Competition Deliverables

We have completed and submitted all required deliverables, except for the presentation video, which will be uploaded on October 9, and the judging session, which will take place at the Jamboree.

• Wiki Wiki
• Presentation Video (not uploaded yet)
• Judging Form
• Judging Session (pending)

Project Attributions

All the contributions made by our team members are explained in detail and the support provided by our instructors, PIs, advisors, and external contributors is acknowledged and explained on the attribution form and wiki page.

See Attributions

Project Description

On our project description page, we explain what the goal of our project RhyzUp is, why it’s a useful application of synthetic biology, and what inspired us.

See Project Description

Contribution

On our contribution wiki page, we list what contributions we made for future iGEM teams.

See Contributions

Engineering Success

On our Engineering page (link), we present our engineering design cycles, describe the process of designing, building, and testing our components, as well as the lessons we learned from each iteration.

See Engineering

Human Practices

Our Human Practices page highlights the work of the Human Practices subteam, which consulted with various stakeholders to understand how our project contributes to the United Nations' 17 Sustainable Development Goals (SDGs). In addition, the interviews and research helped to understand the implementation of a potential product and determine the benefits of our project. Ethical, legal and economic constraints of our project were also considered and consumer perceptions were explored. Furthermore, educational efforts were important in the context of human practices.

See Human Practices

New Basic Part

We successfully modified a diguanylate cyclase (DGC) native to Pseudomonas species IsoF to prevent c-di-GMP from binding to the negative allosteric binding, effectively enhancing the enzyme’s activity. Our mutant is a valuable contribution to our project, as this mutation is a novelty not only in our chosen DGC but also in the P. sp. IsoF strain. Our approach can be applied to other DGCs and understanding the enzyme's inhibitory site has many implications for biofilm-related research.

See New Basic Part

Sustainable Development

By addressing some of the core issues facing agriculture today, our project actively contributes to several of the United Nations Sustainable Development Goals (SDGs). To assess these SDGs, we conducted extensive research and spoke with a variety of stakeholders in fields ranging from agrometeorology to crop protection. Learning about agriculture and sustainability has broadened our understanding of not only our project, but also its real-world implications. We believe that our work in sustainable development is a valuable contribution to RhyzUp and to any future iGEM team competing in the Agriculture Village.

See Sustainable Development

Model

Our model is a valuable contribution to the project because it provides a better understanding of the metabolic impact of the engineered modifications made in the wet lab on the bacteria and suggests further improvements. In addition, the simulation of metabolic flux under soil conditions helps us understand how the bacteria would perform in the field, providing important information for the next steps of the project.

See Model