intergenic (+26/+253)	ECDH10B_RS25725/glyS	IS3‑like element IS150 family transposase/glycine‑‑tRNA ligase subunit beta
	?	NC_010473	= 4028799	0 (0.000)					intergenic (+8/‑159)	kup/rbsD	low affinity potassium transporter Kup/D‑ribose pyranase

TTAAAGTCGGAGTGTTTTTATCTTGATGAGTTCAGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTG  >  NC_010473/3817485‑3817870                                                                                                                                                                                                        |                                                                                                                                                                                                         ttAAAGTCGGAGTGTTTTTATCTTGATGAGTTCAGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GAt                                                                                                                                                                                                >  1:2551S23/1‑200 (MQ=11)             ttttatcttttttacgaGTTCAGTAATATAAGCGAACTGAAATATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAAAAAAGGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTc                                                                                                                                                                                       <  1:7931S65/185‑1 (MQ=14)                ttttATCTTGATGAGTTCAGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTggg                                                                                                                                                                                >  1:20163S3/1‑200 (MQ=31)                 tttATCTTGATGAGTTCAGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTgggg                                                                                                                                                                               >  1:11174S88/1‑200 (MQ=31)                    aTCTTGATGAGTTCAGTAATATAAGCAAACTGAAGGATGCTGTTACGGAATATATTGAATGCT‑CAGACGAGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTAAAATACACGGAATCAGACCTCTATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTgggg                                                                                                                                                                               >  1:17154S23/1‑200 (MQ=14)                                  aGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGGGCTTTTCTCTGTCTGcc                                                                                                                                                              >  1:21754S16/1‑200 (MQ=17)                                        ataAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCttacgtg                                                                                                                                                       >  1:6811S6/1‑195 (MQ=17)                                        ataAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTATCTCGAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGt                                                                                                                                                      <  1:3572S25/200‑1 (MQ=18)                                                  tGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTga                                                                                                                                            >  1:571S72/1‑200 (MQ=33)                                                     aGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTTTGATTTTATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTgag                                                                                                                                           <  1:1624S2/200‑1 (MQ=18)                                                        aTGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACaaa                                                                                                                                      >  1:18626S50/1‑200 (MQ=34)                                                             gTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTgagaaagtctcaa                                                                                                                                 <  1:27262S5/200‑10 (MQ=18)                                                                  ggAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAg                                                                                                                            <  1:4671S16/200‑1 (MQ=34)                                                                                  tacA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCT‑TGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑a                                                                                                          <  1:18767S149/200‑1 (MQ=25)                                                                                              gaagaaTTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATTATAC‑GATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGA‑TATCTTACACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAgt                                                                                               <  1:24526S43/200‑1 (MQ=25)                                                                                                           gAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTtcttct                                                                                    >  1:7090S39/1‑200 (MQ=38)                                                                                                               ttAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCtt                                                                                >  1:11477S15/1‑200 (MQ=38)                                                                                                                 aaaa‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGa                                                                              <  1:22588S48/200‑1 (MQ=38)                                                                                                                   aa‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTT‑‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCTCACTTTAATAAgttttacgctgcttcttctcc                                                                            >  1:20191S42/1‑183 (MQ=25)                                                                                                                         ggTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGAtata                                                                          <  1:2585S58/200‑1 (MQ=38)                                                                                                                         ggTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGAtata                                                                          <  1:7524S12/200‑1 (MQ=38)                                                                                                                                   cAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTaaaaa                                                                >  1:7532S6/1‑200 (MQ=39)                                                                                                                                                       tgctgtgcaggttccGTGTTTAACTGTCCAACTTTT‑‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAatat                                             >  1:2593S3/15‑200 (MQ=255)                                                                                                                                                        gACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTCGGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCT‑TGTCTGCCTTTTCGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATA‑GTTT‑TTTCT‑CTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAatatat                                           >  1:4221S3/1‑200 (MQ=255)                                                                                                                                                             atatGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAGTACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTTATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAA‑TCTCATAAAATTAATATATgaga                                       <  1:8833S14/200‑1 (MQ=255)                                                                                                                                                              tatGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATgaga                                       >  1:31197S29/1‑200 (MQ=255)                                                                                                                                                                            aaCTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCT‑CTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTcagc                        >  1:31390S9/1‑200 (MQ=255)                                                                                                                                                                                gTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAACTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCaga                     >  1:9685S92/1‑200 (MQ=255)                                                                                                                                                                                    aaCTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTT‑ATTCAGCAGAAGatt                >  1:14503S16/1‑200 (MQ=255)                                                                                                                                                                                           t‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTaaa          <  1:7944S5/200‑1 (MQ=255)                                                                                                                                                                                                    gTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTg  <  1:2950S13/200‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                         TTAAAGTCGGAGTGTTTTTATCTTGATGAGTTCAGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACA‑AC‑AGCAGAAGAATTAGCCTGAAATTAAAA‑‑‑‑GGTCTGACTCCAATTGAA‑TA‑TCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTT‑GGGGTCAGTACAAACTTT‑GATTT‑ATAGTCAGGTGGGG‑CTTTTCTCTGTCTGCCTTT‑CGGTGAATACCTGAGACAAACAGTCTCAAGCACCCGTGGCTATTCT‑AGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTG  >  NC_010473/3817485‑3817870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 47 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.