Modeling

        3 reactions we performed:
    Reaction 1: Excess PFK1
    Vmax=1625uMol/min (w/ excess pfk1)
    [S] or Lab determined substrate conc. = 0.3mM
    Km determined through literature = 20mM
    v=(1625uMol/min)*(4.6mM)/(20mM)+(4.6mM)
    v=303.861789 uMol/min

    Reaction 2(control): No PFK1
    Reaction 1: Excess PFK1
    Vmax=0uMol/min (w/ excess pfk1)
    [S] or Lab determined substrate conc. = 0mM
    Km determined through literature = 0mM
    v=(0uMol/min)*(0mM)/(20mM)+(0mM)
    v=0 uMol/min

    Reaction 3: Less PFK1
    Vmax=100Mol/min (w/ excess pfk1)
    [S] or Lab determined substrate conc. = 1.25mM
    Km determined through literature = 20mM
    v=(100uMol/min)*(3.5mM)/(75mM)+(3.5mM)
    v=41.1764706 uMol/min

    303.86/41.1765= 7.37945187 times increase with the presence of excess PFK1. 

    Time for biofilm formation overall: 24-48 hours for visible EPS
    Average thickness=20uM
    Average length= 80uM
    Average height=5uM
    Volume=l*w*h
    20*80*5= 8000uM3
    36 hours in mins= 2160 min
    8000/2160
    volume/ time= umol/min
    3.7037037 uMol/min 

    This shows that the rate of reaction for the formation of biofilm is less than the rate of reaction for the catalyzed PFK1 reaction indicating that the degradation done by the enzyme will be greater than the rate of formation allowing for the biofilm to be degraded instead of just formed at a lower rate
  

Author links open overlay panelAthel Cornish-Bowden, & AbstractThe year 2013 marked the centenary of the paper of Leonor Michaelis and Maud Menten (Michaelis and Menten. (2014, December 24). One hundred years of michaelis–menten kinetics. Perspectives in Science. https://www.sciencedirect.com/science/article/pii/S2213020914000627