2023.12.7
We established the SCUT-China-S Wet group.
2023.12.23
We read literature, consulted with PI, and conducted brainstorming sessions within the team to determine the research content of the project.
2024.2.1
Completion of vector construction:
Co-introduction of the ndps1 and ls genes to achieve dual-pathway synthesis of limonene
in yeast.
Construct multiple combinations of ndps1 and ls gene promoters and terminators
(hereinafter referred to as the combination of Promoter1, Terminator1, Promoter2, and
Terminator2 as PTPT).
(Experiment 1.1)
2024.2.9
Fermentation test to select the optimal combination of promoter and terminator:
Construct multiple PTPT-ndps1-ls vectors.
After transformation into yeast, shake-flask fermentation tests were conducted, and from
six biphasic fermentation results, we selected the best promoter and terminator combination.
(Experiment 1.3)
2024.3.1
Using CRISPR/Cas9 genome editing technology, the tHMG1 and IDI1 genes were inserted at the
ROX1 locus in yeast cells, resulting in the ΔROX1::tHMG1, IDI1 strain.
The modified yeast cells were validated in shake-flask experiments, successfully further
increasing limonene production.
(Experiment 2.1)
2024.3.20
Replacing the constitutive ERG20p element with the glucose-induced HXT1p element.
Decoupled cell growth from product synthesis. The modified yeast used mannitol, which is
more abundant in kelp, as a carbon source when glucose was depleted, and energy and
materials flowed more toward limonene synthesis.
(Experiment 3.1)
2024.4.5
Completion of the ERG20F96W mutation, significantly altering the substrate preference of
farnesyl pyrophosphate synthase, further reducing the competition between cell growth and
limonene synthesis, promoting limonene production.
(Experiment 3.2)
2024.4.20
Construction of the yp1062w:
ERG12 gene, accelerating the flux of the MVA pathway.
(Experiment 2.2)
2024.4.23
We recruited members for the art group, publicity group, wiki group, model group, and business group, officially establishing SCUT-China-S.
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2024.4.24
We interviewed Guoyin Lai from Guangzhou Flower Flavours & Fragrances Co., Ltd online, and learned about the application and prospect market of our product limonene.
2024.4.27
We organized the "Running Yeast" orienteering running activity.
Through this activity, we know how much college students know about synthetic biology,
and we also publicize our project.
2024.5.17
We interviewed Dr. Alexander and asked him to shoot a promotional video for our project.
2024.5.19
We participated in the 8th iGEM Southern China Regional Meeting, exchanging project ideas with other iGEMers from the South China region.
More...2024.5.24
The constructed PTPT-ndps1-ls was integrated into the genome from the plasmid to prevent
plasmid loss during cultivation in kelp medium without function.
(Experiment 5.1)
2024.5.31
We communicated with Biocreatech online and obtained 8,500 RMB sponsorship from the company.
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2024.5.31-2024.6.2
We visited the production place of kelp - Nanri Island, Fujian, to understand the growth habits of kelp and the working environment and treatment of kelp workers.
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2024.6.12
We went to Guangzhou Experimental Middle School to popularize synthetic biology, and led the students of the first grade to observe wild yeast cells and learn the related knowledge of cell factories.
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2024.7.11-14
We attended the 11th Conference of China iGEMer Community (CCiC), exchanging project ideas
with iGEMer teams from the Chinese region.
Our project won the most popular poster award.
2024.7.24
We held an exchange meeting with the shanghai-city team.
This exchange provides an opportunity for us to ask questions and seek solutions to
challenges together.
2024.7.31
Completion of long-term cultivation and evolution to select yeast cells that use mannitol as
the sole carbon source, enhancing the yeast's ability to utilize kelp hydrolysate.
(Experiment 4.1)
2024.9.7
We participated in the iSDG (sustainable development) online conference to share the SDG of our project and learn from the SDG of other iGEM teams.
More...2024.9.13
After drying and pulverizing kelp into powder, it was hydrolyzed and enzymatically treated,
followed by filtration to prepare kelp medium for Saccharomyces cerevisiae fermentation.
(Experiment 6.1)
2024.9.20
We achieved a 144-hour fed-batch fermentation in 200 ml scale, with a significant increase
compared to the 10 ml 48-hour shake-flask fermentation.
(Experiment 7.2)
2024.9.22
We performed vacuum rotary evaporation for the limonene and IPM mixture, followed by phase
separation after evaporation, and successfully purified the limonene product.
(Experiment 8.2)
2024.9.22
We held a debate on the theme of environmental protection. In the heat debate, people understand the meaning of environmental protection and what should be done to make it.