Notebook
Day1-20240726
Task
1.Safety training
2.LB Medium preparation and autoclaving
3.Incubate bacterical strains containing plasmids
1.Safety training
2.LB Medium preparation and autoclaving
3.Incubate bacterical strains containing plasmids
Day2-20240727
Task
1.Plasmid extraction
2.Amplify target gene and vector by PCR
3.Agarose Gel Electrophoresis
Results
1.Plasmid extraction
2.Amplify target gene and vector by PCR
3.Agarose Gel Electrophoresis
Results
Day3-20240728
Task
1.DNA gel extraction
2.Gibson assembly
3.Transform competent cells (DH5α)
4.Cell plating and incubate overnight at 37°C
1.DNA gel extraction
2.Gibson assembly
3.Transform competent cells (DH5α)
4.Cell plating and incubate overnight at 37°C
Day4-20240729
Task
1.Selection of monoclonal colonies and sub-culturing
2.Colony PCR for identifying positive colonies
3.Selection of positive colony and inoculate in 10 mL liquid LB medium
Results
1.Selection of monoclonal colonies and sub-culturing
2.Colony PCR for identifying positive colonies
3.Selection of positive colony and inoculate in 10 mL liquid LB medium
Results
Day5-20240730
Task
1.Plasmid extraction
2.Transform competent cells (BL21)
3.Selection of BL21(DE3) positive colony and inoculate in 10 mL liquid LB medium
1.Plasmid extraction
2.Transform competent cells (BL21)
3.Selection of BL21(DE3) positive colony and inoculate in 10 mL liquid LB medium
Day6-20240731
Task
1.Expansion of BL21 bacterial culture
2.Induction of protein expression with IPTG
3.Synthesis of AuNPs
4.Preparation of SDS-PAGE gel
Results
1.Expansion of BL21 bacterial culture
2.Induction of protein expression with IPTG
3.Synthesis of AuNPs
4.Preparation of SDS-PAGE gel
Results
Notes: Color Change Observed During the Synthesis of Gold Nanoparticles
Day7-20240801
Task
1.Protein extraction and purification
2.SDS-PAGE gel Electrophoresis
3.Buffer exchange of purified protein
Results
1.Protein extraction and purification
2.SDS-PAGE gel Electrophoresis
3.Buffer exchange of purified protein
Results
Day8-20240802
Task
1.AuNPs@PBP preparation and detection
2.Cultivate bacteria for subsequent detection (Escherichia coli, Salmonella enterica, Staphylococcus aureus)
3.Gradient dilution of detection bacterial
Results
1.AuNPs@PBP preparation and detection
2.Cultivate bacteria for subsequent detection (Escherichia coli, Salmonella enterica, Staphylococcus aureus)
3.Gradient dilution of detection bacterial
Results
Note: UV-Vis Absorption Spectra of AuNPs Conjugated with PBP Proteins
Day9-20240803
Task
1. Colorimetric detection of bacterial by AuNPs@PBP
Results
1. Colorimetric detection of bacterial by AuNPs@PBP
Results
Note: Colorimetric Analysis of Bacteria Detection Using AuNP@PBP Conjugates