Parts

We successfully built 1 composite parts and 5 basic part for ligninolytic enzymes production in P. putida KT2004.

Our composite parts are based on the promoter BBa_5204002 and DNA of BBa_5204004. This composite part encodes a serious of proteins for maturing cytochrome C, which is composed of the basic parts of a strong promoter and transfaction sequence region, and related to manganese oxidation of P. putida.

Registry Code	Part Name	Short Description	Status	Length(bp)
BBa_K5204005	Engineered Ccm	Ccm operon, induce expression of cytochrome C	Successful colony PCR in P. putida KT2004.	2078

To realize our goal to construct vector allowing P. putida KT2004 to produce multiple ligninolytic enzymes, we used 5 basic parts. There were designed to induce over expression of multiple ligninolytic enzymes in pBBR1-MCS5 plasmid, incluing multicopper oxidase (MCO), manganese peroxidases (MnP) and manganese catalase (MnC). The enzymes will meanwhile stimulate the Mn²⁺ oxidation reaction to produce manganese oxide, which can be served as MnP mimics with lignocellulose degradation ability. All of them are new ones that we have submitted.

Registry Code	Part Name	Short Description	Status	Length(bp)
BBa_K5204002	Ccm_P	Strong promoter of Ccm operon	Successful colony PCR in P. putida KT2004.	50
BBa_K5204004	Ccm_T	Transcription sequence region of Ccm operon	Successful colony PCR in P. putida KT2004.	2020
BBa_K5204006	cumA	Gene that control the manganese oxidation reaction	Successful colony PCR in P. putida KT2004.	1407
BBa_K5204007	mnxG	Gene that control the manganese oxidation reaction	Successful colony PCR in P. putida KT2004.	5844
BBa_K5204008	MnP1s	Gene that control the manganese oxidation reaction	Successful colony PCR in P. putida KT2004.	1098