Contribution
↓Team TERMOSZ-Selye-HUN 2024 designed the codon optimisation of human Histamine-N-Methlytransferase (HNMT) and rat Diamine Oxidase (DAO) genes for the e. coli chassis, and experimented to evaluate whether this had impacted the production or the function of the proteins. In the experiments, the team utilised the E. coli strain DH10B.
Refer to the basis of our contributions here:
Refer to our parts and further characterization here:
Through our project, we aimed to create a valuable resource for future iGEM teams, ensuring that our work could serve as a lasting contribution. To achieve this, we collaborated with four other iGEM teams—Bielefeld-CeBiTec, Linköping, Patras, and Radboud University—to
produce a handbook on lipid delivery systems. This handbook offers essential insights, including general information
about lipid delivery systems, how each team used them in their projects, and detailed protocols. Additionally, we’ve
included practical tips, such as time and cost estimations to assist future teams in planning and executing their
experiments. The PDF version of the handbook is available in the Human Practices section of our team's wiki.
https://2024.igem.wiki/termosz-selye-hun/human-practices
Furthermore, in the education part of our project, we developed an innovative LEGO DNA model with the assistance of Dániel Boncz. This model is specifically designed to make the concepts of DNA structure and function more accessible to younger audiences. Using this interactive approach, children can build the two strands of DNA, as well as the mRNA by using the rules of base pairing. The codon table allows them to determine the sequence of amino acids in the protein to be synthesised. This model visualises and grasps the process of transcription and translation, while the playful nature of this method makes it an excellent tool for teaching complex biological concepts in a simplified way.
For those interested in building the LEGO DNA model, we have provided a list of part ID numbers, the exact quantities of each piece, and detailed assembly instructions in the Education section of our team's wiki.
https://2024.igem.wiki/termosz-selye-hun/education
In the education part of our project, we developed an innovative LEGO DNA
model with the assistance of Dániel Boncz. This model is specifically designed
to make the concepts of DNA structure and function more accessible to younger audiences
paying particular attention to not to use green pieces to ensure that students with red-green
color blindness could also participate. For those interested in building the LEGO DNA model, we
have provided a list of part ID numbers, the exact quantities of each piece, and detailed assembly
instructions in the Education section of our team's wiki:
In the education part of our project we also developed a video game to make the field of synthetic biology understandable and appealing to high school students. The game promotes synthetic biology, particularly among students who are on the verge of choosing their career paths. Those who want to try out our video game or get inspiration from it, it can be accessed in the Education section of our team's wiki:
https://2024.igem.wiki/termosz-selye-hun/education
We created a questionnaire to assess patients' itching habits and possible needs for a medicine. In our questionnaire, we linked the occurrence of local itching to patients' diet and chronic diseases, so we could get specific feedback from patients and take a more targeted approach to the management of their symptoms. The questionnaire can be accessed in the Human Practices section of our team's wiki:
SPAT is a universal tool which aims to measure the sustainability of companies in the pharma-cosmetic industry. The survey assesses sustainability practices in eight factors, with 5 questions in each factor. After the evaluation of the answers, the results can be displayed on a radial diagram for analysis and comparison. The SPAT can be accessed in the Sustainable Development section of our team's wiki: