Our project aims to engineer Pseudomonas fluorescens so that it could grow from creatinine as a renewable resource. We also took the challenge to improve Pseudomonas fluorescens natural properties to help plant growth on regolith. With BioMoon we improved this strain to make it able to grow on creatinine, a compound of urine, not yet recycled. If you want to know more, click below.

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Our team combined molecular biology, metabolic engineering and microbiology to construct and characterize our BioMoon strain, and to test its influence on plant growth on a Moon regolith simulant. Notably, we succeeded in implementing the creatinine pathway into Pseudomonas fluorescens. If you want to know more about our parts, click below.

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Our modeling strategy was key to our engineering success. We adapted a detailed in silico model of Pseudomonas fluorescens, incorporating pathways for creatinine degradation, nitrate production, and stress resistance. This model allowed us to optimize the bacterium’s performance, accelerating our bioengineering efforts.

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Spreading information about science and biology, especially to youngsters, was one of the challenges that our team has taken up. We participated in events and organized workshops for middle school and high school students to promote science, and raise interest in synthetic biology and microbiology.

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Special attention was dedicated to designing BioMoon as a marketable product. We conducted an exhaustive market analysis, identified key stakeholders, defined impacts of our BioMoon products, and built a business model with clear milestones and deliverables.

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