Our project aims to revolutionize antimicrobial treatments in agriculture by integrating microneedle patch technology with synthetic biology. The central objective is to develop a sustainable and targeted delivery system for antimicrobial peptides (AMPs). This system will tackle the overuse of antibiotics, reduce environmental contamination, and combat the emergence of antibiotic-resistant bacteria. By leveraging microneedles for localized drug delivery and engineering microorganisms to produce specific AMPs, we aim to create a highly efficient and environmentally friendly antimicrobial strategy tailored for agricultural pathogens.
Figure 1. Outing group photo.
Anent the modern circumstances of the agriculture industry, problems have occurred as we develop. Our journey started with the issue of growing environmental pollutions created by the overuse of antibiotics in modern agriculture behaviors. Being as a high-school student lead team, we had always been enthusiastic about utilizing biotechnology to create sustainable solutions. Our inspiration came from the pressing request to display the crucial issues of antibiotic pollution and the inefficiencies in current agricultural methods. We hope that we may show an impact towards the community by creating such product.
The significance of this project builds from the negative effects of widespread antibiotic use in agriculture, which includes environmental pollution and the increase of antibiotic-resistant superbugs. In traditional antibiotic applications, we commonly see results in excessive use and insufficient targeting, contributing to soil and water contamination. These effects may cause the insecurity of food supply and plant growth. Thus, microneedle technology provides a reliable solution by enabling localized and controlled drug release to reduce the cost of negative externalities. Combining this concept with synthetic biology allows us to design bacteria that specifically targets treatments on such agricultural issues.
Several concerns of we may encounter in environmental impact might include unintended ecological effects and persistence and degradation. The promotion of genetically modified organisms or synthetic biology products if leaked might disrupt the existing ecosystems, causing the unforeseen consequences. Meanwhile, the existence of antimicrobial peptides in the environment and their possible impact on non-target organisms can lead to long-term ecological changes.
To solve such issues, conducting a comprehensive environmental risk assessment is vital for deployment to acknowledge the possible influences on local ecosystems. Securing that our components used for production, encompassing the silk-based microneedles and antimicrobial peptides are biodegradable and does not persist in the environment.
On the other hand of human and animal health, safety concerns of genetically modified bacteria or synthetic biology-derived antimicrobial peptides requires a thorough evaluation. While considerations of antibiotic resistance of continuous use of antimicrobial peptides might inadvertently contribute to the development of resistance in non-target bacteria.
To unravel the safety concerns of genetically modified bacteria and antimicrobial peptides, producing a safety testing on plant treatment with new technology to ensure the safety for human and animal consumption. To implement a monitored result format to ensure the doses and quantity we use is under control, thus preventing development of resistance in agricultural pathogens.
The cost and accessibility of developing and implementing new technologies might limit their accessibility for smaller scale and resource-poor farmers. Negotiating the regulatory landscape for genetically modified organisms and new biotechnology can be difficult and slow down our process of adopting the benefit of this technologies.
Regarding the difficulties of cost considerations, to lower the cost of production and remain same effectiveness of product is what we value a lot. Helping smaller scales farmers to access new technologies with a more affordable price. Working out clear guidelines and approval for processing the new biotechnologies are vital while we try to establish of project. Ensuring the establishment is based on a clear regulatory pathway.
From the beginning, we had strategically outlined our research agenda, focusing on the application of microneedle patch technology in agriculture industry. Conducting extensive preliminary research, resulting in the creation of two distinct reports tailored to different audiences: one for professionals and another for the general public.
Initially, we developed and refined the survey questionnaires. Building on wet team's research, members divided tasks, with some investigating domestic biotechnology companies and others examining international counterparts. After drafting the survey content, we distributed the questionnaires to these companies, aiming to gather relevant data and insights. Thus, enhancing the professional level and basic foundation of our background information.
Figure 2. The part of designed questionnaires.
We then had the opportunity to interview two biology professors and a Ph.D. candidate from ShanghaiTech University. Through the process of preparing interview questions in advance. During the interview, the professors provided in-depth knowledge on relevant applications and nuances of microneedle patch technology, particularly in agriculture. The professors conducted a comprehensive analysis of the technology, offering a detailed overview and constructive feedback. They emphasized the potential of microneedle patch technology for extensive use in modern agriculture, validating the team's research direction.
Figure 3. The picture of interview meeting.
The interview process enriched the team's understanding of specialized knowledge and deepened our comprehension of the research project. Following the interview, we summarized the meeting content and organized the professional information gathered. This enhanced our survey and provided valuable insights into the compatibility of microneedle patches in agriculture, thus establishing a solid foundation for future research endeavors.
In following days, we have made significant contributions by providing experimental data and collecting expert opinions on microneedle patch technology. Conducting thorough analyses of ethical reviews, interviews, and survey responses, ensuring a robust and comprehensive approach to the final research.