Replace the coat protein gene part of BaMV with AD6 to generate tyrosine hydroxylase, which will catalyze the synthesis of levodopa.
Replace the Triple Gene Block part of BaMV with P19 to prevent RNA silencing caused by siRNA-RISC interaction. Additionally, replace the coat protein gene part with AD6.
Use the pEAQ vector to express ADH rapidly, generating arogenate dehydrogenase to catalyze the synthesis of tyrosine.