Our HP work is carried out in multiple ways. We have harvested various information that we wanted to understand from engagement with society. In our HP, there are in total of four informal discussions (conversations without fixed questions), five interviews (conversations with fixed questions). We also communicated with researchers in related fields from different regions through meetings (GCEPC).

However, we believe that the form of conduction is not the focus of our HP work. As a team with the goal of industrialization and a desire to address local issues effectively, we have established connections with various scholars, experts, companies that are stakeholders in the industry through our HP work. This has prompted our deep reflections on the project, on related industries in society, and on the long-term impact of the project on sustainable development.

Therefore, in our team's HP interface, we prefer to categorize it based on the communicatee to present a more comprehensive and more logical mindset on HP and project improvement. We will emphasize the involvement of various stakeholders in our team's HP and the influence they have had on us. As a result, our HP page will be unfolded with four key areas predented: 'Researchers', 'Stakeholders', 'Communities', 'Other Teams' . Through these categories, we aim to comprehensively exhibit our HP work, as well as our achievements and reflections within every inspiring communication with the society.

Aaron Ciechanover(Nobel 2004)

Control of Scientific Prospects：Environment and Medical Health

On April 28, 2024 local time, our entire team attended the "Nobel Prize Hero Debate" event held at Xi'an University of Electronic Science and Technology (Guangzhou Research Institute), and had relevant exchanges with Mr. Ciechanover as a guest about the project. He also provided us with friendly guidance from the scientific direction and attitude.

We introduced him to the entire process of our project, from the utilization of carbon dioxide electrochemical derivatives to the modification of metabolic pathways through synthetic biology methods, and then to the production of high value-added products. He showed a strong interest in the chassis bacteria and protein products that we can utilize electrochemical derivatives of carbon dioxide.

He said that our project is a solution that is in line with the world's environmental problems, and it is also the current trend with a bright future.

Of course, in the end, he also taught us to always maintain a passion for research work, truly transform theory into practice, transform projects from laboratories into practical and usable results, and wish us success.







Biography:
Aaron Ciechanover is an Israeli biochemist born in 1947 in Haifa, Israel. He, along with Avram Hershko and Irwin Rose, was awarded the Nobel Prize in Chemistry in 2004 for their groundbreaking discoveries concerning the ubiquitin-proteasome system, a fundamental process in cells for protein degradation and turnover.

Ciechanover obtained his medical degree from the Hebrew University of Jerusalem and later pursued postdoctoral research at Harvard University and the Massachusetts Institute of Technology (MIT). His research primarily focuses on cell biology and protein degradation, particularly the ubiquitin-proteasome pathway.

The ubiquitin-proteasome pathway is crucial for maintaining cellular homeostasis by selectively degrading damaged, misfolded, or unnecessary proteins. Ciechanover and colleagues' work elucidated the molecular mechanisms underlying this process, including the discovery of the ubiquitin-protein ligase system (E1, E2, and E3 enzymes) and the process of ubiquitin tagging and degradation of target proteins. This discovery has profound implications for understanding cell biology, disease mechanisms, and drug development.

In addition to the Nobel Prize, Ciechanover has received numerous other awards and honors, including the Israel Prize in Life Sciences and the Wolf Prize in Medicine. He is widely respected for his contributions to biomedical research and has significantly advanced our understanding of cellular processes and disease mechanisms.

Huimin Yu(Tsinghua)

Highlight Your Novelties

On May 25, 2024 local time, we had a project exchange with Professor Huimin Yu in the field of bioengineering at Tsinghua University after the GCEPC opening ceremony. We showed her the project promotion video and then asked her to provide us with several suggestions on project implementation.

Professor Yu pointed out that our production methodology based on Halomonas is currently a commonly used model. But our raw material utilization method (combining carbon dioxide electrochemical capture and biological liquid fermentation) and some products (tyrian purple) are very novel.

Meanwhile, what we haven't done well is the design description for metabolic pathway modification. We have indeed created T-switch and QS elements that are exclusively applicable to Halomonas, but we have only focused on the use of them without further describing how we have overcome the difficulties in modifying Halomonas.

In response to the professor's suggestions, we have deeply reflected and improved the main line of our project, adopting a strong causal logic to narrate our storyline.

Overall, Professor Yu is very optimistic about the industrialization of our project, so he has written for us the words "using microorganisms to transform the world".








Biography:
Prof. Huimin Yu

Engaged in long-term research on industrial biocatalysis, synthetic biology, microbial metabolic engineering, and biological nanotechnology. Focusing on the scientific foundation and new methods research and industrial production practice of microbial whole cell catalysts and high-performance cell factories, we are committed to developing efficient and universal industrial microbial chassis cells, constructing customized and modular assembly platforms for cell factories and cell catalysts, and achieving intelligent green biological manufacturing. Significant progress has been made in the development of a highly stress resistant microbial cell catalyst platform for the synthesis of amides, carboxylic acids, and chiral pharmaceutical intermediates, enzyme molecule design and modification, efficient synthesis of lipopeptides in recombinant Bacillus subtilis cell factories, efficient synthesis of hyaluronic acid in recombinant Corynebacterium glutamicum cell factories, and research on biological nanotechnology based on M13 phage biological templates. Published nearly 90 SCI indexed papers, authored 5 Chinese and English monographs, and applied for/granted over 40 invention patents in journals such as Metabolic Engineering, ACS Synthetic Biology, Biomecromolecules, New Biotechnology, Applied Microbiology, and Biotechnology. In 2017, he/she was awarded the Second Prize for Technological Invention by the Ministry of Education (First Completion), in 2016, he/she was awarded the Science and Technology Cooperation Award in Dongying City, in 2013, he/she was awarded the Second Prize for Technological Invention by the China Petroleum and Chemical Industry Federation (First Completion), and in 2003, he/she was awarded the Second Prize for Natural Science by the Ministry of Education (Second Completion). In 2008, he was awarded the title of New Century Excellent Talent by the Ministry of Education. In 2007, he was awarded the title of Beijing Science and Technology New Star (Class A). In 2005, he was awarded the title of Outstanding Scholar in Huaxin by Tsinghua University. In 2003, he was awarded the National Excellent Doctoral Dissertation Award.

1.2.3 Carol S K Lin(CityUHK)

Product acquisition and horizontal comparison
On May 26, 2024 local time, we introduced our project to Prof. Carol S K Lin during a poster display in PolyUHK. After understanding the chassis and process of our entire project, she raised questions about the extraction and purification of our fermented products. At the same time, she also questioned the carbon conversion rate and yield of our project during the production process.

At the level of product extraction, we utilized the characteristic that Halomonas cannot tolerate low osmotic pressure, and directly diluted the fermentation broth to release the products from the bacterial body into the fermentation system. As for purification, we did conduct relatively few tests at that time. In the future, we will contact the corresponding factories to design and test the purification process.

We have conducted detailed research on carbon conversion rate and yield issues before, and due to the characteristics of Halomonas, the relevant data of some PHA products and amino acid derivative products do have significant advantages compared to parallel processes. For our other unmeasured products, we need to conduct further measurements and experiments to obtain reliable data support.

Finally, she gave several suggestions for our promotional format and poster layout: first, we can add QR codes below the poster so that interested people can scan the QR codes for a detailed understanding; At the same time, she suggested that we place some horizontal comparison data in a prominent position in order to highlight the advantages of our project in the comparison.





Biography:
Dr. Carol Lin received her Bachelor’s degree in Chemical and Materials Engineering with 1st class honours from the University of Auckland, New Zealand. She was awarded with PhD in 2008 in the research field of Biochemical Engineering at the School of Chemical Engineering and Analytical Science at the University of Manchester, England. After one year as a postdoctoral researcher in the research group of Professor Wim Soetaert at the Centre of Expertise – Industrial Biotechnology and Biocatalysis at the Ghent University in Belgium, she returned to Hong Kong and joined the Department of Chemical and Biomolecular Engineering at the Hong Kong University of Science and Technology as a Visiting Assistant Professor. In 2011, she began her academic career in School of Energy and Environment at City University of Hong Kong, and she was promoted to Associate Professor in 2017.

Dr. Lin’s research group focuses on technological advancement and development of circular waste-based biorefinery for sustainable production of chemicals, materials and fuels, that contributes to reduction of environmental burden of waste disposal and enhancement of resource efficiency. Focusing on these topics, she has secured more than HK$24 million research funding from various sources including the Innovation and Technology Commission (ITC) in Hong Kong and the Hong Kong Research Grant Council (RGC). She has been involved in over 20 research projects as Principal Investigator, which mainly focus on development of integrated biorefinery strategies includes valorization of agricultural residues, food, beverage, plastic and textile wastes.

Dr. Lin has published more than 160 peer-reviewed journal articles including well-recognised journals such as Nature Reviews Earth & Environment, Chemical Society Review, Energy and Environmental Science, Journal of Hazardous Materials, and Chemical Engineering Journal. She was recognised as the World’s top 2% scientists in the fields of ‘Biotechnology’ and ‘Environmental Science’ by Stanford University in 2020-2022. Dr. Lin has received regular invitations to share her research findings in Keynote and Plenary speeches at major international conferences, including Nature Forum on Plastics and Sustainability 2020 and Nature Conference ‘Waste Management and Valorisation for a Sustainable Future’ 2021. She has been involved as an active member of various national and international commissions and committees such as the Scientific Board of the International Sustainable Chemistry Collaborative Centre (ISC3), Germany. She regularly serves as an external assessor for national and international funding institutions including National Research Foundation (NRF) in Singapore, Dutch Research Council (NWO), European Science Foundation (ESF).

Zhenyu Ding(Chinese Academy of Environmental Planning)

Reflecting Our Project From The Perspective of Policy Formulation in Environment

On May 25, 2024 local time, during the poster display of GCEPC, we introduced our project to Director Ding Zhenyu from the Environmental Planning Institute of the Ministry of Ecology and Environment of China, and asked her to give a fair evaluation of our project from a practical perspective of policy formulation.

Director Ding's evaluation revolves around two questions: Has the carbon footprint of your entire process been accurately measured? Which of your product lines has the lowest carbon emissions and the widest market coverage?

Regarding the first question, we will invite Xiaonan Wang（Tsinghua） to conduct a rational evaluation of the energy consumption of the entire project, from electrochemical capture of carbon dioxide to industrial fermentation.

For the second question, we will also comprehensively evaluate from multiple perspectives such as atomic utilization rate and carbon conversion rate to select production lines that meet the requirements. At the same time, optimize and transform other lines at both the genetic and equipment levels to achieve the overall goal of low-carbon.

Finally, Director Ding has high expectations for our project. She said that future environmental protection measures will revolve around the form of chemistry and biology, so as to truly achieve the dual goals of low-carbon and environmentally friendly. Her final words to us: the project will be implemented as soon as possible.







Biography:
Her Research focuses on the evolution of groundwater recharge and hydrogeochemistry. At present, I am mainly engaged in research related to groundwater pollution prevention and control, including groundwater hydrological cycle, groundwater pollution mechanism and identification, groundwater pollution assessment and simulation, hydrogeochemistry, aeration zone hydrology and climate recording, etc. I have undertaken three research projects (one funded by the National Youth Fund), participated in 15 scientific research projects, and published 16 papers, including 9 SCI papers, 7 first author and corresponding papers. I have participated in multiple domestic and international conferences and reported, and have won the Youth Excellent Paper Award and Excellent Report Award.

ProyesLab(Guangzhou and Zhongshan, China.)

Official Website:http://47.115.207.104

Parallel-Bioreactor(Shenzhen, China)

Official Website: https://www.parallel-bioreactor.com

Shenzhen Sieman(Shenzhen, China)

Official Website: https://www.siemantec.com

Medpha(Guangzhou, China)

Official Website:https://www.medpha.cn

Poweredcarbon(Shenzhen, China)

Official Website:https://poweredcarbon.com

Biocretech(Shenzhen, China)

Innovation and Entrepreneurship Lectures（Education）

The academy offers relevant lectures, inviting outstanding guest speakers in the field of biotechnology and investment to share their experiences in innovation and entrepreneurship.

2024 'Babu Stair Climbing Plan' Innovation Fund

South China University of Technology Undergraduate Innovation and Entrepreneurship Fund

GCEPC(Global Chinese Environmental Protection Conference 2024)

SynBio Challenges 2024

iGEMer Communication in Shenzhen Guangming Science City

Chinese International College Students' Innovation Competition 2024

Entry into the Innovation and Entrepreneurship Incubation Base of South China University of Technology