We created various new genetic parts, specially designed for functioning in Lactic Acid Bacteria and compatible with Biobricks Assembly. We identified strong Lactococcus promoters such as nisA promoter and PTSIIC-celA promoter, and verified their function with experiments. We also designed a CRISPR/Cas9 plasmid specifically for Lactococcus. Feel free to browse through our documented parts linked below!
| Part Number | Name |
|---|---|
| BBa_K5076200 | nisR |
| BBa_K5076201 | nisK |
| BBa_K5076202 | T2 terminator |
| BBa_K5076203 | viaARBS |
| BBa_K5076204 | MCS (Multiple Cloning Site) |
| BBa_K5076205 | pMG36e |
| BBa_K5076206 | pM-Cas |
| BBa_K5076210 | homology sequence edited with nisA |
| BBa_K5076211 | P32 promoter and RBS |
| BBa_K5076212 | sgRNA |
| BBa_K5076000 | PnisA-AcGFP promoter test expression cassette |
| BBa_K5076001 | CRISPR Plan A: MCS-promoter-sgRNA-terminator-homology sequence edited construct |
| BBa_K5076100 | NisA promoter |
| BBa_K5076101 | NisA promoter (optimized) |
| BBa_K5076103 | NisR promoter |
| BBa_K5076104 | NisR promoter (optimized) |
| BBa_K5076105 | NisF promoter |
| BBa_K5076106 | NisF promoter (optimized) |
| BBa_K5076107 | P3 promoter |
| BBa_K5076108 | P5 promoter |
| BBa_K5076109 | P8 promoter |
| BBa_K5076110 | P11 promoter |
| BBa_K5076111 | P48 promoter |
| BBa_K5076112 | PTS-IIC promoter |
| BBa_K5076113 | vioA RBS |
| BBa_K5076114 | AcGFP |
| BBa_K5076205 | pMG36e plasmid backbone |
| BBa_K5076206 | pM-Cas plasmid backbone |
| BBa_K5076555 | PnisA-nisRK enhancer cassette |
| Part Number |
|---|
| ВВа_K5076207 |
| BBa_K5076208 |
| BBa_K5076209 |
