Overview
2023.12.3
Team Formation
We set up a team with 12 members.
2024.12.16-2024.2.6
Experimental Skills and Literature Review Training
2024.1.16-2024.3.16
Brainstorming
Brainstorming sessions were conducted in groups, with each group working on independent projects. Feasibility assessments led to the elimination of impractical proposals during meetings.2023.3.20
Project Identification
The interest in our project was piqued by a sugar content visualization display in a hospital, an encounter that occurred when one of our members was admitted due to irregular dietary habits.2024.3.16-2024.4.1
Background Research and Preliminary Experiments
The preliminary design of the entire project system was completed, along with extensive background and literature research. Preliminary experiments were initiated.
2024.5.12-2024.9.18
Mid-term Experiments and Product Iteration
2024.9.19-2024.10.1
Completion of Experiments and Wiki Upload
All experiments were completed and the results were uploaded to our wiki.2024.10.23
iGEM Jamboree
Participation in the iGEM Jamboree, an event showcasing our project to the synthetic biology community.Team log
2024.1.16-2024.3.10 Brainstrom
1.16
Start Our Brainstorming
1. Discussed and determined the complete process of team brainstorming and established the "three-steps" strategy:Step 1. Release your thoughts and record them.
Step 2. Search and organize a lot of data.
Step 3. Initially select the project with the most development potential for further advancement, and finally select the most suitable one as our project after reviewing each pre-project from multiple perspectives.
2. Created a brainstorming summary sheet to record our interesting ideas.
1.16-3.10
Some Brainstorming was Constructed
Climate Crisis:
Jiachun Chen and Yuanrun Liu —— Methanol bioabsorption pigmentQizheng Liu, Zhengyue Hong and Haosheng Deng —— Biological feed to reduce carbon monoxide emissions from livestock
Qizheng Liu and Zhengyue Hong —— Quorum quenching method for treating water blooms
Bioremediation:
Kexin Li and Siyuan Guo —— Bioremediation of petroleum-contaminated soilHaosheng Deng —— Bioremediation of heavy metal pollution
Yuntian Wu —— Bioremediation of cultural relics and ancient buildings
Agriculture:
Haosheng Deng —— Research and development of targeted fertilizers for multi-fruit eggplant treesFood and Nutrition:
Qizheng Liu and Zhengyue Hong —— Sugar substitute fruitsFashion and Cosmetics:
Zejia Wei —— Biological production of carmine cochineaDiagnostics:
Yitong Li —— Biological detection of parvovirus in puppiesJiachun Chen —— Intestinal colonizing flora to improve obesity
Ruohan Chen —— Using antibodies to reduce drug addiction in people
Therapeutics:
Zhengyue Hong and Zejia Wei —— Hydrogel for treating dental caries and dental diseasesYuntian Wu —— Biological glue instead of medical tension reducer
Yitong Li —— Mass production of albumin
Biomanufacturing:
Yitong Li —— Biological moisture-proof pigmentYuanrun Liu —— Biological smoke-proof wall
Qizheng Liu, Zhengyue Hong and Guangbin An —— Biological production of badminton feathers.
2024.3.10
Project Selection from Brainstorming Session
We have selected three projects for further exploration:
1. Production of Sugar Substitute Fruits: Utilizing grapes as a biological chassis, we aim to engineer a reduction in the production of free sugars and to enhance the production of natural sugar substitutes.2. Fruit Preservation: We plan to modify antagonistic yeasts to secrete a gelatinous substance that can be sprayed onto fruits to serve as a preservative.
3. Production of Carmine Red: We intend to harness microbial fermentation for the production of carmine red pigment.
2024.3.12
Changes in Brainstorming
When one of our members was hospitalized due to irregular diet and lifestyle, the display cabinet visualizing the sugar content of beverages in the hospital aroused our interest.2024.3.14
Project Confirmation:
After consulting with our Principal Investigator (PI) and advisors, we commenced research on the production of sugar substitutes.
2024.3.16-2024.9
Wet Lab
3.16-3.30
Initial Phase Attempts
Prokaryotic Expression Vector Construction and Transformation into E. coli; Detection of Prokaryotic System Expression Levels:
3.16
Yitong Li —— Retrieved E. coli BL21 from the -80°C freezer and inoculated it onto solid culture media for overnight incubation.3.18
Yitong Li —— Observed the growth status of E. coli.3.19
Yitong Li —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.3.22
Yitong Li —— Prepared competent E. coli, transformed the constructed plasmid into E. coli, and then inoculated it into antibiotic-containing culture media for selection and cultivation.3.20
Yitong Li —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.3.23-3.30
Yitong Li —— Performed colony PCR and agarose gel electrophoresis to detect E. coli, and used Western Blot (WB) to examine the expression of the target protein in E. coli.3.31-6.6
Second Phase
Construction of Eukaryotic Expression Plasmids, Transformation into Tomato Plants via Viral Methods; Detection of Plant Expression Levels:
Cultivation of the First Batch of Tomato Plants
3.31
Siyuan Guo, Yitong Li, Guangbin An, Qizheng Liu —— Cleaned and disinfected the seeds of the first batch of plants, then sowed them into nutrient soil.4.1-4.6
Yitong Li, Guangbin An —— Continuously observed and recorded the germination of the seeds, and transplanted the successfully germinated seedlings into individual pots.4.7-4.14
Yitong Li, Guangbin An —— Continuously observed and recorded the growth of the plants, applying fertilizer and pesticides as appropriate.4.15-6.18
Siyuan Guo, Yitong Li, Guangbin An, Qizheng Liu —— Continuously observed and recorded the growth of the plants, applying fertilizer and pesticides as appropriate.Agrobacterium Cultivation
4.1-4.3
Siyuan Guo, Qizheng Liu —— Retrieved Agrobacterium GV3101 from the -80°C freezer, activated it, and inoculated it onto solid culture media for overnight incubation.4.4
Siyuan Guo, Qizheng Liu —— Observed the growth status of Agrobacterium.4.5
Siyuan Guo, Qizheng Liu —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.4.6
Siyuan Guo, Qizheng Liu —— Prepared competent Agrobacterium, transformed the constructed plasmid into Agrobacterium, and then inoculated it into antibiotic-containing culture media for selection and cultivation.4.10
Siyuan Guo, Qizheng Liu —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.4.12
Siyuan Guo, Qizheng Liu —— Performed colony PCR and agarose gel electrophoresis to detect Agrobacterium, confirming the success of the transformation.Infection and Detection of Plants
4.14
Siyuan Guo, Qizheng Liu —— Infected the first batch of tomato plants with Agrobacterium using leaf infiltration.4.28
Siyuan Guo, Qizheng Liu —— Collected leaves from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.4.29
Siyuan Guo, Qizheng Liu —— Extracted protein samples from the plant leaf samples, and stored the remaining samples at -80°C.4.30
Siyuan Guo, Qizheng Liu —— Performed PCR detection on the leaf tissue samples.5.1-5.2
Siyuan Guo, Qizheng Liu —— Performed Western Blot (WB) detection on the extracted leaf proteins.5.14
Siyuan Guo, Qizheng Liu —— Collected flowers from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.5.15
Siyuan Guo, Qizheng Liu —— Extracted protein samples from the plant flower samples, and stored the remaining samples at -80°C.5.16
Siyuan Guo, Qizheng Liu —— Performed PCR detection on the flower tissue samples.5.17-5.18
Siyuan Guo, Qizheng Liu —— Performed Western Blot (WB) detection on the extracted flower proteins.6.14
Siyuan Guo, Qizheng Liu —— Collected mature fruits from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.6.15
Siyuan Guo, Qizheng Liu —— Extracted protein samples from the plant fruit samples, and stored the remaining samples at -80°C.6.16
Siyuan Guo, Qizheng Liu —— Performed PCR detection on the fruit tissue samples.6.17-6.18
Siyuan Guo, Qizheng Liu —— Performed Western Blot (WB) detection on the extracted fruit proteins.3.31-9.20
Third Phase
Conduct of Transgenic Experiments
Cultivation of the Second Batch of Tomato Plants
4.31
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Cleaned and disinfected the seeds of the second batch of plants, then sowed them into nutrient soil.5.1-5.8
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Observed and recorded the growth conditions of the plants, applying fertilizer and pesticides as appropriate.Agrobacterium Cultivation
5.3
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Retrieved Agrobacterium GV3101 from the -80°C freezer, activated it, and inoculated it onto solid culture media for overnight incubation.5.4
Siyuan Guo, Qizheng Liu —— Observed the growth status of Agrobacterium.5.5
Siyuan Guo, Qizheng Liu —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.5.6
Siyuan Guo, Qizheng Liu —— Prepared competent Agrobacterium, transformed the constructed pBWA(V)HS and pCAMBIA1301 plasmids into different groups of Agrobacterium, and then inoculated them into antibiotic-containing culture media for selection and cultivation.5.10
Siyuan Guo, Qizheng Liu —— Selected appropriate single colonies and inoculated them into liquid culture media for scaled-up cultivation.5.12
Siyuan Guo, Qizheng Liu —— Performed colony PCR and agarose gel electrophoresis to detect Agrobacterium, verifying the success of the transformation.Infection and Detection of Plants and Subsequent Experiments
5.9-5.12
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— After the leaves fully expanded, excised the hypocotyl and cotyledon tips with a scalpel, leaving the middle part to be cut into 2 to 3 segments and inoculated into pre-cultivation medium.5.13-5.15
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Infected the explants with the transformed Agrobacterium and then co-cultivated.5.16-6.1
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Transferred the callus tissue from the recovery culture of the second batch of plant explants to the selection medium for cultivation.6.2-7.2
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— After selecting suitable callus tissue, inoculated it onto the differentiation medium for cultivation.7.3-7.14
Siyuan Guo, Qizheng Liu, Yitong Li, Guangbin An —— Transferred the differentiated seedlings to the rooting medium for cultivation.7.15-7.26
Qizheng Liu, Yitong Li, Guangbin An —— Transplanted the seedlings into water for cultivation and continuously observed and recorded their growth.7.27
Qizheng Liu, Yitong Li, Guangbin An —— Transplanted the plants into soil for cultivation and continuously observed and recorded their growth.8.10
Qizheng Liu, Yitong Li, Guangbin An —— Collected leaves from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.8.11
Qizheng Liu, Yitong Li, Guangbin An —— Extracted protein from the plant leaf samples, and stored the remaining samples at -80°C.8.12
Qizheng Liu, Yitong Li, Guangbin An —— Performed PCR testing on the leaf tissue samples.8.13-8.14
Qizheng Liu, Yitong Li, Guangbin An —— Performed Western Blot (WB) testing on the extracted leaf proteins.8.22
Qizheng Liu, Yitong Li, Guangbin An —— Collected flowers from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.8.23
Siyuan Guo, Qizheng Liu —— Extracted protein from the plant flower samples, and stored the remaining samples at -80°C.8.24
Qizheng Liu, Yitong Li, Guangbin An —— Performed PCR testing on the flower tissue samples.8.24-8.25
Qizheng Liu, Yitong Li, Guangbin An —— Performed Western Blot (WB) testing on the extracted flower proteins.9.5
Qizheng Liu, Yitong Li, Guangbin An —— Collected mature fruits from the first batch of plants and quickly froze them with liquid nitrogen, then crushed them.9.5
Qizheng Liu, Yitong Li, Guangbin An —— Extracted protein from the plant fruit samples, and stored the remaining samples at -80°C.9.6
Qizheng Liu, Yitong Li, Guangbin An —— Performed PCR testing on the fruit tissue samples.9.7-9.8
Qizheng Liu, Yitong Li, Guangbin An —— Performed Western Blot (WB) testing on the extracted fruit proteins.9.10
Guangbin An —— Used the Thaumatin ELISA kit to measure the concentration of sweet protein Thaumatin in the experimental group of tomatoes, achieving the expected results.9.11
Guangbin An —— Detected the concentration of citric acid and glucose in the transgenic tomato fruits using colorimetric assay kits.9.12
Qizheng Liu, Guangbin An —— Used a combination of human sweet receptor protein T1R2 and HA tag ELISA kits to determine whether the sweet protein in the transgenic tomatoes could interact with the human sweet receptor protein T1R2.9.14-9.18
Yuanrun Liu, Guangbin An —— Used the control group of tomato fruits to establish a database by adding a concentration gradient of Thaumatin sweet protein.9.18-9.26
Yuanrun Liu —— Used the established database to test the sweetness of the experimental group of tomato samples and achieved the expected results.9.14-9.20
Yitong Li, Qizheng Liu —— Constructed the pGD_SPS-NTPP-Thaumatin-EGFP plasmid.9.21-9.24
Yitong Li, Qizheng Liu —— The constructed pGD plasmid was transformed into Agrobacterium.9.25-9.29
Yitong Li, Qizheng Liu —— The aforementioned Agrobacterium was used to infect the leaves of commercially available tobacco and wild-type tomatoes.9.30
Yitong Li, Qizheng Liu —— The tobacco and tomato leaves that had been infected were observed under a laser scanning confocal microscope.Thaumatin sweet protein.
9.9-9.23
Fourth Phase
Conduct of Vacuolar Localization Experiments:
9.9
Yitong Li, Guangbin An, Qizheng Liu —— Retrieved Agrobacterium GV3101 from the -80°C freezer and inoculated it onto solid culture media for shaking culture for 3 hours, then transferred it to liquid culture media for overnight cultivation.9.9-9.10
Yitong Li, Guangbin An, Qizheng Liu —— Constructed a plasmid pGD carrying a vacuolar targeting peptide and transformed it into competent Agrobacterium GV3101 , then inoculated it onto solid culture media containing resistance.9.12
Yitong Li, Guangbin An, Qizheng Liu —— Selected appropriate single colonies and inoculated them into liquid culture media for further cultivation.9.14
Yitong Li, Guangbin An, Qizheng Liu —— Infected commercial Nicotiana benthamiana plants with four leaves using the aforementioned Agrobacterium.9.23
Yitong Li, Guangbin An, Qizheng Liu —— Performed confocal microscopy observation on Nicotiana benthamiana leaves.Products
8.14
All team members —— After a team meeting, we considered that our initial product was a sweet tomato, which, however, had several drawbacks, such as inconvenience for carrying, transportation, and utilization. Considering our original intention was to produce a sugar substitute, our initial idea was to provide our sugar substitute product to factories for the replacement of sugar at the source.8.20
Yitong Li, Ruohan Chen, Kexin Li —— After visiting the CHI Forest factory, we learned about the compressed form of sugar substitute materials they use, which inspired us. Upon returning and discussing, we decided to design our product as a concentrated liquid form.9.4
Jiachun Chen, Zejia Wei, Kexin Li —— We visited a food exhibition held in our city and noticed that many vendors were selling products in very small packaging. They mentioned this was to allow customers to easily try or carry the products. Considering simplicity and cost-effectiveness, after obtaining the fruit from the tomato plants, we wished to use a simple procedure to experiment with the manufacture of the product. Therefore, our final strategy was to use concentrated tomato fruit juice as a sweetener, enclosed in a small packet that is convenient for carrying and adding.9.20
Siyuan Guo —— Designed the cover for the product's small packaging.Dry Lab
6.25-7.15
Ruohan Chen —— Utilized AlphaFold software for the visualization analysis of Thaumatin protein, Brazzein protein, and the human sweet receptor protein T1R2.7.16-8.1
Ruohan Chen —— Predicted the binding models of the human sweet receptor protein and sweet proteins (Protein-Protein Interaction, PPI).8.2-8.20
Ruohan Chen —— Identified the wilting peptides carried by Thaumatin and analyzed three protein sites responsible for bitterness.8.21-9.5
Ruohan Chen —— Employed computational algorithms to perform random mutations based on these three sites, simulating the process of directed evolution.9.6-9.23
Ruohan Chen —— Utilized the mutated Thaumatin and reapplied the PPI protein interaction principle for analysis within the computer, and selected the optimal sweet protein sequence.Hardware
4.25-5.15
Haosheng Deng —— After reviewing extensive literature, proposed the preliminary concept of a bio-electronic detection device for detecting sweet proteins and regular sugars.5.16-6.10
Haosheng Deng —— Constructed a specific model, but after discussion with our Principal Investigator (PI), it was concluded that the bio-electronic detection device still had many unavoidable drawbacks. Therefore, Haosheng Deng abandoned the experiments related to this device and began researching other detection hardware related to sugar detection.6.11-7.20
Yuanrun Liu, Haosheng Deng —— Studied the specific mechanisms and usage methods of commercial electronic tongues.7.22-7.26
Haosheng Deng —— In the process of integrating human practices, he discovered that current detections of free sugars are all one-to-one detection methods, which gave Haosheng Deng great inspiration.7.26-8.10
Haosheng Deng —— After reviewing a vast amount of literature, Haosheng Deng proposed SweetGlow, a common sugar detector based on lanthanide metal-ligand fluorescence, hoping to detect three or more types of sugars within ten minutes and accurately measure their concentrations. Recognizing the substantial workload required to observe the effects of protein detection directly, Haosheng Deng proposed the concept of a lateral flow immunoassay strip for detecting the concentration of sweet proteins in our tomato plants. After team discussion, we began working on the conceptual model design and principle verification of SweetGlow.8.11-9.20
Haosheng Deng —— Using 3D printing, successfully created a conceptual model of SweetGlow. After contacting Professor Yujuan Chai, an expert in lateral flow immunoassay technology, and with Professor Chai's guidance and assistance, began to develop a lateral flow immunoassay strip for detecting thaumatin, and successfully detected it, creating a specific hardware model. Successfully produced the prototype of SweetGlow.Human Practices
2023.12.27
Zejia Wei, Siyuan Guo, Yuanrun Liu, Guangbin An, Haosheng Deng —— Discussed synthetic biology and the use of cell factories with Xbiome company. This meeting inspired the idea of using cell factories for our project.2024.1.26
Zejia Wei —— The new team SMU-GDMU-China visited Shenzhen University to learn and gain experience.2024.1.29
Zejia Wei —— Returned to her high school alma mater, the High School Affiliated to SUSTech (HSAS), to give a presentation. At the meeting, she introduced iGEM and encouraged interested students to contact her for further information.2.27
All team members —— Successfully held an iGEM competition exchange meeting, inviting Vice Chairman of the iGEM Organizing Committee, Miss Zhang Nan, and teams from Southern University of Science and Technology (SUSTech), South China University of Technology (SCUT), Beijing Normal University Zhuhai Campus (BNUZH), Shenzhen Polytechnic University (SZPU), Southern Medical University (SMU), and Guangdong Medical University (GDMU).3.31
Zejia Wei, Yuanrun Liu, Qizheng Liu, Jiachun Chen —— The iGEM teams from the University of Macau and Beijing Normal University, Zhuhai Campus, came to Shenzhen University to exchange the outcomes of brainstorming sessions.4.12
All team members —— Hosted students from the Yantian District Committee. We introduced synthetic biology in layman's terms to the students and led them an activity for creatively designing synthetic biology projects.4.26
Team members Zejia Wei, Qizheng Liu, Zhengyue Hong, Haosheng Deng, Yuntian Wu, Guangbin An —— Produced a safety propaganda video and posted it on a video platform, using an entertaining approach to educate the public about laboratory safety.5.18
All team members —— Organized the 8th iGEM Southern China Regional Meeting. The meeting brought together teams from the South China region, providing a platform for interactive displays, collaborative communication, and mutual learning, fostering an atmosphere conducive to exploring synthetic biology.6.8
Team members Zejia Wei, Jiachun Chen, Qizheng Liu, Zhengyue Hong, Yitong Li, Guangbin An, Ruohan Chen, Yuanrun Liu —— During the project advancement phase, we met with the team leader from CAU-China to discuss some issues related to the project.6.11
Zejia Wei, Yuntian Wu, Kexin Li, Qizheng Liu —— Conducted a street interview activity to gather public opinions on sugar substitutes and genetically modified foods.6.14
Zejia Wei, Kexin Li, Zhengyue Hong, Yuntian Wu —— Visited the Futian Foreign Language School to give a biology lecture, introducing basic biological knowledge to students and sparking their interest in biology.6.22
All team members —— Opened our laboratory to the Shenzhen community for a visit, teaching citizens to identify laboratory equipment, learn how to use basic laboratory equipment such as pipettes, and popularize knowledge about synthetic biology.6.23
Zejia Wei, Zhengyue Hong, Haosheng Deng —— Exchanged offline with students from HKUST-GZ, lending them a DNA reagent.7.9
All team members —— Held an online meeting with AIS-China to exchange ideas and solve mutual doubts.7.12
All team members —— Attended the CCiC meeting, which brought together teams from different regions, providing a platform for interactive displays, collaborative communication, and mutual learning.7.18
Siyuan Guo, Yuanrun Liu —— Provided opportunities for children in remote areas to learn about advanced knowledge, introduced them to synthetic biology, and conducted several simple experiments.7.18
All team members —— Held an offline meeting with members of BASIS-China to discuss experimental design and HP.7.19
Kexin Li, Yuntian Wu —— Consulted Professor Tengbo Huang on issues related to sugar storage in tomatoes.7.22
Yuanrun Liu, Siyuan Guo, Qizheng Liu, Zhengyue Hong —— Several high school leagues came to Shenzhen University to exchange ideas on project design with us.7.22
Yuanrun Liu, Yuntian Wu —— Attended a plant science lecture at Shenzhen University, learning about organelle localization.7.23-7.24
Zejia Wei, Kexin Li, Yuntian Wu, Ruohan Chen, Haosheng Deng —— Visited a large number of manufacturers related to sugar substitutes to explore the urgent problems that need to be solved in the sugar substitute market.7.25-7.26
Zejia Wei, Kexin Li, Yuntian Wu, Ruohan Chen, Haosheng Deng —— Visited large planting sites to understand the current issues in plant cultivation.7.27
Zejia Wei, Yuntian Wu —— Participated in Synbio Talk organized by CityU as a guest speaker, which popularized the knowledge of synthetic biology to high school students and emphasized safety issues.7.28
Zhengyue Hong, Qizheng Liu, Kexin Li, Yuntian Wu —— Consulted Professor Mo on the feasibility of tomato chassis and received a positive response.7.30
Zejia Wei, Yuntian Wu —— Shared our project with Professor Liu Yun from Shenzhen University, who pointed out issues related to the gentamicin gene, which drew our attention.7.31
Kexin Li, Yitong Li —— Inquired about issues related to genetically modified food and diabetes, which increased our confidence in the feasibility of sugar substitutes.8.1 - 8.3
Zejia Wei, Kexin Li, Yuntian Wu —— Attended the 2nd iGEM Greater Bay Area Synthetic Biology Industry-Academia-Research Forum, exploring new ways of cooperation among iGEM teams, and emphasizing cooperation between academia and industry.8.2
Zejia Wei, Yuntian Wu —— Visited the Shenzhen Guangming New District Synthetic Biology Industrial Park for study and learning. Had a brief conversation with the heads of some synbio companies, understanding the importance of food safety in synthetic biology.8.2
Zejia Wei, Kexin Li, Yuntian Wu —— Collaborated with university teams such as Jilin University to write a white paper. We were responsible for the interpretation and analysis of domestic and international laws on synthetic biology food.8.5
Kexin Li, Yuntian Wu —— To define the safety of genetically modified food and ensure the stability of proteins, we consulted expert Liqing Zhao.8.15
Zejia Wei, Yuntian Wu —— Inquired about the market launch process for synthetic biology food in Shenzhen from the Shenzhen Market Supervision Administration.8.17
Kexin Li, Yuntian Wu —— Discussed safety issues with the iGEM team from Hubei University.8.19
Zejia Wei, Yuntian Wu —— Hosted youth from all over the world who came to Shenzhen University to study, and explained the development history of synthetic biology to them.8.20
Kexin Li, Ruohan Chen, Yitong Li —— Visited the CHI Forest Factory in Zhaoqing to understand the company's use of sugar substitutes.8.21-8.22
Zejia Wei, Yuntian Wu, Yuanrun Liu, Qizheng Liu, Zhengyue Hong, Jiachun Chen —— Attended the Asia-Pacific Mini Jamboree at the University of Hong Kong.8.23
Haosheng Deng, Yuanrun Liu —— Had a brief discussion with the electronic tongue business.8.27
Zejia Wei, Jiachun Chen, Kexin Li —— Visited the Shenzhen Agricultural Promotion Center and talked with Chief Researcher Ms. Li about tomato-related issues.8.31
Zejia Wei, Ruohan Chen, Qizheng Liu —— Participated in the biosafety propaganda activity organized by the Southern University of Science and Technology (SUSTech), popularizing the safety of sugar substitutes for local residents.9.2
Zejia Wei, Yuntian Wu —— Introduced iGEM to the freshmen of the Collage of Life Sciences and Oceanography, Shenzhen University.9.4
Zejia Wei, Kexin Li, Jiachun Chen —— Attended the SIAL Food Exhibition to understand how other food companies view sugar substitutes and synthetic biology food.9.6
Jiachun Chen, Kexin Li, Yuntian Wu, Yuanrun Liu, Guangbin An, Zhengyue Hong, Yitong Li —— Visited the Shenzhen University Longhua Transgenic Base to learn about the cultivation standards and restrictions of genetically modified crops.9.13
All team members —— Promoted iGEM at the school's club carnival.Art Desigining
4.10-5.10
Siyuan Guo —— Designed the venue and layout for the 8th iGEM Southern China Regional Meeting, designed the team uniform styles, and created patterns for peripheral gift items.5.11-5.19
Siyuan Guo —— Designed the logo for the 2024 SZU-China team.5.20-6.24
Siyuan Guo, Kexin Li, DingCheng Qi —— Produced a summary video for the 8th iGEM Southern China Regional Meeting.6.25-7.15
Siyuan Guo, Kexin Li, Jiachun Chen, Guangbin An, Yitong Li, Qizheng Liu —— Designed posters, PPTs, and promotional products for the CCiC Exchange Meeting.7.16-9.30
Siyuan Guo, Kexin Li —— Designed patterns and graphics for the wiki webpage.8.10-9.6
Siyuan Guo —— Created a propaganda picture book about sugar substitutes.8.15-9.4
Kexin Li, Siyuan Guo, DingCheng Qi —— Provided illustrations or animations for the promotional video and edited the promotional video.10.3-10.9
Kexin Li, Siyuan Guo —— Designed illustrations and special effects for the team's presentation video.DingCheng Qi —— Edited the presentation video.
Wiki Develop
6.28-7.15
Zhengyue Hong — Familiarized with the official iGEM Wiki editing operations.7.15-8.25
Zhengyue Hong — Learned HTML, CSS, and JavaScript.8.26-9.1
Zhengyue Hong — Constructed the Wiki framework for our SZU iGEM 2024.9.2-10.2
Zhengyue Hong — Completed the homepage and gradually finished other necessary pages.With Deepest Appreciation:
We acknowledge the assistance of advisors. We are also very thankful for the other members of the SZU 2024 iGEM team and our close collaboration, which has enabled us to successfully complete the creation of this Wiki!
