On this page, we present the different DNA parts that shaped our project. Synthetic biology is built on bricks, genetic elements, all well documented, that can be replicated and used by scientists world-wide. These elements are easy to find for model organisms. This is not the case for the chassis of our biostimulant, P. fluorescens. This is why we are happy to provide the community with a list of the parts that we built for this promising organism. We hope they will be useful for future iGEM projects and synthetic biologists all over the world.

Introduction

The wild-type strain Pseudomonas fluorescens SBW25 does not grow on creatinine

All biobricks were designed on Benchling and most of them were synthesized by IDT, which allowed us to codon-optimize them for Pseudomonas putida, the closest host organism to our P. fluorescens. Other parts were derived from the genome of P. fluorescens SBW25 via PCR amplification. All parts were cloned into the indicated vector using In-fusion Assembly.

Best part

Type	Purpose	Link	Name	Representation	Importance in BioMoon	Length (bp)	Status	RFC
Composite	Operon for creatinine metabolization in P. fluorescens	BBa_K5108009	creA-crnA		***	2058	Worked	10

Basic parts

Type	Purpose	Link	Name	Representation	Importance in BioMoon	Length (bp)	Status	RFC
Basic	RBS from Pseudomonas protegens	BBa_K5108006	aprA CHA0		*	24	Worked	10,1000
Basic	RBS from Pseudomonas protegens	BBa_K5108007	hcnA CHA0		*	25	Worked	10,1000
Basic	RBS from Pseudomonas aeruginosa	BBa_K5108008	hcnA PAO1		*	25	Not used	10,1000
Basic	Creatinase to convert creatine into sarcosine	BBa_K5108003	creA		**	1212	Worked	10
Basic	Creatinine amidohydrolase to convert creatinine intro creatine	BBa_K5108004	crnA		**	809	Worked	10
Basic	Chaperone protein involved in the regulations of mRNA in P. fluorescens	BBa_K5108000	hfq		**	809	Successful cloning	10
Basic	Catalase that decomposes H2O2 into water and oxygen from P. fluorescens	BBa_K5108002	katB		*	1542	Successful cloning	10