1.Safety
In terms of safety, together with teams such as JLU-NBBMS, we have produced a Biosafety and Bioethics
White Paper for iGEMers. In this white paper, each Village has its own section, and each section
includes three parts: Overview of the Village, Team's Project Introduction and Case Study. From our
team's assignment to write climate crisis villages, here are a few things that will help future iGEM
teams:
1. Overview of the Village We presented our team's new idea for the track, namely the use of blue carbon resources. Using synthetic biology, we use kelp as a carbon source for fermentation to provide inspiration and experience for future teams.
2. We list the bioethics and bioregulations that may be involved in this track, summarize and analyze them, and find their correlation with iGEM competition, so as to help future iGEMers to understand the laws and regulations related to biosafety and bioethics in this village, and not forget the principles and norms while making bold innovations. Help them to finish the game more smoothly and achieve achievements.
3. We have listed some biosafety switches/designs used by our track team in the past two years, as well as the biosafety designs involved in our project, reflecting diverse biosafety ideas from different teams, and hope that these ingenious ideas can be inherited and innovated in future races.
In short, in the Biosafety and Bioethics White Paper, we summarize and learn from the experience of our predecessors, further explore and innovate our own track, then compile, sort out and summarize these contents, and then show them to all iGEMers. I hope to give some guidance and inspiration to future teams.
1. Overview of the Village We presented our team's new idea for the track, namely the use of blue carbon resources. Using synthetic biology, we use kelp as a carbon source for fermentation to provide inspiration and experience for future teams.
2. We list the bioethics and bioregulations that may be involved in this track, summarize and analyze them, and find their correlation with iGEM competition, so as to help future iGEMers to understand the laws and regulations related to biosafety and bioethics in this village, and not forget the principles and norms while making bold innovations. Help them to finish the game more smoothly and achieve achievements.
3. We have listed some biosafety switches/designs used by our track team in the past two years, as well as the biosafety designs involved in our project, reflecting diverse biosafety ideas from different teams, and hope that these ingenious ideas can be inherited and innovated in future races.
In short, in the Biosafety and Bioethics White Paper, we summarize and learn from the experience of our predecessors, further explore and innovate our own track, then compile, sort out and summarize these contents, and then show them to all iGEMers. I hope to give some guidance and inspiration to future teams.
The Biosafety and Bioethics Whitepaper cover
Click here to view the full version of the white paper
2.Part
Based on our library, we conducted combinatorial fermentation tests on basic parts. For instance, we
tested the promoter-terminator combinations in our PTPT work. Our tests provide data for future iGEM
teams. Here are some of our parts: RPL3t (yeast terminator).
| Part ID | Name |
|---|---|
| BBa_K5409711 | RPL3t |
3.Kelp processing method
The utilization of effective components in kelp has always been a significant challenge. Our team
combined previous methods with innovation, testing the changes in glucose, mannitol, and sodium alginate
content in kelp hydrolysate under different treatment conditions. We have selected the optimal treatment
plan to fully release and utilize kelp components. Meanwhile, the various organic matter content testing
methods used in this process also provide a reference for subsequent projects related to kelp biomass
utilization.
Click here to view our method
4.Troubleshooting
Kelp is a blue carbon source that can absorb a large amount of carbon dioxide and is considered a
sustainable third-generation biomass resource. However, its bioconversion potential has not yet been
developed. To expand the application of kelp, we have applied synthetic biology techniques. We have
solved the following issues that can help future iGEM teams:
4.1. Problem:
There is no suitable pretreatment process for producing high-nutrient culture media.
Solution: To enable yeast to better utilize organic carbon sources from kelp for growth and fermentation, we have processed kelp hydrolysate through a series of steps, including acid hydrolysis, enzymatic hydrolysis, concentration, and nitrogen supplementation, to find the most suitable kelp culture medium for yeast growth.
4.2. Problem:
The value of products directly converted from kelp is low.
4.1. Problem:
There is no suitable pretreatment process for producing high-nutrient culture media.
Solution: To enable yeast to better utilize organic carbon sources from kelp for growth and fermentation, we have processed kelp hydrolysate through a series of steps, including acid hydrolysis, enzymatic hydrolysis, concentration, and nitrogen supplementation, to find the most suitable kelp culture medium for yeast growth.
4.2. Problem:
The value of products directly converted from kelp is low.
5.Modeling
We have established three models: a kelp growth model, a fermentation process carbon emission model, and
a carbon utilization model. Our models have constructed a carbon footprint and a carbon conversion rate
model path. Future iGEM teams related to environmental protection villages, if their work is designed
for bioconversion, can calculate and evaluate the carbon footprint and carbon conversion rate of their
projects based on our flowcharts and articles, to quantify the carbon economy of their projects.
Click here to view our model
