Microorganism safety
Handling bacteria, particularly those classified under Risk Group 2, such as Salmonella and Staphylococcus aureus used in our project, requires stringent safety protocols to protect laboratory personnel and prevent contamination. Below document outlines the safety procedures and precautions necessary for safely conducting experiments involving Escherichia coli, Salmonella, and Staphylococcus aureus.
Specific Procedures for Handling Pathogenic Bacteria
1. Escherichia coli (E. coli):
Culture Handling: Use aseptic techniques to transfer and manipulate cultures. Work inside a BSC to minimize exposure.
Incubation: Incubate cultures in sealed containers or flasks with vented caps to prevent aerosol escape.
Spill Response: In the event of a spill, cover the area with paper towels soaked in disinfectant (e.g., 10% bleach) and allow it to sit for at least 15 minutes before cleanup.
2. Salmonella and Staphylococcus aureus (Risk Group 2):
Culture Handling: Use BSL-2 containment practices when handling pathogenic bacteria. Work within BSCs to avoid exposure and contamination.
Waste Disposal: Autoclave all waste materials, including culture plates, pipette tips, and gloves, before disposal.
Hand Hygiene: Wash hands thoroughly with soap and water after handling Salmonella and Staphylococcus aureus cultures, even if gloves were worn.
Spill Response: In case of a spill, evacuate the area and allow aerosols to settle. Cover the spill with paper towels soaked in disinfectant (e.g., 10% bleach) and allow it to sit for at least 30 minutes before cleanup.