| Encodings | Name | Functions and Descriptions | Type | Length |
| Part:BBa K5044000 - parts.igem.org | T7 terminator | transcription terminator for bacteriophage T7 RNA polymerase | terminator | 48bp |
| Part:BBa K5044001 - parts.igem.org | 6xHis | 6xHis affinity tag | CDS | 18bp |
| Part:BBa K5044002 - parts.igem.org | Tc-ba-miR-CHS1-1 | misc-feature | 138bp | |
| Part:BBa K5044003 - parts.igem.org | pac | stem loop that binds the bacteriophage MS2 coat protein | misc_RNA | 19bp |
| Part:BBa K5044004 - parts.igem.org | lac operator | The lac repressor binds to the lac operator to inhibit transcription in E.coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) | protein_bind | 25bp |
| Part:BBa K5044005 - parts.igem.org | T7 promoter | promoter for bacteriophage T7 RNA polymerase | promoter | 19bp |
| Part:BBa K5044006 - parts.igem.org | lacI promoter | promoter | 78bp | |
| Part:BBa K5044007 - parts.igem.org | lacI | The lac repressor binds to the lac operator to inhibit transcription in E.coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) | CDS | 1083bp |
| Part:BBa K5044008 - parts.igem.org | ori | high-copy-number ColE1/pMB1/pBR322/pUC origin of replication | rep_origin | 589bp |
| KanR | confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes | CDS | 816bp | |
| cp2 | The main function of the viral coat protein (CP) gene is to serve as a template for the synthesis of coat proteins, and to synthesize coat proteins through transcription and translation, and to form a complete virus with the genetic material of the virus. Expressing the viral coat protein gene in plants can prevent the virus from infecting or causing symptoms | misc_feature | 423bp | |
| TAT | HIV-1 TAT (48-60) is a cell-penetrating peptide derived from the human immunodeficient virus (HIV)-1 Tat protein residue 48-60. It has been used to deliver exogenous macromolecules into cells in a non-disruptive way. | misc_feature | 33bp | |
| Part:BBa K5044011 - parts.igem.org | cp1 | binds to a specific stem-loop structure in the viral RNA (Peabody, 1993) | CDS | 387bp |
| Part:BBa K5044012 - parts.igem.org | RBS | efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989) | RBS | 23bp |
| Part:BBa K5044013 - parts.igem.org | GFP | CDS | 719bp | |
| MCS | multiple cloning site | misc_feature | 46bp | |
| T7 tag | epitope tag from a T7 major capsid protein | CDS | 33bp | |
| thrombin site | thrombin recognition and cleavage site | CDS | 18bp | |
| rop | By regulating the interaction of RNA I and RNA II, the replication of pUC ori is reduced, thereby controlling the copy number of the plasmid. | CDS | 192bp | |
| f1 ori | f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis | rep_origin | 456bp | |
| Part:BBa K5044010 - parts.igem.org | psaB | 3' part psaB | misc_feature | 1201bp |
| rps14 | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. | 5'UTR | 303bp | |
| pac | stem loop that binds the bacteriophage MS2 coat protein | CAAT_signal | 19bp | |
| loxP | loxP is a 34bp DNA sequence located in the P1 bacteriophage, consisting of two 13bp inverted repeat sequences and an 8bp asymmetric spacer. This sequence is a specific recognition and binding site for the Cre recombinase enzyme, used to catalyze the DNA strand exchange process. | misc_feature | 34bp | |
| CrPpsbA | The selective marker gene aadA is driven by CrPpsbA. | misc_feature | 273bp | |
| aadA | The Aada gene, also known as the aminoglycosid-3 '-adenylate transferase gene, is a gene found in prokaryotes. The enzyme encoded by this gene has nucleotide transferase activity and is able to modify aminoglycoside antibiotics so that these antibiotics lose the ability to bind to the target and develop resistance. | misc_feature | 792bp | |
| psbZ | Photosystem I exists in the body as trimer and monomer forms. Its structure has been determined to be the most complex membrane protein. The most notable feature of the PSI protein structure is that the auxiliary factor accounts for more than 30% of the total molecular weight of photosystem I. The auxiliary factor not only plays a decisive role in the function of the protein, but also plays an important role in the assembly and structural integrity of PSI. A monomer unit of photosystem I is composed of 127 cofactors and various different proteins (such as PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaG, and 16 other proteins) covalently bound together, and research has shown that the binding sites of most cofactors and proteins are specific and highly conserved.psbZ is a photosynthetic subunit. | misc_feature | 131bp | |
| M13rev | M13 Reverse Amplification Primer | misc_featur | 19bp | |
| amp | Ampicillin resistance genes refer to genes that encode a protein or enzyme for ampicillin resistance. These genes are usually found in the bacteria's chromosomes or plasmids. By expressing resistance genes, bacteria can produce specific enzymes or proteins that degrade or change the structure of ampicillin, thereby preventing the bactericidal effects of the antibiotic on bacteria. | misc_feature | 861bp | |
| M13for | M13 Forward Amplification Primer | misc_featur | 17bp |