Contents

    Moth Magnet Max™(Manufactured Products)

    Spodoptera litura Pheromone Trap

    Project Overview

    HUBU-China  PLASTID PESTICIDES™  is dedicated to developing an innovative pheromone trap for the control of Spodoptera litura, a significant agricultural pest that causes severe damage to crops. By integrating knowledge from biology, engineering, and environmental science, PLASTID  PESTICIDES™ have designed a robust and effective trap that addresses the limitations of existing solutions. This trap not only attracts and captures male moths but also ensures they are effectively eliminated, thus reducing the next generation's population.

    Technical Field

    Moth Magnet Max™ falls within the field of pest control technology, specifically focusing on the design of a pheromone-based trap for Spodoptera litura.

    Background

    Spodoptera litura is a major agricultural pest, with 5-6 generations per year. It primarily damages plant leaves, young shoots, flowers, and fruits, leading to substantial crop losses. Current methods of control often involve the use of pheromone traps, which attract male moths using sex pheromones. However, these traditional traps have several drawbacks, such as:

    • The collection bags at the bottom of the traps can accumulate water, causing them to fall off or be damaged by the insects.
    • The bags have a short lifespan and, once damaged, the captured insects can escape.

    To address these issues, PLASTID  PESTICIDES™ have developed a new trap design that prevents insect damage to the collection bag and ensures the efficient elimination of the pests.

    Moth Magnet Max™ Novel Design

    Moth Magnet Max™includes the following key components:

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    1. Pheromone Lure: A high-efficiency pheromone lure that attracts male Spodoptera litura moths.
    2. Trap Body (Bait Bucket): A durable bucket that houses the pheromone lure and is filled with a liquid (water or a non-toxic solution) to drown the entering moths.
    3. Multiple Insect Entry Units: These units provide multiple entry points for the moths to enter the trap body. They are designed with a series of inward-pointing, elastic spikes that prevent the moths from escaping once they enter.
    4. Sealing Cap: A cap with a vertical hole for inserting a rod, which holds the pheromone lure in place.
    5. Ventilation Holes: Small holes in the trap body allow the pheromone scent to diffuse, attracting more moths.
    6. Removable Cylindrical Sleeve: A sleeve with additional ventilation holes that can be easily inserted and removed for maintenance and replacement of the pheromone lure.

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    Detailed Design

    • Trap Body (Bait Bucket): The bucket is designed with a wide opening at the top, sealed with a cap. The cap has a vertical hole for a rod that holds the pheromone lure. The bucket also has multiple small holes (first ventilation holes) to allow the pheromone scent to spread.
    • Insect Entry Units: Each unit consists of an outer fixing ring and an inner hood with elastic spikes. The inner hood has a small entry hole at the tip of the spikes, allowing moths to enter but preventing them from escaping.
    • Sealing Cap and Rod: The sealing cap fits tightly over the bucket opening and has a vertical hole for a rod. The rod holds the pheromone lure in place, ensuring it remains effective.
    • Cylindrical Sleeve: A removable sleeve with additional ventilation holes (second ventilation holes) is inserted into the bucket. This sleeve holds the pheromone lure and allows for easy replacement.

    Advantages

    • Enhanced Durability: The trap body is designed to be resistant to insect damage, extending its lifespan.
    • Efficient Capture and Elimination: The liquid in the bucket drowns the moths, ensuring they do not escape and reducing the next generation's population.
    • Easy Maintenance: The removable components, such as the cylindrical sleeve and the pheromone lure, make it easy to clean and maintain the trap.
    • Cost-Effective: The simple and effective design keeps the production costs low, making it accessible for widespread use.

    Implementation

    • Assembly: The trap can be easily assembled by attaching the insect entry units to the bucket, inserting the pheromone lure, and securing the sealing cap.
    • Deployment: Place the trap in the field, ensuring it is positioned to maximize the diffusion of the pheromone scent.
    • Monitoring and Maintenance: Regularly check the trap to ensure the pheromone lure is still effective and replace it as needed. Empty and refill the liquid in the bucket to maintain its effectiveness.

    Future Work

    HUBU-China PLASTID PESTICIDES™ plan to further optimize the design by incorporating sensors and IoT (Internet of Things) technology to monitor the trap's performance and provide real-time data. This will enable us to track the population dynamics of Spodoptera litura and adjust PLASTID PESTICIDES™ control strategies accordingly.

    Conclusion

    Moth Magnet Max™ is a significant step forward in the control of this destructive pest. By addressing the limitations of existing traps, PLASTID PESTICIDES™ aim to provide a more durable, efficient, and cost-effective solution for farmers and agricultural communities. PLASTID PESTICIDES™ believe this innovation will contribute to sustainable and environmentally friendly pest management practices.


    Chloro Vortex(Conceptual Devices)

    Device for Enhancing Chloroplast Transformation Efficiency(Conceptual Devices)

    Abstract

    This invention, named ChloroVortex, is a device designed to enhance the efficiency of chloroplast transformation. It features a chamber (2) and a vacuum pump (3), with the chamber connected to the vacuum pump and communicating with the external atmosphere through an air filtration system (4). The ChloroVortex significantly improves the transformation efficiency of chloroplasts, broadening the scope of application in plant genetic engineering technology while reducing transformation time and material costs.

    Introduction

    In recent years, chloroplast transformation has become an important tool in plant genetic engineering, offering unique advantages such as high gene copy number, stable transgene expression, and the prevention of gene flow to related species. However, the efficiency of chloroplast transformation remains a critical bottleneck, especially in recalcitrant plant species. This document describes a novel device, ChloroVortex, that aims to overcome these limitations by facilitating the uptake of foreign DNA into chloroplasts through controlled vacuum conditions.

    Structure Overview

    The ChloroVortex is comprised of several key components:

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    1. Chamber (2) - A sealed container where plant tissues or cells are placed during the transformation process. The chamber is made of custom stainless steel, designed to withstand high pressure and resist corrosion.
    2. Vacuum Pump (3) - A mechanism that creates a vacuum within the chamber to manipulate the internal pressure. The vacuum pump is a high-performance unit suitable for laboratory use.
    3. Air Filtration System (4) - Ensures a sterile environment by filtering air entering the chamber. The system includes a HEPA filter to maintain sterility.
    4. First Valve (5) - Controls the connection between the chamber and the vacuum pump, allowing precise control over the vacuum pressure.
    5. Second Valve (6) - Regulates the connection between the chamber and the external atmosphere through the air filtration system, ensuring a controlled and sterile environment.
    6. Electronic Control Unit (1) - Manages the operation of the vacuum pump and air filtration system, providing precise control over vacuum duration and rate, as well as valve operation. The electronic control unit is a programmable controller.
    7. Tubing (7, 8) - Connects the chamber to the vacuum pump and air filtration system, respectively. The tubing is designed to be pressure-resistant and durable.

    Operation Protocol

    1. Preparation: Prepare the plant tissues or cells and mix them with the appropriate DNA construct.
    2. Placement: Place the prepared materials into the chamber.
    3. Sealing: Ensure the chamber is properly sealed and connected to the air filtration system.
    4. Evacuation: Use the electronic control unit to activate the vacuum pump, creating a vacuum within the chamber.
    5. Restoration: After a predetermined period, restore the chamber to atmospheric pressure using the air filtration system.
    6. Incubation: Incubate the transformed materials according to standard protocols to allow for gene expression.