We created a wiki with our team project
We have put together a promotion video for our project; (https://video.igem.org/w/kC34UFUCXjVFMfAeCFzPmU)
We filled in the judging forms;
We made a video presentation.
We have created an attribution page to thank all those who helped develop this project and explain how each one was able to contribute.
We have created a project description page, to describe how and why we chose our iGEM project and a complete overview. (LINK DESCRIPTION)
The dry lab contributions of the project include significant advancements in CBD research. First, their proof of concept and literature review highlighted the importance of formulation in enhancing CBD bioavailability and addressed key challenges like its low solubility. This foundation offers valuable insights for further studies on optimizing CBD delivery systems. In terms of kinetic modeling, the team mapped enzyme profiles for crucial steps in the CBD biosynthesis pathway, making the data publicly available to assist future research. We also developed adaptable Python code for bioreactor optimization, enhancing calculation accuracy and user accessibility. Through their human practices contributions, the team advanced cannabinoid research, developed educational booklets and games to promote synthetic biology, and designed lab adaptations to foster inclusivity, ensuring their work is a resource for future iGEM teams. Click here.
We have successfully achieved significant advancements in engineering through a comprehensive approach that integrated optimization, kinetic modeling, bioreactor modeling and plasmid construction. By meticulously designing expression cassettes, we ensured the structural integrity and catalytic activity of pathway enzymes while enhancing their stability. Our efforts to optimize enzyme activity focused on elevating GPP levels, and we successfully constructed cassettes using homologous recombination sites, strategically targeting genes for silencing to enhance overall production. Through kinetic modeling, we gained an in-depth understanding of the enzymatic reactions involved in CBD biosynthesis, identifying optimal substrate concentrations to minimize enzyme inhibition and enhancing the efficiency of CBDA production. Additionally, we have successfully cloned the cassettes into plasmids. Click here.
Throughout the development of our project, we actively engaged with a diverse range of societal sectors to ensure that our outcomes not only meet rigorous technical and scientific standards but also possess substantial social relevance. We collaborated with healthcare professionals, academic institutions, entrepreneurs, patient associations, and legal representatives to strengthen the project's integration with the society as a whole. Furthermore, our dedication to inclusivity drove us to establish numerous partnerships and initiatives aimed at fostering inclusivity, including the development of multiple projects designed to enhance access and support for underrepresented groups. Click here.
Excellence in Synthetic Biology
To achieve excellence in synthetic biology, we developed a comprehensive two-year project focused on advanced modeling, providing key insights into enzyme structure, function, interactions, metabolic pathway modifications and velocity profiles. This led to the construction of optimized, interchangeable parts for efficient CBD production. Additionally, we focused on bioreactor optimization, contributing significantly to CBD research. Moreover, we launched an inclusivity initiative centered on medicinal CBD, synthetic biology, and education, broadening access and awareness in these fields. Click here.