Problem
In today's laboratories and factories, countless medium are cultivated with various microorganisms. E. Coli, as an important model industrial microorganism, has a wide range of applications in pharmaceutical , chemical, agricultural, and experimental fields. A variety of new strategies and technologies for metabolic engineering modification are used to design, construct and optimize e. Coli chemical cell factories. In the process of culture e. Coli may fail to live normally or even die due to over acidic , over alkaline and over saline environment.
Certificate Of Attendance
Background
China has rich access to a diverse ecosystem, saline-alkali land, rare uranium soil, salt lakes, and unique insect resources, which are a natural treasure trove for the study of saline microbial tolerance mechanisms. Microorganisms use cytoplasmic "salt strategy" and "compatible solute strategy" to tolerate salt stress. They rely on several mechanisms in cytoplasmic pH homeostasis to overcome the stress of alkaline pH. Regardless of the adaptive strategies of microorganisms to tolerate salinity stress, they are essentially the regulation of microbial metabolic processes and life activities by resilience components, which make it possible to study the various resilience components of microorganisms.
Northwestern University Invitation
Method
In order to understand the response mechanisms to saline environments shared among different strains and to improve key components of microbial salinity tolerance. We used three Gram-negative strains Halomonas sp. TD01, Halomonas campaniensis LS21, Enterobacter cloacae RS35 screened from the sodic environment, and Gram-positive bacteria Brevibacterium casei G20 screened from the endophytic environment, Bacillus haynesii P19 and six strains of Gram-positive bacteria Micrococcus luteus R17 screened from radiation environment were the test strains. Changes in metabolite composition and concentration of these six strains during different growth periods in normal and saline environments were detected by LC-MS. The common metabolites of the strains in response to the saline environment were analysed from the taxonomic point of view of Gram-positive bacteria, Gram-negative bacteria (strains screened for the sodium environment), endophytes, and Saltomonas. Finally, common genes were mined and screened by multi-omics tools, recombinant plasmids were constructed, their performance in model cells was evaluated, and common components to improve microbial salinity resistance were identified.
Hope
With the development of synthetic biology, we have more and more opportunities to understand the structures and functions of different gene parts. We expect to use synthetic biology analysis to explore the components of acid, base, and salt resistance and to verify them qualitatively and quantitatively. Ultimately, we hope to obtain universal components that are common to different bacterial genera or common to Gram-positive and Gram-negative bacteria, and that can be used to cope with saline and alkaline environments, so as to improve the resistance of engineered strains and facilitate scientific research and industrial production in extreme environments. We also want to make qualitative and quantitative verifications to construct the visualization model, which can assist us in comprehending the relationship between the genetic elements and saline-alkali resistance.
