In order to provide a simpler, time-saving and accurate method for the detection of foodborne bacteria, we screened three phage-derived bacterial-binding protein (PBPs), i.e., T3 phage tail fiber protein (TFP) gp17, Salmonella phage CKT1 tailspike protein (TSP), and Staphylococcus aureus phage plyV12 endolysin cell wall binding domain (CBD), as the base parts. On this basis, the following composite parts were designed for the preparation of AuNP@PBP and tested for bacterial detection and photothermal sterilization. For more detailed information, please refer to the table below (Table 1).