Contribution

In this project, we want to come up with an efficient while also innovative way to solve a large problem faced in climate crisis. In this case, mild and effective lignocellulose degradation.

Unfortunately, traditional ways to combat this challenge appeared to be low efficiency and energy-consuming. The industrial methods always require noble metal catalyst coupled with harsh reaction conditions, encompassing high temperature, high pressure, and strong acid, which is with high cost and the result is not always positive. Our method, on the other hand, has demonstrated great potential for success. With synthesized Pseudomonas putida, we are able to produce abundant ligninolytic enzymes, which, meanwhile, induce generation of manganese oxide (kind of nanozyme also with lignocellulose degradation ability) through its Mn2+ oxidation ability. Therefore, the well-designed bioreactor realizes the lignocellulose degradation in a more environmentally friendly and cost-effective way. In sum, our methodology for sure provides insight into future biological research towards climate crisis and beyond.

Cytochrome C mature protein determines the expression of multiple ligninolytic degradation enzymes, including multicopper oxidase (MCO), manganese catalase (MnC) and manganese peroxidase (MnP). We built a basic part by changing the promoter of Ccm with higher sensitivity. Then, we constructed a plasmid synthesize into Pseudomonas putida, which allows for the upregulated expression of ligninolytic degradation enzymes. This is a composite part contributed.

The bioreactor created by our team is suitable for multiple settings, home, cars, office alike. Another feature embodied in our bioreactor would be its ability in air purification. Due to its eco-friendly nature, our concept of bioreactor is able to contribute to environmental protection while also providing psychological benefits. We shed light into possible therapeutic usage of synthetically manipulated cyanobacteria and other photosynthetic organisms.