Safety form
A key aspect of our team's work is ensuring that every future doctor, researcher, and pharmacist learns the importance of paying close attention to both personal and environmental hygiene during complex research projects. A single poor decision in the lab or a momentary lapse in focus can easily lead to major complications. Additionally, in today's world, the significance of data safety cannot be emphasized enough. To successfully overcome these challenges, it is essential to go over each safety measure step by step, ensuring that we are fully protected in every aspect. Thorough preparation can be considered half the success in a long-term series of experiments.
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Environmental safety
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We collected all the chemicals' Safety Data Sheets in paper and digital form. These are available at the lab
room and also are at hand of the adult staff in charge.
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Bacteria that have an impact on the environment were neutralised using autoclave and bleech. The destroyed
bacteria and all used liquids are sent to a local company who deals with dangerous luqied and solid wastes.
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Personal safety
Before the lab exercises, we attended fire and accident prevention training, where the following things were
highlighted:
- Frequent hand-washing
- Frequent glove changing
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Before leaving the lab:
- No touching if the gloves are still on
- Identify the location of safety equipments
- No eating, drinking in the lab
- No working alone
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Proper dressing: long pants, closed-toed shoes, tied back long hair, no jewellery, no personal belongings
(bags, coats, etc.)
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Personal protective equipments:
- goggles/safety glasses, chemical resistance safety goggles, full face shield
- lab coats (buttoned!) - chemical apron (if you’re working with splash hazards)
- gloves – non-absorptive, thermal and puncture resistant gloves (handling hot or cold materials)
- If a hazard was detected, we could turn to the lab supervisor, teacher Andrea Borbola, for help
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Data safety
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As part of our education project, we created a video game that was sent out to several schools. Players were
asked to give no personal information, only the name of their school and their grade
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In the photos taken during the lectures we gave as part of our educational project, we took special care to
ensure that the student’s faces were not included in the photos, for reasons of privacy.
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During the Human Exercises, we asked netizens in anonymous questionnaires about their experiences within their
diseases. Only those over 18 years of age were allowed to complete the questionnaire; those under 18 years of
age needed parental permission to complete the questionnaire.
The following chemicals have been used:
THERMOFISHER-SCIENTIFIC
- Dynabeads™ MyOne™ Streptavidin C1 10mg/mL
- UltraPure™ 1 M Tris HCl-oldat pH=7.50
- EDTA (0.5 M), pH 8.0, RNase-free
- NaCl (5 M), RNase-free
- DEPC-Treated Water
- RNaseZap™ RNase Decontamination Solution
- GeneJET RNA Purification Kit
- GeneJET PCR Purification Kit
- Rapid DNA Ligation Kit
- GeneJET Plasmid Miniprep Kit
- GeneJET Plasmid Maxiprep Kit
- Na-acetát 3M (Rnase-free) pH=5.50
- FastDigest EcoRI
- FastDigest BglII
- FastDigest BamHI
- FastDigest SalI
- TBE (10X), RNase-free oldat
- TBE (10X), RNase-free oldat
- Mouse anti-6x-His Epitope Tag Monoclonal Antibody (HIS.H8)
- Pierce™ 10X Western Blot Transfer Buffer, Methanol-free
- HeLa Lysate
- Accessory Proteins Green
- Reaction Mix for IVT
- 5X Dialysis Buffer
- Positive Control DNA: pCFE-GFP (0.5µg/µL, 10µg)
- pT7CFE1-NHis-GST-CHA plasmid
- Primary antibody (anti-DAO mouse monoclonal), lyophilized
- Primary antibody (anti-HNMT mouse monoclonal), lyophilized
- Ethidium-bromide
- Copper sulphate (in very low concentration)
BIO-RAD:
- Laemmli sample buffer
- Precision Plus Protein Kaleidoscope prestained standards
- 10x Tris-glycine-SDS electrophoresis buffer
- Bio-Safe™ Coomassie stain for proteins
- Actin & myosin standard – 500 µg lyophilized
- DTT (DL-Dithiothreitol)
- Low-melt agarose
- Acetic acid
- Reagent alcohol/ethanol
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Secondary antibody (goat anti-mouse polyclonal antibody conjugated to horseradish peroxidase (HRP), lyophilized)
- HRP color detection reagent A
- HRP color detection reagent B
- 10x Tris-glycine
- Non-fat dry milk blocker
- 10x phosphate buffered saline (PBS)
- 10% Tween 20
- Nitrocellulose sheets, 0.45 µm
- Blotting paper sheets
IDT-DNA:
- Cy5 (Cyanine5) for the aptamer reactions
- BHQ (Black-Hole-Quencher) for the aptamer reactions
- Biotin