Introduction and Overview
The core of the iGEM competition is Parts. It refers to basic building
blocks in the field of synthetic biology(i.e., the BioBricks), typically
DNA sequences, used for constructing biological systems or achieving
specific functions.
The "Heartecho" project is a LIRA-based AND gate system which could detect
multiple miRNAs, then display results in cell-free system. (See
"Project-Description" page for details of project.) In order to prove our
concept, we designed 19 new basic parts and 27 new composite parts this
year, 46 parts in total.
New Basic Parts
The basic parts are the foundation of the gene circuit. Basic parts are
summarized below:
| Name | Short Description | Type | Length (bp) |
|---|---|---|---|
| BBa_K5038000 | RBS | RBS | 8 |
| BBa_K5038001 | linker | RNA | 21 |
| BBa_K5038002 | LIRA_H01 | RNA | 90 |
| BBa_K5038003 | LIRA_H05 | RNA | 90 |
| BBa_K5038004 | LIRA_input01 | RNA | 58 |
| BBa_K5038005 | LIRA_input05 | RNA | 58 |
| BBa_K5038006 | LIRA_input142 | RNA | 23 |
| BBa_K5038007 | LIRA_input210 | RNA | 22 |
| BBa_K5038008 | LIRA_H142_3/20 | RNA | 87 |
| BBa_K5038009 | LIRA_H142_8/15 | RNA | 87 |
| BBa_K5038010 | LIRA_H142_11/12 | RNA | 87 |
| BBa_K5038011 | LIRA_H210_2/20 | RNA | 87 |
| BBa_K5038012 | LIRA_H210_7/15 | RNA | 87 |
| BBa_K5038013 | LIRA_H210_10/12 | RNA | 87 |
| BBa_K5038014 | LIRA_ANDgate1 | RNA | 142 |
| BBa_K5038015 | LIRA_ANDgate2 | RNA | 140 |
| BBa_K5038016 | LIRA_ANDgate5 | RNA | 140 |
| BBa_K5038017 | lacZα | coding | 180 |
| BBa_K5038018 | lacZ | coding | 3075 |
New Composite Parts
| Name | Short Description | Type | Length (bp) |
|---|---|---|---|
| BBa_K5038019 | PT7-LIRA_H01-linker-EGFP-T7TE | device | 896 |
| BBa_K5038020 | PT7-LIRA_H05-linker-EGFP-T7TE | device | 896 |
| BBa_K5038021 | PT7-lacO-LIRA_H01-linker-EGFP-T7TE | device | 924 |
| BBa_K5038022 | PT7-lacO-LIRA_H05-linker-EGFP-T7TE | device | 924 |
| BBa_K5038023 | PT7-LIRA_input01-T7TE | device | 123 |
| BBa_K5038024 | PT7-LIRA_input05-T7TE | device | 123 |
| BBa_K5038025 | PT7-lacO-LIRA_input01-T7TE | device | 151 |
| BBa_K5038026 | PT7-lacO-LIRA_input05-T7TE | device | 151 |
| BBa_K5038027 | PT7-LIRA_H142_3/20-linker-EGFP-T7TE | device | 896 |
| BBa_K5038028 | PT7-LIRA_H142_8/15-linker-EGFP-T7TE | device | 896 |
| BBa_K5038029 | PT7-LIRA_H142_11/12-linker-EGFP-T7TE | device | 896 |
| BBa_K5038030 | PT7-LIRA_H210_2/20-linker-EGFP-T7TE | device | 896 |
| BBa_K5038031 | PT7-LIRA_H210_7/15-linker-EGFP-T7TE | device | 896 |
| BBa_K5038032 | PT7-LIRA_H210_10/12-linker-EGFP-T7TE | device | 896 |
| BBa_K5038033 | PT7-LIRA_ANDgate1-linker-EGFP-T7TE | device | 951 |
| BBa_K5038034 | PT7-LIRA_ANDgate1-linker-lacZ-T7TE | device | 3306 |
| BBa_K5038035 | PT7-LIRA_ANDgate1-linker-lacZα-T7TE | device | 411 |
| BBa_K5038036 | PT7-LIRA_ANDgate2-linker-EGFP-T7TE | device | 949 |
| BBa_K5038037 | PT7-LIRA_ANDgate2-linker-lacZ-T7TE | device | 3304 |
| BBa_K5038038 | PT7-LIRA_ANDgate2-linker-lacZα-T7TE | device | 409 |
| BBa_K5038039 | PT7-LIRA_ANDgate5-linker-EGFP-T7TE | device | 949 |
| BBa_K5038040 | PT7-LIRA_ANDgate5-linker-lacZ-T7TE | device | 3304 |
| BBa_K5038041 | PT7-LIRA_ANDgate5-linker-lacZα-T7TE | device | 409 |
| BBa_K5038042 | PT7-LIRA_input142-T7TE | device | 91 |
| BBa_K5038043 | PT7-LIRA_input210-T7TE | device | 90 |
| BBa_K5038044 | PT7-LIRA_H01-linker-lacZ-T7TE | device | 3257 |
| BBa_K5038045 | PT7-LIRA_H01-linker-lacZα-T7TE | device | 356 |
Used Parts
In our experiments, we used these parts which didn't uploaded from our
team to construct the gene circuits.
| Name | Short Description | Type | Length (bp) |
|---|---|---|---|
| BBa_K4611014 | T7 promoter | promoter | 17 |
| BBa_B0016 | Terminator (T7 RNAP specific, T_Phi) | terminator | 48 |
| BBa_K4236001 | this is a gene to code EGFP | coding | 720 |
| BBa_K3286004 | lac operator | regulatory | 25 |