Basic Parts
| Part Number | Name | Type | Part Description |
|---|---|---|---|
| BBa_K5335000 | MS2 CP with SpyTag | Basic | MS2 CP tandem dimer fusion containing SpyTag tag |
| BBa_K5335001 | SpyCatcher-EGFP | Basic | The fusion protein of SpyCatcher with EGFP |
| BBa_K5335002 | MS2 CP loading sequence | Basic | It can bind to MS2 capsid protein and encase in VLP |
| BBa_K5335005 | Spycatcher-linker-Cry6Aa2-linker-CPPs | Basic | SpyCatcher help Cry6Aa2 load onto VLP;CPPs enhances the nematocidal function of Cry6Aa2 |
| BBa_K5335006 | cry6Aa2 gene sequence | Basic | Cry6Aa2 is a crystalloprotein that kills nematodes |
| BBa_K5335007 | SpyTag-6*His | Basic | SpyTag can bind to fusion proteins containing SpyCatcher |
| BBa_K5335009 | KillerRed (Codon optimization) | Basic | This part derivers from BBa_K1184000. We replace codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are compatible with the codon usage bias of our chassis. |
| BBa_K5335011 | Holin | Basic | A 132-amino-acid-residue cytoplasmic membrane protein to lysis Bacillus subtilis. |
| BBa_K5335012 | PGHs | Basic | A peptidoglycan hydrolase derived from bacteriophages. |
| BBa_K5335014 | B.sub terminator | Basic | The terminator of Bacillus subtilis. |
| BBa_K5335015 | GgaA | Basic | A minor wall teichoic acid (WTA) synthase. |
| BBa_K5335016 | TapA | Basic | A protein that can promote the assembly of TasA into amyloid fibrillary structures. |
| BBa_K5335020 | Nlp20 | Basic | A kind of 20-amino acid-resuides small peptide which could trigger PTI in plants. |
| BBa_K5335021 | VDAL | Basic | A plant immune enhancer. |
| BBa_K5335022 | splip | Basic | A prokaryote secretory protein signal peptide signal peptide from Bucilis subtilis. |
| BBa_K5335024 | amcyan1 | Basic | A Cyan fluorescent protein. Previously recorded by BBa_K4645021. |
| BBa_K5335025 | SpyCatcher-VDAL-CPPs (R9-Tag) | Basic | It can induce plant ETI response. |
| BBa_K5335026 | VDAL-6*His | Basic | It can induce plant PTI response. |
| BBa_K5335027 | AmCyan-CPPs(R9-Tag) | Basic | It can be used both to validate the function of the cell-penetrating peptide R9 and as a fluorescently labeled cell-penetrating tag protein. |
| BBa_K5335029 | Spycatcher-CPPs(R9-Tag) | Basic | It can bind to the target protein containing a SpyTag tag, adding CPPs (R9-Tag). |
| BBa_K5335032 | TbrA | Basic | An aconite isomerase from Bacillus thuringiensis. |
| BBa_K5335031 | TbrB | Basic | A tran-saconite transporter derived from Bacillus thuringiensis. |
Composite Parts
| Part Number | Name | Type | Part Description |
|---|---|---|---|
| BBa_K5335003 | MS2 VLP delivery system | Composite | The whole circuit can play the role of VLP assembly, surface display of functional proteins, and the inner inclusion of functional RNA. |
| BBa_K5335008 | T7promoter+Spycatcher-cry6Aa2-cpps+spycatcher-cpps+spytag-6*His+T7terminator | Composite | A complex protein containing SpyCatcher/SpyTag system, Cry6Aa2 and CPPs was formed. |
| BBa_K5335010 | KillerRed-containing suicide switches regulated by the xylose operon | Composite | The component is mainly used to prevent engineered bacteria from escaping into the natural environment. |
| BBa_K5335013 | Holin and PGHs | Composite | This part includes holin and PGHs, capable of performing the function of lysing bacteria. |
| BBa_K5335017 | The genetic circuit containing GgaA and TapA | Composite | The genetic circuit can express GgaA and TapA in large quantities, thereby increasing the thickness of the bacterial biofilm |
| BBa_K5335019 | Splip and VDAL | Composite | The plant immune enhancers composed of VDAL, Nlp20 and Bacillus splip |
| BBa_K5335023 | A fluorescent reporter module initiated by salicylic acid induction | Composite | After induction with salicylic acid, cyan fluorescent protein was expressed and cyan fluorescence was emitted. |
| BBa_K5335028 | T7promoter+Spycatcher-VDAL-CPPs+Spycatcher-CPPs+Spytag-His+T7terminator | Composite | It can induce plant ETI response. |
| BBa_K5335034 | TbrA-TbrB | Composite | The two enzymes respectively isomerize the CAA in the engineered bacteria to TAA and excrete it. |