Experiments
1.Disposition of medium
2.The construction of plasmid
3.Protein expression
4. Functional Test
2.1 TcDAE, TtDAE, NtDAE, DsDAE
2.2 pPIcZαA
2.3 pPIcZαA-TcDAE
2.4 Transformation E.Coli
2.5 Monoclonal colony verification
2.6 plasmid extraction
2.7 Transformation Pichia pastoris
2.8 Monoclonal colony verification
3.Protein expression
3.1 Amplification culture
3.2 Protein extraction
3.3 Protein purification
3.4 Protein identification-SDS-PAGE
4. Functional Test
4.1Production of D-psicose
4.2 Screening high-yield strains by PTVA