The PCR Reaction of DNA
PCR product Purification
Restriction Endonuclease Enzymatic Shearing of DNA
Gibson assemble
Golden Gate assemble
1% Agarose Gel Electrophoresis
Plasmids extraction
DNA transformation of *E. coli*
Overnight Bacterial Culture
Colony PCR
Overnight Bacterial Culture and Protein Induction
Tagatose synthesis
Fermentation
Tagatose concentration determination
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