Design | Tsinghua-M

Part Collection

Part整理

We have submitted a total of 27 basic parts and 10 composite parts, all of which are listed in the following table. The most important ones are marked in brown, indicating that these are parts we have meticulously designed and for which we have conducted detailed characterization. The relatively important ones are marked in blue, these are parts we have designed but for which we have not conducted related characterization experiments. The least importtant ones are marked in black.

本次我们一共提交了27个basic parts和10个composite parts，这些parts全部在以下表格中列出。其中最重要的用棕色标出，这意味着这是我们精心设计的部件，并且对其做了详细的表征。较为重要的用蓝色标出，这些是我们设计的部件，但是没有做相关的表征实验。最不重要的用黑色标出。

Basic parts

This section displays all the basic parts we have submitted. Apart from the two variants of ADAR, all these parts have been used to assemble our sensor RNA and the corresponding target RNA.

这部分展出了我们提交的所有基础部件，除了ADAR的两种变体以外，这些部件全都被用来组装成我们的sensor RNA以及对应的target RNA。

ID	Name	Type	Length(bp)	Introduction
BBa_K5242000	ADAR1_p150	Coding	3717	ADAR1_p150 is one of the crucial enzyme that catalyzes A-to-I editing.
BBa_K5242001	ADAR2_MCP	Coding	1662	ADAR2 is one of the crucial enzyme that catalyzes A-to-I editing. This part contains its deaminase domain and MCP domain.
Bba_K5242009	xylRNLS	Coding	1195	This protein represses pxyl without xylose.
Bba_K5242013	Cre	Coding	546	Cre recombinase is an enzyme that catalyzes recombination events at loxP sites.
Bba_K5242018	GSDMD'N	Coding	825	GSDMD is a suiside gene and this part is its N-domain.
Bba_K5242017	BAX_myc	Coding	618	BAX_myc is a suiside gene.
Bba_K5242016	BAX-20	Coding	516	BAX-20 is a suiside gene.
Bba_K5242019	E2A	Coding	60	E2A can induce ribosomal skipping during translation of a protein in yeast cells.
Bba_K5242020	LV2A	Coding	54	LV2A can induce ribosomal skipping during translation of a protein in yeast cells.
Bba_K5242022	eGFP	Repoter	720	This part is a widely used repoter, with terminal codon UAG instead of UAA.
Bba_K5242003	mScarlet	Repoter	696	This part is a widely used repoter.
Bba_K5242004	eBFP2	Repoter	702	This part is a widely used repoter.
Bba_K5242005	Chk1	RNA	1431	This part is checkpoint kinase 1, and it is used as our target RNA
Bba_K5242006	Chk1s	RNA	1207	This part is a variant of checkpoint kinase 1, and it is used as our target RNA
Bba_K5242008	ogRNA-Chk1	RNA	252	This part is the core to detect Chk1, as it can be edited when Chk1 appears.
Bba_K5242002	ogRNA-Chk1s	RNA	252	This part is the core to detect Chk1s, as it can be edited when Chk1s appears.
Bba_K5242015	ogRNA_GLC3	RNA	252	This part is the core to detect GLC3, as it can be edited when GLC3 appears.
Bba_K5242014	ogRNA_HSP26	RNA	252	This part is the core to detect HSP26, as it can be edited when HSP26 appears..
Bba_K5242021	gRNA	RNA	179	This part is used as target RNA for the pre-experiment.
Bba_K5242030	VMA6 promoter	Regulatory	550	This part is a promoter.
Bba_K5242012	pxyl	Regulatory	138	This part is a promoter.
Bba_K5242031	GAL1,10 promoter	Regulatory	665	This part is a promoter.
Bba_K5242028	TEF1 promoter	Regulatory	407	This part is a promoter.
Bba_K5242032	ECM10 terminator	Terminator	213	This part is a terminator
Bba_K5242010	Tsynth21	Terminator	69	Tsynth21 is a terminator
Bba_K5242011	RiboJ00.S1	Others	157	RiboJ00.S1 is a genetic insulator.
Bba_K5242026	scar1	Scar	8	This part is a scar formed during assembly.

Basic parts from previous teams

来自先前队伍的Basic parts

In the assembly process of the Composite parts, we utilized some components previously submitted by other teams. To avoid creating twins, we did not submit these parts but instead listed the original IDs for these components here.

在Composite parts的组装过程中，我们用到了一些之前队伍提交过的元件。为了避免产生twins，我们并没有提交这些部件，而是在此示出了该部件最早提交时的编号。

ID	Name	Type	Length(bp)	Introduction
Bba_K206013	loxp	Other	34	This part is the site which can be edited by Cre.
Bba_K2365044	ADH1 terminator	Terminator	488	This part is a terminator
BBa_K3183015	GSG	Protein_Domain	9	This part is used for linking our domain.

Composite parts

This section displays all the composite parts we have submitted.

这部分展出了我们用到的Composite Parts.

ID	Name	Type	Length(bp)	Introduction
Bba_K5242023	sensor_Pre	Gene expression unit	2086	This part can express RNA sensor for the pre-experiment. When gRNAs are present, they expresses blue fluorescence.
Bba_K5242024	sensor_Chk1	Gene expression unit	2403	This part can express RNA sensor for system optimization and dynamic monitoring of splice variant. When RNAs encoding Chk1 are present, they express green fluorescence.
Bba_K5242025	sensor_Chk1s	Gene expression unit	2403	This part can express RNA sensor for dynamic monitoring of splice variant . When RNAs encodingr Chk1 are present, they express green fluorescence.
Bba_K5242027	xylose-inducible component	Gene expression unit	3753	This part can transcribe different concentrations of RNA encoding Chk1 depending on the xylose concentration. We used it to create a series of RNA concentration gradients to plot a curve of editing efficiency versus target RNA concentration.
Bba_K5242033	Safety_lock_Hsp26_GSDMD	Gene expression unit	3215	This part is designed for strain security system. When RNAs encoding Hsp26 are absent, this part will cause the yeast to commit suicide. And is is the most likely design to succeed.
Bba_K5242034	Safety_lock_Hsp26_Bax20	Gene expression unit	2906	This part is designed for strain security system. When RNAs encoding Hsp26 are absent, this part will cause the yeast to commit suicide.
Bba_K5242035	Safety_lock_Hsp26_Baxmyc	Gene expression unit	3008	This part is designed for strain security system. When RNAs encoding Hsp26 are absent, this part will cause the yeast to commit suicide.
Bba_K5242036	Safety_lock_GLC3_GSDMD	Gene expression unit	3215	This part is designed for strain security system. When RNAs encoding GLC3 are absent, this part will cause the yeast to commit suicide.
Bba_K5242037	Safety_lock_GLC3_Bax20	Gene expression unit	2906	This part is designed for strain security system. When RNAs encoding GLC3 are absent, this part will cause the yeast to commit suicide.
Bba_K5242038	Safety_lock_GLC3_Baxmyc	Gene expression unit	3008	This part is designed for strain security system. When RNAs encoding GLC3 are absent, this part will cause the yeast to commit suicide.