Notebook
2023
December
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Online theoretical learning
Background research
Experiment research
2024
January
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Online theoretical learning
Background research
Experiment research
Febuary
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29
Online theoretical learning
Background research
Experiment research
March
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Online theoretical learning
Experiment research
April
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Online theoretical learning
May
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Online theoretical learning
June
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Online theoretical learning
July
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Preparing culture medium
Construction of plasmid - PCR
Promotion video producing
August
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Construction of plasmid - PCR
Acquiring carrier - shake culture
Plasmid extraction
Enzyme digestion of pPICZαA & Electrophoresis
Enzyme digestion of Target gene & Electrophoresis
Copy and reconnection of plasmid
Monoclonal colony verification
Shake culture
Plasmid extraction from E. Coli
Enzyme digestion & Electrophoresis
Gel extraction & Transformation
Monoclonal colony verification
Shake culture
Enlargement
Protein extraction & Purification & SDS-PAGE
PTVA screening high copy transformants
Promotion video producing
September
Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Yield test
Enzyme activity test
Promotion video producing
Defence preparation
Wiki building
2023
December
  • Online theoretical learning
  • Background research
  • Experiment research
2024
January
  • Online theoretical learning
  • Background research
  • Experiment research
Febuary
  • Online theoretical learning
  • Background research
  • Experiment research
March
  • Online theoretical learning
  • Experiment research
April
  • Online theoretical learning
May
  • Online theoretical learning
June
  • Online theoretical learning
July
  • Preparing culture medium
  • Construction of plasmid - PCR
  • Promotion video producing
August
  • Construction of plasmid - PCR
  • Acquiring carrier - shake culture
  • Plasmid extraction
  • Enzyme digestion of pPICZαA & Electrophoresis
  • Enzyme digestion of Target gene & Electrophoresis
  • Copy and reconnection of plasmid
  • Monoclonal colony verification
  • Shake culture
  • Plasmid extraction from E. Coli
  • Enzyme digestion & Electrophoresis
  • Gel extraction & Transformation
  • Monoclonal colony verification
  • Shake culture
  • Enlargement
  • Protein extraction & Purification & SDS-PAGE
  • PTVA screening high copy transformants
  • Promotion video producing September
  • Yield test
  • Enzyme activity test
  • Promotion video producing
  • Defence preparation
  • Wiki building