1. Personal Protective Equipment (PPE):

Always wear appropriate PPE, including lab coats, gloves, safety goggles, and face masks, as required. PPE helps protect against exposure to biological agents and chemicals.

2. Biological Agent Handling:

Follow protocols for handling biological materials, including bacteria, viruses, fungi, and cell cultures.

Use biological safety cabinets (BSCs) for procedures involving infectious agents to contain aerosols and protect the environment and personnel.

3. Spill Management:

Have spill kits readily available and know how to use them. Immediately contain and clean up spills of biological materials using appropriate disinfectants and procedures.

4. Waste Disposal:

Dispose of biological waste, including sharps, cultures, and contaminated materials, according to established guidelines and regulations.

5. Decontamination:

Decontaminate work surfaces, equipment, and spills regularly using effective disinfectants and methods appropriate for handling biological agents.

6. Emergency Preparedness:

Know the location of emergency equipment, such as eyewash stations, safety showers, fire extinguishers, and emergency exits.

Understand and practice emergency response procedures for biological hazards, including spill cleanup and evacuation protocols.

7. Training and Documentation:

Ensure all personnel receive adequate training in biological safety practices, including specific SOPs for handling different biological agents.

Maintain accurate records of training, procedures, and risk assessments.

8. Security and Access Control:

Limit access to biological laboratories to authorized personnel only.

Implement security measures to prevent unauthorized access to biological materials and sensitive information.

9. Reporting and Communication:

Report any incidents, accidents, or near misses involving biological agents promptly to laboratory supervisors or safety officers.

Foster a culture of open communication regarding safety concerns and lessons learned from incidents.

By adhering strictly to these biological laboratory safety guidelines, researchers can minimize risks associated with biological hazards and ensure a safe working environment for all laboratory personnel.

1. First, when operating E. coli, use sterilized equipment and reagents, such as media, pipettes, test tubes, etc.

2. Secondly, the laboratory must be kept clean and hygienic, and regular disinfection must be carried out to avoid contaminating the experimental materials with bacteria and dust in the air.

3. Then, to avoid cross-contamination, an E. coli operation should be carried out in accordance with the standardized operating process.

4. The operator should then pay attention to personal hygiene, wash hands frequently, and avoid bringing outside bacteria into the laboratory.

5. Finally, the E. coli strains are regularly identified to ensure the purity and activity of the strains.