Parts

PART COLLECTION

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This year, the OKstate iGEM 2024 team’s goal is to utilize synthetic biology to develop a novel treatment method targeting multi-drug resistant Staphylococcus aureus infections. We aim to accomplish this by combining two alternative antibacterial approaches. The first method is based on the concept that bacteriophages, which are viruses that targets only bacteria, can be altered to recognize specific species of bacteria, which in this case will be S. aureus. The second method is called Photodynamic Therapy (PDT) and is based on the idea that photosensitizer dyes can release antibacterial reactive oxygen species (ROS) when activated by lights at specific wavelengths.

Our basic parts collection includes the coding sequence for the E. coli DsbA signaling peptide, which is required for the translocation of any recombinant proteins across the Sec secretion machinery in E. coli. In our project, this signaling peptide is designed to be fused at the N’terminal end of our chimeric phage proteins Gp3 or Gp8. Gp3 and Gp8 are phage proteins that encode for the tail of the M13 phage. Gp3 in particular has been used for phage display studies. We have created basic parts for these two proteins. In addition, between the signaling peptide and the phage proteins, we have created three basic parts which encode for short polypeptides that are known to bind specifically to S. aureus.

BASIC PARTS USED

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Type	Part Number	Name	Description	New/Existing
Basic	BBa_J23119	Constitutive Promoter	Strong Promotor	existing
Basic	BBa_J428032	rbs	n/a	existing
Basic	BBa_K5444000	Dsba peptide	Secretion signal (Sec)	new
Basic	BBa_K5444001	SA targeting peptide 1	SSYGGSS	new
Basic	BBa_K5444002	SA targeting peptide 2	RVRSAPSSS	new
Basic	BBa_K5444003	SA targeting peptide 3	GIGKFLHSAGKFGKAFVGEIMKS	new
Basic	BBa_K5444004	Chimeric Gp3	M13 tail protein	new
Basic	BBa_K5444005	Chimeric Gp4	M13 coat protein	new
Basic	BBa_J434007	terminator	Transcriptional stop	existing

COMPOSITE PARTS USED

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Type	Part Number	Name	Description	New/Existing
Composite	BBa_K5444006	DsbA-SA peptide 1-Gp3	S. aureus targeting peptide 1 fused to Gp3	new
Composite	BBa_K5444007	DsbA-SA peptide 2-Gp3	S. aureus targeting peptide 2 fused to Gp3	new
Composite	BBa_K5444008	DsbA-SA peptide 3-Gp3	S. aureus targeting peptide 3 fused to Gp3	new
Composite	BBa_K5444009	DsbA-SA peptide 1-Gp8	S. aureus targeting peptide 1 fused to Gp8	new
Composite	BBa_K5444010	DsbA-SA peptide 2-Gp8	S. aureus targeting peptide 2 fused to Gp8	new
Composite	BBa_K5444011	DsbA-SA peptide 3-Gp8	S. aureus targeting peptide 3 fused to Gp8	new