Cutibactierum Acne is a Gram negative bacteirial strain which isn’t succeptable to many antibacteiral drugs due to itts protective endoroxin layer. On top of this, C.Acne quite oftem grows a biofilm, making it harder to destroy. The antimicrobials that do penentrate evnetually are inefective due to bacterial resisitance. Our project is a novel idea to prevent C.Acnes from prevailing by targeting the glucose pathways. By specifically targeting PFK1, a key enzyme in the glycolytic pathway, we can disrupt the metabolic processes essential for biofilm formation and maintenance. This innovative strategy not only aims to inhibit the growth of C. acnes but also to destabilize existing biofilms, making the bacteria more susceptible to antibiotics and immune clearance. Additionally, targeting metabolic pathways like that of PFK1 may reduce the risk of developing antibiotic resistance, as it operates through a different mechanism than traditional antimicrobials. This dual action—impairing biofilm integrity while enhancing antibiotic efficacy—could lead to more effective treatments for chronic skin conditions, particularly in patients where biofilm-associated infections are a significant barrier to recovery. Overall, this approach holds the potential to improve clinical outcomes and pave the way for novel therapeutic interventions against C. acnes-related diseases.

Through various efforts from our team, mainly the sci comm division, we were able to spread awareness of our project to generate more support for it. In doing so, we have effectively armed the public about the issues that cutibacteirum acne causes. This allows the people that we have reached to be more mindful and to be more supportive of efforts for this topic. We have also reached out to the younger generation by hosting events that allowed us to talk about general biology. This allows these kids to be exposed to some high-level topics at a young age, allowing them to develop an inclination or passion towards biology at a young age. Our public book on Amazon allows students to learn about genetics and genes no matter where they are and it allows them to be well versed in their stem interests.

The wiki provides a platform so the general public can view out research and ideas easily. It allows for our research to be clearly conveyed and our project to be displayed in many angles. We hope to use this wiki to contribute to synthetic biology and research in C.acnes.

Hi [name], This is [name] from the [group]. It's a great pleasure to be able to reach out Is there any time in the evenings when we could meet virtually in order to discuss what we plan to do this season? To sum up our project, our study aims to genetically manipulate C. acnes to degrade biofilm by breaking down carbohydrates and inhibiting the GLMU pathway preventing it from producing EPS. Gut microbiota have been successfully manipulated to consume and degrade carbohydrates at a faster pace. Glyceraldehyde-3-phosphate dehydrogenase or GAPDH is a gene that causes degradation of carbohydrates commonly in various organisms. Using a plasmid that increases gene expression of the GAPDH gene, will result in a decrease in the biofilm production. Please let me know a day and time in the evening that works for you. Once again, we greatly appreciate your offer to help. Thanks, [name]

Our template and wiki serve as a valuable resource for future iGEM teams by providing an efficient and visually engaging platform to share information about their projects, events, and educational initiatives. By using this template, teams can effectively advertise workshops, classes, and outreach programs in a professional and organized manner, ensuring that their message reaches a wider audience. The user-friendly design simplifies the process of creating and updating content, allowing teams to focus on their core activities while still maintaining a strong online presence. This tool not only enhances the visibility of team efforts but also fosters collaboration and learning within the global iGEM community by making it easier to share knowledge and promote educational opportunities.