Model

Conventionally, the content of lignocellulose is reflected by the concentration of lignin, which is determined by using OD (Optical Density). This was realized by measurement of the UV-vis absorbance value at a given wavelength of 280 nm through a translucent medium. However, this requires the use of specially calibrated laboratory equipment and controlled conditions that are unlikely to be found at home. ^[1]

Thus, we use software and math models to establish a standard curve relative to the lignin content. Detailly, we prepared lignin (Dealkaline) solutions at concentrations of 0.1 mg/mL, 0.08 mg/mL, 0.07 mg/mL, 0.06 mg/mL, 0.05 mg/mL, 0.04 mg/mL, 0.03 mg/mL, 0.02 mg/mL, and 0.01 mg/mL. The OD₂₈₀ was measured and recorded for each test. The curve representing the changes of the OD value along with concentration variation was established as follows:

Based on the measured results, we established the standard curve equation:

Where y represents the absorbance of the solution at 280 nm, X represents the concentration of lignin (mg/mL).

The solution of corn straw before and after treatment were measured at 280 nm. The concentration was determined from the absorbance value, and the degradation rate in each experimental group was calculated using the following formula:

Where C₀ is the absorbance at 280 nm before treatment, C₁ is the absorbance of at 280 nm after treatment.