OVERVIEW

Relying on such an engineering model, we succeeded in obtaining universal gene originals in non-model strains that can improve the salinity and base resistance of model strains.

The experiment proves that our engineering is very successful, and such an experimental idea can also be used for mining other elements, we welcome every teams who want to do this work to communicate and use this method.

I will describe the method in detail and show the success we achieved in this page.

1.Model Result

There were two up-regulated differentially expressed genes, sufB (iron-sulfur cluster scaffolding complex subunit) and mnmA (tRNA-specific 2-thiouracilase), and no down-regulated differentially expressed genes among the six strains; there was one up-regulated differentially expressed protein, purM among the six strains.

The six strains of salinity-tolerant bacteria were screened for differentially expressed genes on both the transcriptome and proteome without differentiating between up- and down-regulation, and found that the six strains had 19 differentially expressed genes, the six strains had 21 differentially expressed proteins, and the six strains had five differentially expressed genes on both the transcriptome and proteome.（details are shown in model section）

In summary, a total of 40 genes with high likelihood were screened, and finally, in combination with the metabolomic data, the six most probable universal antisaline elements expressed at transcriptional, translational, and metabolic levels by saline-tolerant bacteria of different genera in response to different saline environments were listed. The results are as follows.

2.Successful of wet lab

Through the above steps, we have found sufB, it can enhance the ability of E.coli to tolerant the saline and base environment.

Engineering bacteria: e coli.BL21 (DE3)-pET-28a (+)-LS21sufB saline-alkali plate medium pH is 8.5.After induction, the bacterial solution is diluted to 10-2, and the inoculation amount in each cell is 30μL.

In order to verify the saline-alkali tolerance of LS21sufB engineering bacteria, four media with different sodium chloride gradients were cultured and observed. Compared with E coli.BL21 (DE3) wild-type, no-load and uninduced engineering bacteria, it was found that the engineering bacteria significantly improved the saline-alkali tolerance. The highest saline-alkali tolerance values of E coli.BL21 (DE3)-pET-28a (+)-LS21sufB were 10%NaCl and pH=8.5./p>