seawater cultivation tank
Overview
In order to verify the effect of laccase in real complex situations. We set up six groups of seagrass ecosystems to explore the impact of estradiol on the growth of seagrass and whether laccase can help seagrass stay healthy under the influence of phenolic compounds (estradiol).
Experimental seagrass: Zostera japonica.
Seasand: Indonesian seasand.
Seawater: Seawater with a salinity of 1.26 configured by seawater purification.
Experiment group setting：
| 0 mg/ml | 0.005mg/ml | 0.01mg/ml | |
|---|---|---|---|
| Control | |||
| Laccase |
Data collection
We randomly took out 18 seaweeds from the seawater cultivation tank. Before planting the seaweeds, we first measured the approximate length of the seaweeds (with an error of 2 cm). The seaweeds were randomly transplanted into the seawater experimental tanks and we ensured three seaweed will be planted in one tank. After seven days of cultivation, the seaweeds were taken out and their lengths were measured. Eventually, we obtained the lengths of the seaweeds after 7-day cultivation. By subtracting the length value measured before 7 days, we obtained the growth difference of the seaweeds.The average quantity of each group's difference was taken for analysis.
Data
| 0 mg/ml | 0.005mg/ml | 0.01mg/ml | |
|---|---|---|---|
| Control | 20 cm | 10cm | 0cm |
| Laccase | 22cm | 15cm | 5cm |
In this data, the death of seaweeds was calculated as a length of 0.
We analyzed the results in GraphPad Prism 9.5.0 by using 2-way ANOVA.
Conclusion
1. Through the results of this statistical analysis, we can confirm that laccase is still effective in a virtual seawater environment. Moreover, the concentration of estradiol selected as the representative of phenolic compounds is quite high. From the results, we can see that the seagrass in the high-concentration estradiol group without the decomposition of laccase all perished. However, this cannot be the direct evidence to prove that estradiol directly affects the growth of seaweeds because the specific mechanism causing this phenomenon is unknown. This can be used as reference data that laccase is still effective in responding to the real environment.
2. We can analyze the inter-group comparison and figures out that laccase has no significant effect on plant growth./p>