Our iGEM team focused on enhancing the activity of enzymes for various industrial applications through the use of innovative biotechnological approaches.. Our work offers future iGEM teams validated protocols and experimental data that can be directly applied or adapted for projects involving enzyme-based biotechnological solutions.
Our team utilized surface display techniques to anchor the laccase gene on the surface of bacteria and measured the enzyme's activity in different cellular locations. The experiment demonstrated that laccase anchored on the cell membrane via surface display technology exhibited significantly higher activity. This method provides a promising approach for future iGEM teams studying laccase activity.
Laccase, as a broad-spectrum oxidase, holds potential for a variety of applications. Future teams can explore its use in bioremediation, such as breaking down environmental pollutants like phenols, dyes, and other toxic compounds. It can also be applied in industrial processes, including the degradation of lignin in paper production, and in biofuel generation by improving biomass breakdown. Additionally, biomedical applications are possible, where laccase could be used for developing biosensors or in drug synthesis involving oxidation reactions. Surface display technology enhances the efficiency of these processes, offering a valuable tool for teams working on laccase-related projects.
Our team added lipase to the product, which plays an important role in the digestion, transport and shearing of esters. We make lipase into enzyme preparation, so as to achieve the effect of decontamination of denim cloth. Through the experiment found that lipase on denim cloth also played a flexible role. So then the iGEM team, whose project is related to this aspect of cloth, can consider using lipase to achieve the effect of cloth softening.
Our team incorporated cellulase into the product, which plays an important role in the fraying process of jeans. We successfully expressed cellulase and designed experiments to verify its ability to hydrolyze cellulose. In addition, the optimal pH and temperature of the enzyme reaction were obtained. When pH=5.3 or temperature 30 ℃, the activity of this enzyme is the highest. These experiments complement the contents of the existing Part BBa_K1175006 and can provide experimental reference for the future iGEM team when designing cellulase parts.
Our iGEM team focused on enhancing enzyme activity for industrial applications, providing future teams with valuable protocols and experimental data. We used surface display technology to improve the activity of laccase, which holds potential for applications in bioremediation, industrial processes, and biomedical fields. We also demonstrated the use of lipase for denim softening, offering insights for textile-related projects. Additionally, we contributed new data to part BBa_K1175006 by optimizing cellulase activity for fabric fraying, providing a reference for future teams working with cellulase. These contributions offer practical guidance for enzyme-based biotechnological solutions.
