ALIVE
synthetic biology for wastewater treatment
From these, metabolites can be released into the environment, being introduced into surface and ground water
Some of them can be treated adequately in wastewater treatment facilities
How can we change this?
Using laccase
An enzyme that can reduce EE2 solubility, making it easier to remove it from water
It is found in a fungus that produces humic acid when stressed
This acid could be harmful for skin cells
Thus, a new organism must be selected
Choosing the right chassis
Between all candidates we have nominated
Chlorella vulgaris
For its different abilities
However
Adding laccase into Chlorella could lead to its overproduction and potentially destroy the system
A new protein must be made
Regulating production
A protein that can tell the system to produce laccase only in the presence of EE2
