Content
Overview
The NTU-Taiwan iGEM team worked with a total of four basic parts and four composite parts, including spider silk sequences generated by the Generative Personalized Spider Silk (GPSS) AI, which were tailored to various physical parameters. Our part numbers range from BBa_K5294001 to BBa_K5294008. The following sections focus specifically on our project and its unique contributions, rather than providing a general overview.
Basic Parts
| Part Number | Part Name | Description | Type | Length |
|---|---|---|---|---|
| BBa_K3285015 | T7 Promoter | A strong promoter that directs high levels of transcription using the T7 RNA polymerase, commonly used in bacterial expression systems. | Promoter | 19 bp |
| BBa_K1624002 | Lac operator | A sequence controlling gene expression by binding the LacI repressor; in the presence of lactose, LacI is inactivated, allowing transcription. | Operator | 25 bp |
| BBa_B0034 | RBS (Elowitz) | A well-characterized ribosome binding site that facilitates efficient translation initiation in bacteria. | RBS | 12 bp |
| BBa_K1223006 | His-Tag | A six-histidine sequence used for affinity purification of proteins, binding strongly to nickel or cobalt resins. | Tag | 18 bp |
| BBa_K3182104 | Thrombin site | A recognition sequence for the thrombin protease, used to remove tags after protein purification. | Composite | 18 bp |
| BBa_K5294002 | T9-E9-TS9-S9 Recombinant Spider silk-like protein | High tensile strength and moderate elasticity. Potential use in high-strength fibers for industrial applications (e.g., ropes, cables) | Coding | 5577 bp |
| BBa_K5294001 | T9-E9-TS9-S1 Recombinant Spider silk-like protein | Strong but with reduced elasticity. Suitable for applications requiring strength over flexibility, such as structural materials. | Coding | 2152 bp |
| BBa_K5294003 | T7-E7-TS7-S3 Recombinant Spider silk-like protein | Balanced tensile strength and flexibility. Potential use in protective clothing and sports equipment. | Coding | 2589 bp |
| BBa_K5294004 | T3-E3-TS3-S7 Recombinant Spider silk-like protein | Highly elastic with moderate strength. Ideal for applications requiring flexibility, such as medical sutures and flexible fabrics. | Coding | 912 bp |
| BBa_K2751011 | mEGFP skel | A fluorescent reporter protein often used for visualization or as a fusion tag. | Reporter | 714 bp |
| BBa_K731721 | T7 Terminator | A sequence that efficiently terminates transcription by T7 RNA polymerase, ensuring proper gene expression. | Terminator | 48 bp |
| BBa_K314110 | f1 ori | An origin of replication for single-stranded DNA production in M13 phage. | Origin | 456 bp |
| BBa_K292003 | Kanamycin resistance cassette and a double terminator | The kanamycin resistance gene, used for selecting plasmid-containing bacteria on kanamycin plates. | Composite | 816 bp |
| BBa_K2259010 | Rop protein - global copy number inhibitor (SynORI framework) | Regulates plasmid replication, controlling the copy number of the plasmid. | Coding | 192 bp |
| BBa_R0010 | promoter (lacI regulated) | Repressor protein that inhibits transcription from the Lac operator unless IPTG or lactose is present to deactivate it. | Regulatory LacI | 1083 bp |
Composite Parts
| Part Number | Description | Type | Length |
|---|---|---|---|
| BBa_K5294005 | pET28a-T7-T9E9TS9S1-mEGFP | Composite | 4152 bp |
| BBa_K5294006 | pET28a-T7-T9E9TS9S9-mEGFP | Composite | 7578 bp |
| BBa_K5294007 | pET28a-T7-T7E7TS7S3-mEGFP | Composite | 4590 bp |
| BBa_K5294008 | pET28a-T7-T3E3TS3S7-mEGFP | Composite | 2913 bp |