After we complete the verification of laccase gene expression by engineering bacteria, we begin to try to embed laccase onto our membrane.
The cultivation of engineering Escherichia coli and the induction of laccase will take place on the membrane. After a sufficient period of culturing, laccase will gradually deposit on the membrane and become entangled by its unique structure. Subsequently, all the bacteria on the membrane will be eliminated through appropriate methods. The membrane will then be rinsed thoroughly with buffer solution. In this way, the ultra-high molecular weight polyethylene (UHMWPE) membrane with embedded laccase is successfully obtained. This membrane can potentially have various applications in different fields due to the presence of laccase with its specific enzymatic properties.
the membrane embedded with enzyme and it is also the prototype of our device.
We thickened and lengthened the border of the frame fixing the membrane to enable us to add other components inside the frame.
We add displacement sensors, signal transmitters, and the simple water-quality detection unit inside the frame.
Eventually, we combine several devices units in a frame. The frame is made of materials that can sink underwater, so it can be placed at the bottom of the water. The displacement sensor can prevent the instrument from being stolen and illegal fishing and mining. When criminals engaged in illegal mining and fishing are operating, the mining and fishing devices on their vessels will move our equipment. At this time, the signal transmitter will send the signal to the nearby coast guard unit to help the coast guard locate the crime scene.
The water quality detection unit can simply detect the water quality at the location where the device is deployed, helping with the restoration of the marine ecological environment.