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Overview

Our laboratory is Synthetic Biology and Biofabrication Laboratory from National Taiwan University’s department of Biochemical Engineering, and it is a Biosafety Level 1 lab operated by Professor Hsuan-Chen Wu. [1] A biochemical laboratory conducting research on microorganisms must adhere to strict safety protocols to prevent contamination, accidental exposure, and the spread of potentially harmful pathogens. Key aspects of the safety policy include proper use of personal protective equipment (PPE) such as lab coats, gloves, and safety goggles to minimize direct contact with hazardous materials. All personnel must undergo comprehensive training on handling microorganisms, including aseptic techniques, disposal of biological waste, and emergency procedures. The lab should maintain a well-ventilated environment with biosafety cabinets for working with pathogenic organisms. Clear labeling of all chemicals and biological samples is essential, as is regular decontamination of surfaces and equipment. Adherence to these guidelines ensures a safe environment for both researchers and the surrounding community.

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Project Safety

When conducting experiments involving microorganisms such as Escherichia coli (E. coli), a robust safety policy is essential to protect laboratory personnel and prevent environmental contamination. Researchers must wear appropriate personal protective equipment (PPE), including lab coats, gloves, and eye protection, to minimize direct exposure to the bacteria. Proper sterilization techniques, such as autoclaving and disinfecting surfaces, must be rigorously followed before and after handling E. coli cultures. All experiments should take place within a biosafety level 1 (BSL-1) lab, or higher if dealing with pathogenic strains, where containment procedures are in place. Waste materials, including biological samples and contaminated equipment, must be disposed of according to biohazard regulations to avoid accidental release into the environment. Additionally, personnel should be trained in emergency procedures for handling accidental spills or exposure, ensuring safe and controlled experimentation.

Figure . Potential hazards caused by genetically engineered bacteria

References

more information

[1] Associate Professor Hsuan-Chen Wu. (n.d.). Dept. Of Biochemical Science & Technology, NTU. https://www.bst.ntu.edu.tw/ntubst2019EN/News_Content_n_41872_sms_46368_s_65964.html
[2] 實驗室生物安全教育訓練資訊. (n.d.). https://www.cdc.gov.tw/Category/MPage/5PdnFt4hFcFaw2eaJus9BQ
[3] Sen-Lin Tang. (n.d.). https://biodiv.tw/pi-Sen-Lin_Tang
[4] Prof David Bourne - Research Portfolio - James Cook University. (n.d.). jcu.me. https://research.jcu.edu.au/portfolio/david.bourne/
[5] Dr Lone Høj. (n.d.). AIMS. https://www.aims.gov.au/about/our-people/dr-lone-hoj
[6] Team:Hopkins/Model - 2021.igem.org. (n.d.). https://2021.igem.org/Team:Hopkins/Model