Task
1.Safety training
2.LB Medium preparation and autoclaving
3.Incubate bacterical strains containing plasmids

Task
1.Plasmid extraction
2.Amplify target gene and vector by PCR
3.Agarose Gel Electrophoresis
Results

Task
1.DNA gel extraction
2.Gibson assembly
3.Transform competent cells (DH5α)
4.Cell plating and incubate overnight at 37°C

Task
1.Selection of monoclonal colonies and sub-culturing
2.Colony PCR for identifying positive colonies
3.Selection of positive colony and inoculate in 10 mL liquid LB medium
Results

Task
1.Plasmid extraction
2.Transform competent cells (BL21)
3.Selection of BL21(DE3) positive colony and inoculate in 10 mL liquid LB medium

Task
1.Expansion of BL21 bacterial culture
2.Induction of protein expression with IPTG
3.Synthesis of AuNPs
4.Preparation of SDS-PAGE gel
Results

Task
1.Protein extraction and purification
2.SDS-PAGE gel Electrophoresis
3.Buffer exchange of purified protein
Results

Task
1.AuNPs@PBP preparation and detection
2.Cultivate bacteria for subsequent detection (Escherichia coli, Salmonella enterica, Staphylococcus aureus)
3.Gradient dilution of detection bacterial
Results

Task
1. Colorimetric detection of bacterial by AuNPs@PBP
Results

Task
1.Summary and reporting of results of phased experiments
2.RGB Analytics and Data Analysis
Results

Task
1.Photothermal sterilisation test
2.LB solid medium preparation and flatbed coating operation
Results

Task
1.Data aggregation and analysis
2.Check the national food safety standards and determine the testing standards
3.Design icon for app design
Results

Task
1.Discuss the features that need to be implemented in a smartphone app for bacteria detection
2.Participate in the design of the APP under the guidance of an external tutor
Results