Overview

All experiment results will be shown in this page.

Target Enzyme

Laccase (multicopper oxidase family protein)

Gene symbol

AMC88_RS25540

Gene description

multicopper oxidase domain-containing protein

Locus tag

AMC88_RS25540

Gene type

protein coding

Organism

Rhizobium phaseoli (strain: R650, biovar: phaseoli, nat-host: Phaseolus vulgaris)

Lineage

Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Chassis: BL21(DE3)

Result

All experiment results will be shown in this page.

Diagnostic enzymatic digestion

Analyze the restriction endonuclease sites on the inserted sequence and the vector backbone, select enzymes that can cut out the appropriate bands to digest the plasmid DNA, run the digested DNA on agarose gel electrophoresis, using EtBrsubstitutes to determine the size of the DNA fragments.

Restriction Enzyme Digestion Analysis Map

ApaLI(1246,497,2438,3426)

Agarose gel electrophoresis pictures

Lane：24

Sequence alignment overview

Conclusion: the sequencing and diagnostic enzymatic digestion confirmed that the construction of our engineering E.coli succeeded.

Detect the activity and the estradiol degradation rate of the induced laccase from the bacterial extract